Product: GJA4 Antibody
Catalog: DF4079
Description: Rabbit polyclonal antibody to GJA4
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 37 KD; 37kD(Calculated).
Uniprot: P35212
RRID: AB_2836453

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
GJA4 Antibody detects endogenous levels of total GJA4.
RRID:
AB_2836453
Cite Format: Affinity Biosciences Cat# DF4079, RRID:AB_2836453.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Connexin 37; Connexin-37; Cx37; CXA4_HUMAN; Gap junction alpha 4 protein; Gap junction alpha-4 protein; gap junction protein, alpha 4 37kDa (connexin 37); gap junction protein, alpha 4, 37kD (connexin 37); Gap junction protein, alpha 4, 37kDa; Gja4; OTTHUMP00000004181; OTTHUMP00000004182;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P35212 CXA4_HUMAN:

Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.

Sequence:
MGDWGFLEKLLDQVQEHSTVVGKIWLTVLFIFRILILGLAGESVWGDEQSDFECNTAQPGCTNVCYDQAFPISHIRYWVLQFLFVSTPTLVYLGHVIYLSRREERLRQKEGELRALPAKDPQVERALAAVERQMAKISVAEDGRLRIRGALMGTYVASVLCKSVLEAGFLYGQWRLYGWTMEPVFVCQRAPCPYLVDCFVSRPTEKTIFIIFMLVVGLISLVLNLLELVHLLCRCLSRGMRARQGQDAPPTQGTSSDPYTDQVFFYLPVGQGPSSPPCPTYNGLSSSEQNWANLTTEERLASSRPPLFLDPPPQNGQKPPSRPSSSASKKQYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
92
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35212 As Substrate

Site PTM Type Enzyme
K136 Acetylation
P319 Phosphorylation

Research Backgrounds

Function:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.

Subunit Structure:

A connexon is composed of a hexamer of connexins.

Family&Domains:

Belongs to the connexin family. Alpha-type (group II) subfamily.

References

1). Effect of Cyclic Adenosine Monophosphate on Connexin 37 Expression in Sheep Cumulus-Oocyte Complexes. Journal of developmental biology, 2024 (PubMed: 38651455) [IF=2.7]

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