ALDOB Antibody - #DF4064

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Product: ALDOB Antibody
Catalog: DF4064
Description: Rabbit polyclonal antibody to ALDOB
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit, Dog
Mol.Wt.: 39 KD; 39kD(Calculated).
Uniprot: P05062
RRID: AB_2836438

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Horse(83%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
ALDOB Antibody detects endogenous levels of total ALDOB.
RRID:
AB_2836438
Cite Format: Affinity Biosciences Cat# DF4064, RRID:AB_2836438.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ALDB; ALDO B; ALDO2; ALDOB; ALDOB_HUMAN; Aldolase 2; Aldolase B; Aldolase B fructose bisphosphate; Aldolase2; AldolaseB; EC 4.1.2.13; Fructose bisphosphate aldolase B; Fructose-bisphosphate aldolase B; Liver type aldolase; Liver-type aldolase; MS1077;

Immunogens

Immunogen:
Uniprot:

P05062(ALDOB_HUMAN) >>Visit HPA database.

Gene(ID):
ALDOB(229)
Sequence:
MAHRFPALTQEQKKELSEIAQSIVANGKGILAADESVGTMGNRLQRIKVENTEENRRQFREILFSVDSSINQSIGGVILFHETLYQKDSQGKLFRNILKEKGIVVGIKLDQGGAPLAGTNKETTIQGLDGLSERCAQYKKDGVDFGKWRAVLRIADQCPSSLAIQENANALARYASICQQNGLVPIVEPEVIPDGDHDLEHCQYVTEKVLAAVYKALNDHHVYLEGTLLKPNMVTAGHACTKKYTPEQVAMATVTALHRTVPAAVPGICFLSGGMSEEDATLNLNAINLCPLPKPWKLSFSYGRALQASALAAWGGKAANKEATQEAFMKRAMANCQAAKGQYVHTGSSGAASTQSLFTACYTY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
92
Horse
83
Bovine
83
Sheep
75
Xenopus
67
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05062 As Substrate

Site PTM Type Enzyme
K13 Acetylation
S36 Phosphorylation
T39 Phosphorylation
S89 Phosphorylation
T119 Phosphorylation
T123 Phosphorylation
T124 Phosphorylation
S132 Phosphorylation
K139 Acetylation
K147 Acetylation
S161 Phosphorylation
Y204 Phosphorylation
T206 Phosphorylation
K208 Acetylation
K208 Ubiquitination
Y214 Phosphorylation
K215 Methylation
Y223 Phosphorylation
T241 Phosphorylation
K242 Acetylation
K243 Acetylation
C269 S-Nitrosylation
S272 Phosphorylation
S276 Phosphorylation
Y302 Phosphorylation
S309 Phosphorylation
K330 Acetylation
Y362 Phosphorylation
T363 Phosphorylation

Research Backgrounds

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer. Interacts with BBS1, BBS2, BBS4 and BBS7.

Family&Domains:

Belongs to the class I fructose-bisphosphate aldolase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

References

1). SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. FREE RADICAL BIOLOGY AND MEDICINE, 2019 (PubMed: 30703481) [IF=7.4]

Application: WB    Species: human    Sample: kidney

Fig. S2.| Verification of the differential succinylation of proteins found by MS/MS assay. These proteins were immunoprecipitated by corresponding antibodies and detected with pan anti-succinyllysine antibody. Adj, adjacent normal tissues. T, ccRCC tissues.

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