NDUFA8 Antibody - #DF4205

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Product: NDUFA8 Antibody
Catalog: DF4205
Description: Rabbit polyclonal antibody to NDUFA8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 22 KD; 20kD(Calculated).
Uniprot: P51970
RRID: AB_2836413

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
NDUFA8 Antibody detects endogenous levels of total NDUFA8.
RRID:
AB_2836413
Cite Format: Affinity Biosciences Cat# DF4205, RRID:AB_2836413.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CI 19KD; CI PGIV; CI-19kD; CI-PGIV; Complex I 19k; Complex I 19kD; Complex I PGIV; Complex I PGIV subunit; Complex I-19kD; Complex I-PGIV; MGC793; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 (19kD PGIV); NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8 19kDa; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 8; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; NADH ubiquinone oxidoreductase 19 kDa subunit; NADH ubiquinone oxidoreductase PGIV subunit; NADH-ubiquinone oxidoreductase 19 kDa subunit; NDUA8_HUMAN; NDUFA 8; NDUFA8; OTTHUMP00000022034; PGIV;

Immunogens

Immunogen:
Uniprot:

P51970(NDUA8_HUMAN) >>Visit HPA database.

Gene(ID):
NDUFA8(4702)
Sequence:
MPGIVELPTLEELKVDEVKISSAVLKAAAHHYGAQCDKPNKEFMLCRWEEKDPRRCLEEGKLVNKCALDFFRQIKRHCAEPFTEYWTCIDYTGQQLFRHCRKQQAKFDECVLDKLGWVRPDLGELSKVTKVKTDRPLPENPYHSRPRPDPSPEIEGDLQPATHGSRFYFWTK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Chicken
91
Zebrafish
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51970 As Substrate

Site PTM Type Enzyme
K14 Ubiquitination
K19 Ubiquitination
K26 Ubiquitination
K38 Acetylation
K38 Ubiquitination
K41 Ubiquitination
K51 Ubiquitination
K61 Ubiquitination
K65 Ubiquitination
K102 Ubiquitination
K106 Ubiquitination
K114 Ubiquitination
K127 Ubiquitination
K132 Ubiquitination
Y142 Phosphorylation
S151 Phosphorylation
Y168 Phosphorylation

Research Backgrounds

Function:

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

PTMs:

May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.

Subcellular Location:

Mitochondrion inner membrane>Peripheral membrane protein. Mitochondrion intermembrane space. Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Complex I is composed of 45 different subunits.

Family&Domains:

Contains four C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.

Belongs to the complex I NDUFA8 subunit family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling.   (View pathway)

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For Research Use Only.
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