MYO9B Antibody - #DF4199

(2)  
Product: MYO9B Antibody
Catalog: DF4199
Description: Rabbit polyclonal antibody to MYO9B
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 250 KD; 243kD(Calculated).
Uniprot: Q13459
RRID: AB_2836408

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
MYO9B Antibody detects endogenous levels of total MYO9B.
RRID:
AB_2836408
Cite Format: Affinity Biosciences Cat# DF4199, RRID:AB_2836408.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CELIAC 4; Celiac disease 4; CELIAC4; MYO 9B; Myosin 9B; Myosin IX; Myosin IXB; Myosin9B; MyosinIX; MyosinIXB; MYR 5; MYR5; Unconventional myosin 9b;

Immunogens

Immunogen:
Uniprot:

Q13459(MYO9B_HUMAN) >>Visit HPA database.

Gene(ID):
MYO9B(4650)
Expression:
Q13459 MYO9B_HUMAN:

Detected in peripheral blood leukocytes (at protein level) (PubMed:9490638). Expressed predominantly in peripheral blood leukocytes and at lower levels, in thymus, spleen, testis, prostate, ovary, brain, small intestine and lung.

Sequence:
MSVKEAGSSGRREQAAYHLHIYPQLSTTESQASCRVTATKDSTTSDVIKDAIASLRLDGTKCYVLVEVKESGGEEWVLDANDSPVHRVLLWPRRAQDEHPQEDGYYFLLQERNADGTIKYVHMQLVAQATATRRLVERGLLPRQQADFDDLCNLPELTEGNLLKNLKHRFLQQKIYTYAGSILVAINPFKFLPIYNPKYVKMYENQQLGKLEPHVFALADVAYYTMLRKRVNQCIVISGESGSGKTQSTNFLIHCLTALSQKGYASGVERTILGAGPVLEAFGNAKTAHNNNSSRFGKFIQVSYLESGIVRGAVVEKYLLEKSRLVSQEKDERNYHVFYYLLLGVSEEERQEFQLKQPEDYFYLNQHNLKIEDGEDLKHDFERLKQAMEMVGFLPATKKQIFAVLSAILYLGNVTYKKRATGREEGLEVGPPEVLDTLSQLLKVKREILVEVLTKRKTVTVNDKLILPYSLSEAITARDSMAKSLYSALFDWIVLRINHALLNKKDVEEAVSCLSIGVLDIFGFEDFERNSFEQFCINYANEQLQYYFNQHIFKLEQEEYQGEGITWHNIGYTDNVGCIHLISKKPTGLFYLLDEESNFPHATSQTLLAKFKQQHEDNKYFLGTPVMEPAFIIQHFAGKVKYQIKDFREKNMDYMRPDIVALLRGSDSSYVRELIGMDPVAVFRWAVLRAAIRAMAVLREAGRLRAERAEKAAGMSSPGAQSHPEELPRGASTPSEKLYRDLHNQMIKSIKGLPWQGEDPRSLLQSLSRLQKPRAFILKSKGIKQKQIIPKNLLDSKSLKLIISMTLHDRTTKSLLHLHKKKKPPSISAQFQTSLNKLLEALGKAEPFFIRCIRSNAEKKELCFDDELVLQQLRYTGMLETVRIRRSGYSAKYTFQDFTEQFQVLLPKDAQPCREVISTLLEKMKIDKRNYQIGKTKVFLKETERQALQETLHREVVRKILLLQSWFRMVLERRHFLQMKRAAVTIQACWRSYRVRRALERTQAAVYLQASWRGYWQRKLYRHQKQSIIRLQSLCRGHLQRKSFSQMISEKQKAEEKEREALEAARAGAEEGGQGQAAGGQQVAEQGPEPAEDGGHLASEPEVQPSDRSPLEHSSPEKEAPSPEKTLPPQKTVAAESHEKVPSSREKRESRRQRGLEHVKFQNKHIQSCKEESALREPSRRVTQEQGVSLLEDKKESREDETLLVVETEAENTSQKQPTEQPQAMAVGKVSEETEKTLPSGSPRPGQLERPTSLALDSRVSPPAPGSAPETPEDKSKPCGSPRVQEKPDSPGGSTQIQRYLDAERLASAVELWRGKKLVAAASPSAMLSQSLDLSDRHRATGAALTPTEERRTSFSTSDVSKLLPSLAKAQPAAETTDGERSAKKPAVQKKKPGDASSLPDAGLSPGSQVDSKSTFKRLFLHKTKDKKYSLEGAEELENAVSGHVVLEATTMKKGLEAPSGQQHRHAAGEKRTKEPGGKGKKNRNVKIGKITVSEKWRESVFRQITNANELKYLDEFLLNKINDLRSQKTPIESLFIEATEKFRSNIKTMYSVPNGKIHVGYKDLMENYQIVVSNLATERGQKDTNLVLNLFQSLLDEFTRGYTKNDFEPVKQSKAQKKKRKQERAVQEHNGHVFASYQVSIPQSCEQCLSYIWLMDKALLCSVCKMTCHKKCVHKIQSHCSYTYGRKGEPGVEPGHFGVCVDSLTSDKASVPIVLEKLLEHVEMHGLYTEGLYRKSGAANRTRELRQALQTDPAAVKLENFPIHAITGVLKQWLRELPEPLMTFAQYGDFLRAVELPEKQEQLAAIYAVLEHLPEANHNSLERLIFHLVKVALLEDVNRMSPGALAIIFAPCLLRCPDNSDPLTSMKDVLKITTCVEMLIKEQMRKYKVKMEEISQLEAAESIAFRRLSLLRQNAPWPLKLGFSSPYEGVLNKSPKTRDIQEEELEVLLEEEAAGGDEDREKEILIERIQSIKEEKEDITYRLPELDPRGSDEENLDSETSASTESLLEERAGRGASEGPPAPALPCPGAPTPSPLPTVAAPPRRRPSSFVTVRVKTPRRTPIMPTANIKLPPGLPSHLPRWAPGAREAAAPVRRREPPARRPDQIHSVYITPGADLPVQGALEPLEEDGQPPGAKRRYSDPPTYCLPPASGQTNG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
73
