EIF5B Antibody - #DF4055

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Product: EIF5B Antibody
Catalog: DF4055
Description: Rabbit polyclonal antibody to EIF5B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 139 KD; 139kD(Calculated).
Uniprot: O60841
RRID: AB_2836429

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
EIF5B Antibody detects endogenous levels of total EIF5B.
RRID:
AB_2836429
Cite Format: Affinity Biosciences Cat# DF4055, RRID:AB_2836429.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp434I036; eIF 5B; eIF-5B; Eif5b; Eukaryotic translation initiation factor 5B; FLJ10524; IF2; IF2P_HUMAN; KIAA0741; Translation initiation factor IF 2; Translation initiation factor IF-2; Translation initiation factor IF2;

Immunogens

Immunogen:
Uniprot:

O60841(IF2P_HUMAN) >>Visit HPA database.

Gene(ID):
EIF5B(9669)
Sequence:
MGKKQKNKSEDSTKDDIDLDALAAEIEGAGAAKEQEPQKSKGKKKKEKKKQDFDEDDILKELEELSLEAQGIKADRETVAVKPTENNEEEFTSKDKKKKGQKGKKQSFDDNDSEELEDKDSKSKKTAKPKVEMYSGSDDDDDFNKLPKKAKGKAQKSNKKWDGSEEDEDNSKKIKERSRINSSGESGDESDEFLQSRKGQKKNQKNKPGPNIESGNEDDDASFKIKTVAQKKAEKKERERKKRDEEKAKLRKLKEKEELETGKKDQSKQKESQRKFEEETVKSKVTVDTGVIPASEEKAETPTAAEDDNEGDKKKKDKKKKKGEKEEKEKEKKKGPSKATVKAMQEALAKLKEEEERQKREEEERIKRLEELEAKRKEEERLEQEKRERKKQKEKERKERLKKEGKLLTKSQREARARAEATLKLLQAQGVEVPSKDSLPKKRPIYEDKKRKKIPQQLESKEVSESMELCAAVEVMEQGVPEKEETPPPVEPEEEEDTEDAGLDDWEAMASDEETEKVEGNKVHIEVKENPEEEEEEEEEEEEDEESEEEEEEEGESEGSEGDEEDEKVSDEKDSGKTLDKKPSKEMSSDSEYDSDDDRTKEERAYDKAKRRIEKRRLEHSKNVNTEKLRAPIICVLGHVDTGKTKILDKLRHTHVQDGEAGGITQQIGATNVPLEAINEQTKMIKNFDRENVRIPGMLIIDTPGHESFSNLRNRGSSLCDIAILVVDIMHGLEPQTIESINLLKSKKCPFIVALNKIDRLYDWKKSPDSDVAATLKKQKKNTKDEFEERAKAIIVEFAQQGLNAALFYENKDPRTFVSLVPTSAHTGDGMGSLIYLLVELTQTMLSKRLAHCEELRAQVMEVKALPGMGTTIDVILINGRLKEGDTIIVPGVEGPIVTQIRGLLLPPPMKELRVKNQYEKHKEVEAAQGVKILGKDLEKTLAGLPLLVAYKEDEIPVLKDELIHELKQTLNAIKLEEKGVYVQASTLGSLEALLEFLKTSEVPYAGINIGPVHKKDVMKASVMLEHDPQYAVILAFDVRIERDAQEMADSLGVRIFSAEIIYHLFDAFTKYRQDYKKQKQEEFKHIAVFPCKIKILPQYIFNSRDPIVMGVTVEAGQVKQGTPMCVPSKNFVDIGIVTSIEINHKQVDVAKKGQEVCVKIEPIPGESPKMFGRHFEATDILVSKISRQSIDALKDWFRDEMQKSDWQLIVELKKVFEII

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60841 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
S12 Phosphorylation
T13 Phosphorylation
K33 Ubiquitination
K50 Ubiquitination
S66 Phosphorylation
K73 Ubiquitination
T78 Phosphorylation
S93 Phosphorylation
S107 Phosphorylation
S113 Phosphorylation
S121 Phosphorylation
Y134 Phosphorylation
S135 Phosphorylation
S137 Phosphorylation
S164 Phosphorylation
S171 Phosphorylation
S178 Phosphorylation
S182 Phosphorylation
S183 Phosphorylation
S186 Phosphorylation
S190 Phosphorylation
S196 Phosphorylation
S214 Phosphorylation
S222 Phosphorylation
K284 Ubiquitination
S295 Phosphorylation
T301 Phosphorylation
T303 Phosphorylation
K375 Ubiquitination
S411 Phosphorylation
K424 Ubiquitination
S435 Phosphorylation
S438 Phosphorylation
Y446 Phosphorylation
S460 Phosphorylation
T486 Phosphorylation
T498 Phosphorylation
S511 Phosphorylation
T515 Phosphorylation
S547 Phosphorylation
S557 Phosphorylation
S560 Phosphorylation
S584 Phosphorylation
S588 Phosphorylation
S589 Phosphorylation
S591 Phosphorylation
Y593 Phosphorylation
S595 Phosphorylation
K745 Ubiquitination
Y762 Phosphorylation
K766 Ubiquitination
S767 Phosphorylation
S770 Phosphorylation
T775 Phosphorylation
K784 Acetylation
K883 Ubiquitination
K923 Acetylation
K923 Ubiquitination
K932 Ubiquitination
K936 Ubiquitination
K968 Acetylation
K968 Ubiquitination
T1000 Phosphorylation
S1001 Phosphorylation
C1092 S-Nitrosylation
K1093 Ubiquitination
K1095 Ubiquitination
Y1100 Phosphorylation
S1104 Phosphorylation
K1153 Ubiquitination
K1160 Acetylation
S1168 Phosphorylation
K1170 Acetylation
K1170 Ubiquitination
S1184 Phosphorylation
K1185 Ubiquitination
K1214 Acetylation

Research Backgrounds

Function:

Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.

PTMs:

(Microbial infection) Cleaved and inactivated by the protease 3C of poliovirus, Coxsackievirus B3 and Human rhinovirus 14, allowing the virus to shutoff the host cell translation.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ANXA5 in a calcium and phospholipid-dependent manner.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

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