Product: EPN3 Antibody
Catalog: DF4046
Description: Rabbit polyclonal antibody to EPN3
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 68 KD; 68kD(Calculated).
Uniprot: Q9H201
RRID: AB_2836420

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
EPN3 Antibody detects endogenous levels of total EPN3.
RRID:
AB_2836420
Cite Format: Affinity Biosciences Cat# DF4046, RRID:AB_2836420.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EPN 3; EPS 15 interacting protein 3; Epsin 3; Epsin3; FLJ20778; MGC129899;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9H201 EPN3_HUMAN:

Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin. Detected in chronic wounds, basal cell carcinoma and ulcerative colitis.

Sequence:
MTTSALRRQVKNIVHNYSEAEIKVREATSNDPWGPPSSLMSEIADLTFNTVAFTEVMGMLWRRLNDSGKNWRHVYKALTLLDYLLKTGSERVAHQCRENLYTIQTLKDFQYIDRDGKDQGVNVREKVKQVMALLKDEERLRQERTHALKTKERMALEGIGIGSGQLGFSRRYGEDYSRSRGSPSSYNSSSSSPRYTSDLEQARPQTSGEEELQLQLALAMSREEAEKPVPPASHRDEDLQLQLALRLSRQEHEKEVRSWQGDGSPMANGAGAVVHHQRDREPEREERKEEEKLKTSQSSILDLADIFVPALAPPSTHCSADPWDIPGFRPNTEASGSSWGPSADPWSPIPSGTVLSRSQPWDLTPMLSSSEPWGRTPVLPAGPPTTDPWALNSPHHKLPSTGADPWGASLETSDTPGGASTFDPFAKPPESTETKEGLEQALPSGKPSSPVELDLFGDPSPSSKQNGTKEPDALDLGILGEALTQPSKEARACRTPESFLGPSASSLVNLDSLVKAPQVAKTRNPFLTGLSAPSPTNPFGAGEPGRPTLNQMRTGSPALGLAGGPVGAPLGSMTYSASLPLPLSSVPAGLTLPASVSVFPQAGAFAPQPLLPTPSSAGPRPPPPQTGTNPFL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
91
Xenopus
50
Chicken
42
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H201 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
T3 Phosphorylation
S4 Phosphorylation
Y17 Phosphorylation
S18 Phosphorylation
K23 Ubiquitination
Y83 Phosphorylation
Y101 Phosphorylation
T102 Phosphorylation
Y111 Phosphorylation
K117 Ubiquitination
K149 Ubiquitination
S163 Phosphorylation
S169 Phosphorylation
Y172 Phosphorylation
Y176 Phosphorylation
S177 Phosphorylation
S182 Phosphorylation
Y186 Phosphorylation
Y195 Phosphorylation
T206 Phosphorylation
S207 Phosphorylation
S258 Phosphorylation
S264 Phosphorylation
S358 Phosphorylation
S368 Phosphorylation
S393 Phosphorylation
S444 Phosphorylation
S460 Phosphorylation
T495 Phosphorylation
S498 Phosphorylation
S534 Phosphorylation

Research Backgrounds

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Cytoplasmic vesicle>Clathrin-coated vesicle. Nucleus.
Note: Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the cell periphery. May shuttle to the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin. Detected in chronic wounds, basal cell carcinoma and ulcerative colitis.

Family&Domains:

Belongs to the epsin family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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