FUT3 Antibody - #DF4068

Figure 5 Fucosylation of EV-Hp is upregulated by CCA. (A) IHC staining of β-Hp in CCA tissue. Scale bar = 50 μm. IHC scores are shown. (B) IHC staining of TSG101 and CD63 in CCA tissue. Scale bar = 50 μm. IHC scores are shown. (C) IHC staining of FUT3 and FUT4 in CCA tissue. Scale bar = 50 μm. IHC score is shown. Quantitative data are shown as mean ± S.E.M. Statistical analysis was performed using the Mann-Whitney u-test. ****p < 0.0001, **p < 0.01, *p < 0.05, ns p > 0.05. ns, not significant.

Figure 5 FUT3 knockdown in RL95-2 cells decreases the IL-1β induced spheroids adhesion and surface expression of sLeX. (A and B) RT-qPCR and western blot analysis indicated that FUT3 siRNA transfection significantly decreased the mRNA and protein expression of the target gene at both the basal and IL-1β (20 ng/ml) stimulated condition, n = 3 per group. (C) FUT3 knockdown decreased the basal and IL-1β induced spheroids adhesion to RL95-2 cells monolayer, n = 9 per group. The percentage of adhesion denotes the proportion of attached HTR-8/SVneo spheroids in the total number of initially transferred spheroids into each chamber. Raw data from the spheroids adhesion experiment can be found in Supplemental Table 2. (D and E) Flow cytometry suggested a substantial reduction of surface sLeX but not LeY expression by FUT3 siRNA transfection, n = 3 per group. Numbers by the representative histograms of isotype control IgM and specific sLeX/LeY staining indicate the positive cells (%). Data represent mean ± SEM. ANOVA followed by Tukey’s post hoc tests were performed for statistical analysis of three independent experiments. si-NC, non-target siRNA.