GGH Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from RAW264.

Product:	GGH Antibody
Catalog:	DF4073
Description:	Rabbit polyclonal antibody to GGH
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.:	36 KD; 36kD(Calculated).
Uniprot:	Q92820
RRID:	AB_2836447

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Zebrafish(89%), Bovine(80%), Horse(100%), Sheep(80%), Dog(80%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

GGH Antibody detects endogenous levels of total GGH.

RRID:

AB_2836447
Cite Format: Affinity Biosciences Cat# DF4073, RRID:AB_2836447.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Conjugase; folylpolygammaglutamyl hydrolase; gamma glutamyl hydrolase; Gamma-Glu-X carboxypeptidase; Gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase); Gamma-glutamyl hydrolase; GGH; GGH_HUMAN; gH;

Immunogens

Immunogen:

Uniprot:

Q92820(GGH_HUMAN) >>Visit HPA database.

Gene(ID):

GGH(8836)

Sequence:

Q92820 GGH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASPGCLLCVLGLLLCGAASLELSRPHGDTAKKPIIGILMQKCRNKVMKNYGRYYIAASYVKYLESAGARVVPVRLDLTEKDYEILFKSINGILFPGGSVDLRRSDYAKVAKIFYNLSIQSFDDGDYFPVWGTCLGFEELSLLISGECLLTATDTVDVAMPLNFTGGQLHSRMFQNFPTELLLSLAVEPLTANFHKWSLSVKNFTMNEKLKKFFNVLTTNTDGKIEFISTMEGYKYPVYGVQWHPEKAPYEWKNLDGISHAPNAVKTAFYLAEFFVNEARKNNHHFKSESEEEKALIYQFSPIYTGNISSFQQCYIFD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Zebrafish

Bovine

Sheep

Dog

Xenopus

Chicken

Pig

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92820 As Substrate

Q92820

Site	PTM Type	Source
K81	Ubiquitination	Uniprot
K109	Ubiquitination	Uniprot
N163	N-Glycosylation	Uniprot
N203	N-Glycosylation	Uniprot
K212	Ubiquitination	Uniprot
K235	Ubiquitination	Uniprot
K253	Ubiquitination	Uniprot
K287	Ubiquitination	Uniprot
N307	N-Glycosylation	Uniprot

Research Backgrounds

Q92820_GGH_HUMAN

Function:

Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates.

Subcellular Location:

Secreted>Extracellular space. Lysosome. Melanosome.
Note: While its intracellular location is primarily the lysosome, most of the enzyme activity is secreted. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the peptidase C26 family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

Restrictive clause

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For Research Use Only.
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GGH Antibody - #DF4073