MYH15 Antibody | Affinity Biosciences

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Western blot analysis of extracts from K562 cells, using MYH15 antibody.

Product:	MYH15 Antibody
Catalog:	DF4197
Description:	Rabbit polyclonal antibody to MYH15
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Rabbit, Chicken, Xenopus
Mol.Wt.:	225 KD; 225kD(Calculated).
Uniprot:	Q9Y2K3
RRID:	AB_2836406

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(100%), Rabbit(89%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

MYH15 Antibody detects endogenous levels of total MYH15.

RRID:

AB_2836406
Cite Format: Affinity Biosciences Cat# DF4197, RRID:AB_2836406.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

KIAA1000; MYH15; MYH15_HUMAN; Myosin 15; Myosin heavy chain 15; Myosin heavy polypeptide 15; Myosin, heavy chain 15; Myosin-15; Myosin15;

Immunogens

Immunogen:

Uniprot:

Q9Y2K3(MYH15_HUMAN) >>Visit HPA database.

Gene(ID):

MYH15(22989)

Sequence:

Q9Y2K3 MYH15_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVESCLLTFRAFFWWIALIKMDLSDLGEAAAFLRRSEAELLLLQATALDGKKKCWIPDGENAYIEAEVKGSEDDGTVIVETADGESLSIKEDKIQQMNPPEFEMIEDMAMLTHLNEASVLHTLKRRYGQWMIYTYSGLFCVTINPYKWLPVYQKEVMAAYKGKRRSEAPPHIFAVANNAFQDMLHNRENQSILFTGESGAGKTVNSKHIIQYFATIAAMIESRKKQGALEDQIMQANTILEAFGNAKTLRNDNSSRFGKFIRMHFGARGMLSSVDIDIYLLEKSRVIFQQAGERNYHIFYQILSGQKELHDLLLVSANPSDFHFCSCGAVTVESLDDAEELLATEQAMDILGFLPDEKYGCYKLTGAIMHFGNMKFKQKPREEQLEADGTENADKAAFLMGINSSELVKCLIHPRIKVGNEYVTRGQTIEQVTCAVGALSKSMYERMFKWLVARINRALDAKLSRQFFIGILDITGFEILEYNSLEQLCINFTNEKLQQFFNWHMFVLEQEEYKKESIEWVSIGFGLDLQACIDLIEKPMGILSILEEECMFPKATDLTFKTKLFDNHFGKSVHLQKPKPDKKKFEAHFELVHYAGVVPYNISGWLEKNKDLLNETVVAVFQKSSNRLLASLFENYMSTDSAIPFGEKKRKKGASFQTVASLHKENLNKLMTNLKSTAPHFVRCINPNVNKIPGILDPYLVLQQLRCNGVLEGTRICREGFPNRLQYADFKQRYCILNPRTFPKSKFVSSRKAAEELLGSLEIDHTQYRFGITKVFFKAGFLGQLEAIRDERLSKVFTLFQARAQGKLMRIKFQKILEERDALILIQWNIRAFMAVKNWPWMRLFFKIKPLVKSSEVGEEVAGLKEECAQLQKALEKSEFQREELKAKQVSLTQEKNDLILQLQAEQETLANVEEQCEWLIKSKIQLEARVKELSERVEEEEEINSELTARGRKLEDECFELKKEIDDLETMLVKSEKEKRTTEHKVKNLTEEVEFLNEDISKLNRAAKVVQEAHQQTLDDLHMEEEKLSSLSKANLKLEQQVDELEGALEQERKARMNCERELHKLEGNLKLNRESMENLESSQRHLAEELRKKELELSQMNSKVENEKGLVAQLQKTVKELQTQIKDLKEKLEAERTTRAKMERERADLTQDLADLNERLEEVGGSSLAQLEITKKQETKFQKLHRDMEEATLHFETTSASLKKRHADSLAELEGQVENLQQVKQKLEKDKSDLQLEVDDLLTRVEQMTRAKANAEKLCTLYEERLHEATAKLDKVTQLANDLAAQKTKLWSESGEFLRRLEEKEALINQLSREKSNFTRQIEDLRGQLEKETKSQSALAHALQKAQRDCDLLREQYEEEQEVKAELHRTLSKVNAEMVQWRMKYENNVIQRTEDLEDAKKELAIRLQEAAEAMGVANARNASLERARHQLQLELGDALSDLGKVRSAAARLDQKQLQSGKALADWKQKHEESQALLDASQKEVQALSTELLKLKNTYEESIVGQETLRRENKNLQEEISNLTNQVREGTKNLTEMEKVKKLIEEEKTEVQVTLEETEGALERNESKILHFQLELLEAKAELERKLSEKDEEIENFRRKQQCTIDSLQSSLDSEAKSRIEVTRLKKKMEEDLNEMELQLSCANRQVSEATKSLGQLQIQIKDLQMQLDDSTQLNSDLKEQVAVAERRNSLLQSELEDLRSLQEQTERGRRLSEEELLEATERINLFYTQNTSLLSQKKKLEADVARMQKEAEEVVQECQNAEEKAKKAAIEAANLSEELKKKQDTIAHLERTRENMEQTITDLQKRLAEAEQMALMGSRKQIQKLESRVRELEGELEGEIRRSAEAQRGARRLERCIKELTYQAEEDKKNLSRMQTQMDKLQLKVQNYKQQVEVAETQANQYLSKYKKQQHELNEVKERAEVAESQVNKLKIKAREFGKKVQEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Xenopus

100

Chicken

100

Rabbit

Dog

Horse

Sheep

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2K3 As Substrate

Q9Y2K3

Site	PTM Type	Source
S71	Phosphorylation	Uniprot
T81	Phosphorylation	Uniprot
S86	Phosphorylation	Uniprot
S88	Phosphorylation	Uniprot
S191	Phosphorylation	Uniprot
K259	Ubiquitination	Uniprot
K283	Ubiquitination	Uniprot
K375	Acetylation	Uniprot
K514	Acetylation	Uniprot
Y727	Phosphorylation	Uniprot
K731	Ubiquitination	Uniprot
S1100	Phosphorylation	Uniprot
T1125	Phosphorylation	Uniprot
T1532	Phosphorylation	Uniprot
K1540	Ubiquitination	Uniprot
K1543	Ubiquitination	Uniprot
S1615	Phosphorylation	Uniprot
S1619	Phosphorylation	Uniprot
S1691	Phosphorylation	Uniprot
S1695	Phosphorylation	Uniprot
S1714	Phosphorylation	Uniprot
Y1729	Phosphorylation	Uniprot
T1730	Phosphorylation	Uniprot
T1733	Phosphorylation	Uniprot
S1734	Phosphorylation	Uniprot
K1807	Ubiquitination	Uniprot
Y1864	Phosphorylation	Uniprot
Y1890	Phosphorylation	Uniprot

Research Backgrounds

Q9Y2K3_MYH15_HUMAN

Function:

Muscle contraction.

Subcellular Location:

Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.

Subunit Structure:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

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MYH15 Antibody - #DF4197