NDUFB9 Antibody | Affinity Biosciences

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Western blot analysis of extracts from COLO cells using NDUFB9 antibody.

Product:	NDUFB9 Antibody
Catalog:	DF4208
Description:	Rabbit polyclonal antibody to NDUFB9
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	22 KD; 22kD(Calculated).
Uniprot:	Q9Y6M9
RRID:	AB_2836416

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(91%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

NDUFB9 Antibody detects endogenous levels of total NDUFB9.

RRID:

AB_2836416
Cite Format: Affinity Biosciences Cat# DF4208, RRID:AB_2836416.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

B22; CI B22; CI-B22; complex I B22 subunit; Complex I-B22; DKFZp566O173; FLJ22885; I B22; LYR motif containing protein 3; LYR motif-containing protein 3; LYRM3; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; NADH ubiquinone oxidoreductase B22 subunit; NADH-ubiquinone oxidoreductase B22 subunit; NDUB9_HUMAN; Ndufb9; UQOR22;

Immunogens

Immunogen:

Uniprot:

Q9Y6M9(NDUB9_HUMAN) >>Visit HPA database.

Gene(ID):

NDUFB9(4715)

Sequence:

Q9Y6M9 NDUB9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAFLASGPYLTHQQKVLRLYKRALRHLESWCVQRDKYRYFACLMRARFEEHKNEKDMAKATQLLKEAEEEFWYRQHPQPYIFPDSPGGTSYERYDCYKVPEWCLDDWHPSEKAMYPDYFAKREQWKKLRRESWEREVKQLQEETPPGGPLTEALPPARKEGDLPPLWWYIVTRPRERPM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Pig

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6M9 As Substrate

Q9Y6M9

Site	PTM Type	Source
A2	Acetylation	Uniprot
S6	Phosphorylation	Uniprot
Y9	Phosphorylation	Uniprot
K15	Ubiquitination	Uniprot
K55	Acetylation	Uniprot
K59	Ubiquitination	Uniprot
K65	Ubiquitination	Uniprot
Y73	Phosphorylation	Uniprot
Y80	Phosphorylation	Uniprot
S85	Phosphorylation	Uniprot
T89	Phosphorylation	Uniprot
Y91	Phosphorylation	Uniprot
K98	Acetylation	Uniprot
K98	Ubiquitination	Uniprot
K112	Ubiquitination	Uniprot
Y115	Phosphorylation	Uniprot
Y118	Phosphorylation	Uniprot
K121	Acetylation	Uniprot
K121	Ubiquitination	Uniprot
S132	Phosphorylation	Uniprot
K138	Ubiquitination	Uniprot
Y169	Phosphorylation	Uniprot

Research Backgrounds

Q9Y6M9_NDUB9_HUMAN

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Subunit Structure:

Mammalian complex I is composed of 45 different subunits.

Family&Domains:

Belongs to the complex I LYR family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

Restrictive clause

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For Research Use Only.
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NDUFB9 Antibody - #DF4208