Product: Connexin 45 / GJA7 Antibody
Catalog: DF4080
Description: Rabbit polyclonal antibody to Connexin 45 / GJA7
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 45 KD; 45kD(Calculated).
Uniprot: P36383
RRID: AB_2836454

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(88%), Horse(88%), Rabbit(88%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
Connexin 45 / GJA7 Antibody detects endogenous levels of total Connexin 45 / GJA7.
RRID:
AB_2836454
Cite Format: Affinity Biosciences Cat# DF4080, RRID:AB_2836454.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Connexin 45; Connexin-45; Connexin45; CX 31.9; CX 45; CX31.9; Cx45; CXG1_HUMAN; DKFZp686P0738; Gap junction alpha 7 protein; Gap junction alpha-7 protein; Gap junction gamma 1 protein; Gap junction gamma-1 protein; Gap junction protein alpha 7 45kDa; Gap junction protein alpha 7; Gap junction protein, gamma 1, 45kDa; GJA 7; GJA7; GJC1; GJD3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSWSFLTRLLEEIHNHSTFVGKIWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTEQPGCENVCYDAFAPLSHVRFWVFQIILVATPSVMYLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETEEDNEEDPMMYPEMELESDKENKEQSQPKPKHDGRRRIREDGLMKIYVLQLLARTVFEVGFLIGQYFLYGFQVHPFYVCSRLPCPHKIDCFISRPTEKTIFLLIMYGVTGLCLLLNIWEMLHLGFGTIRDSLNSKRRELEDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANTAQEQQYGSHEENLPADLEALQREIRMAQERLDLAVQAYSHQNNPHGPREKKAKVGSKAGSNKSTASSKSGDGKTSVWI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Horse
88
Bovine
88
Rabbit
88
Chicken
83
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36383 As Substrate

Site PTM Type Enzyme
K115 Methylation
Y141 Phosphorylation
S148 Phosphorylation
K150 Ubiquitination
K295 Ubiquitination
Y301 Phosphorylation
K308 Ubiquitination
K312 Ubiquitination
K315 Ubiquitination
Y324 Phosphorylation
Y356 Phosphorylation
S357 Phosphorylation
K371 Acetylation
K380 Ubiquitination
K391 Ubiquitination

Research Backgrounds

Function:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

A connexon is composed of a hexamer of connexins. Interacts with CNST (By similarity).

Family&Domains:

Belongs to the connexin family. Gamma-type subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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