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13459 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K4 Ubiquitination
Y17 Phosphorylation
Y22 Phosphorylation
K40 Ubiquitination
K49 Ubiquitination
S83 Phosphorylation
K198 Ubiquitination
K201 Ubiquitination
K262 Ubiquitination
S307 Phosphorylation
K322 Ubiquitination
Y339 Phosphorylation
K378 Ubiquitination
S716 Phosphorylation
S717 Phosphorylation
S722 Phosphorylation
K737 Ubiquitination
Y739 Phosphorylation
K748 Ubiquitination
S766 Phosphorylation
K797 Ubiquitination
K844 Ubiquitination
Y875 Phosphorylation
T876 Phosphorylation
T881 Phosphorylation
Y893 Phosphorylation
T919 Phosphorylation
K923 Ubiquitination
K935 Ubiquitination
K941 Ubiquitination
T951 Phosphorylation
S965 Phosphorylation
S1043 Phosphorylation
S1045 Phosphorylation
S1109 Phosphorylation
S1114 Phosphorylation
S1115 Phosphorylation
S1122 Phosphorylation
T1126 Phosphorylation
S1143 Phosphorylation
S1150 Phosphorylation
T1183 Phosphorylation
S1240 Phosphorylation
S1242 Phosphorylation
T1252 Phosphorylation
S1253 Phosphorylation
S1258 Phosphorylation
S1261 Phosphorylation
S1267 Phosphorylation
T1271 Phosphorylation
S1276 Phosphorylation
S1281 Phosphorylation
S1290 Phosphorylation
S1294 Phosphorylation
T1295 Phosphorylation
S1308 Phosphorylation
S1323 Phosphorylation
S1325 Phosphorylation
S1329 Phosphorylation
S1331 Phosphorylation
T1346 Phosphorylation P28482 (MAPK1)
T1348 Phosphorylation
T1353 Phosphorylation
S1354 Phosphorylation
S1356 Phosphorylation
S1358 Phosphorylation
S1361 Phosphorylation
S1366 Phosphorylation
S1382 Phosphorylation
S1397 Phosphorylation
S1398 Phosphorylation
S1405 Phosphorylation
S1408 Phosphorylation
S1430 Phosphorylation
K1454 Ubiquitination
K1474 Acetylation
K1479 Acetylation
K1481 Acetylation
K1490 Ubiquitination
K1496 Ubiquitination
Y1569 Phosphorylation
S1574 Phosphorylation
T1578 Phosphorylation
Y1683 Phosphorylation
T1684 Phosphorylation
Y1685 Phosphorylation
T1752 Phosphorylation
S1925 Phosphorylation
S1926 Phosphorylation
Y1928 Phosphorylation
S1935 Phosphorylation
S1972 Phosphorylation
S1992 Phosphorylation
S1999 Phosphorylation
S2004 Phosphorylation
S2007 Phosphorylation
S2049 Phosphorylation
S2050 Phosphorylation
R2055 Methylation
S2109 Phosphorylation
Y2140 Phosphorylation
S2141 Phosphorylation
Y2146 Phosphorylation

Research Backgrounds

Function:

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activator for RHOA. Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA.

Subcellular Location:

Cytoplasm>Cell cortex. Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton.
Note: In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in peripheral blood leukocytes (at protein level). Expressed predominantly in peripheral blood leukocytes and at lower levels, in thymus, spleen, testis, prostate, ovary, brain, small intestine and lung.

Subunit Structure:

Interacts (via IQ domains) with CALM. Interacts with RHOA. Interacts (via Rho-GAP domain) with ROBO1; this inhibits the interaction with RHOA and the stimulation of RHOA GTPase activity, and thereby increases the levels of active RHOA.

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

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