NDUFB10 Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from COLO cells using NDUFB10 antibody.

Product:	NDUFB10 Antibody
Catalog:	DF4207
Description:	Rabbit polyclonal antibody to NDUFB10
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.:	24 KD; 21kD(Calculated).
Uniprot:	O96000
RRID:	AB_2836415

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Zebrafish(91%), Bovine(91%), Horse(91%), Sheep(100%), Dog(91%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

NDUFB10 Antibody detects endogenous levels of total NDUFB10.

RRID:

AB_2836415
Cite Format: Affinity Biosciences Cat# DF4207, RRID:AB_2836415.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

0610011B04Rik; 22kDa; CI PDSW; CI-PDSW; Complex I PDSW; Complex I PDSW subunit; Complex I-PDSW; NADH dehydrogenase (ubiquinone) 1 beta subcomplex 10 22kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; NADH ubiquinone oxidoreductase PDSW subunit; NADH-ubiquinone oxidoreductase PDSW subunit; NDUBA_HUMAN; NDUFB 10; Ndufb10; PDSW;

Immunogens

Immunogen:

Uniprot:

O96000(NDUBA_HUMAN) >>Visit HPA database.

Gene(ID):

NDUFB10(4716)

Sequence:

O96000 NDUBA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPDSWDKDVYPEPPRRTPVQPNPIVYMMKAFDLIVDRPVTLVREFIERQHAKNRYYYYHRQYRRVPDITECKEEDIMCMYEAEMQWKRDYKVDQEIINIMQDRLKACQQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQERKAAKEAAAATS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Sheep

100

Xenopus

100

Pig

Horse

Bovine

Dog

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O96000 As Substrate

O96000

Site	PTM Type	Source
S4	Phosphorylation	Uniprot
K7	Ubiquitination	Uniprot
T17	Phosphorylation	Uniprot
Y26	Phosphorylation	Uniprot
Y55	Phosphorylation	Uniprot
Y56	Phosphorylation	Uniprot
Y57	Phosphorylation	Uniprot
K91	Ubiquitination	Uniprot
K105	Ubiquitination	Uniprot
K121	Ubiquitination	Uniprot
K131	Ubiquitination	Uniprot
R136	Methylation	Uniprot
Y137	Phosphorylation	Uniprot
Y143	Phosphorylation	Uniprot
S145	Phosphorylation	Uniprot
K148	Ubiquitination	Uniprot

Research Backgrounds

O96000_NDUBA_HUMAN

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein>Matrix side.

Subunit Structure:

Complex I is composed of 45 different subunits.

Family&Domains:

Belongs to the complex I NDUFB10 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

NDUFB10 Antibody - #DF4207