MYH14 Antibody - #DF4196

(2)  
Product: MYH14 Antibody
Catalog: DF4196
Description: Rabbit polyclonal antibody to MYH14
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 228 KD; 228kD(Calculated).
Uniprot: Q7Z406
RRID: AB_2836405

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(90%), Bovine(90%), Horse(91%), Sheep(89%), Dog(100%)
Clonality:
Polyclonal
Specificity:
MYH14 Antibody detects endogenous levels of total MYH14.
RRID:
AB_2836405
Cite Format: Affinity Biosciences Cat# DF4196, RRID:AB_2836405.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2400004E04Rik; DFNA4; DKFZp667A1311; FLJ13881; FLJ43092; FP17425; II C; KIAA2034; MHC16; Myh 14; MYH14; MYH14_HUMAN; Myosin 14; Myosin; Myosin heavy chain 14; Myosin heavy chain; Myosin heavy chain non muscle IIc; Myosin heavy polypeptide 14; Myosin-14; NMHC II C; NMHC II-C; Non muscle myosin heavy chain IIc; non-muscle IIc; Non-muscle myosin heavy chain IIc; Nonmuscle myosin heavy chain II C; OTTMUSP00000019210;

Immunogens

Immunogen:
Uniprot:

Q7Z406(MYH14_HUMAN) >>Visit HPA database.

Gene(ID):
MYH14(79784)
Expression:
Q7Z406 MYH14_HUMAN:

High levels of expression are found in brain (highest in corpus callosum), heart, kidney, liver, lung, small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No detectable expression is found in thymus, spleen, placenta and lymphocytes.

Sequence:
MAAVTMSVPGRKAPPRPGPVPEAAQPFLFTPRGPSAGGGPGSGTSPQVEWTARRLVWVPSELHGFEAAALRDEGEEEAEVELAESGRRLRLPRDQIQRMNPPKFSKAEDMAELTCLNEASVLHNLRERYYSGLIYTYSGLFCVVINPYKQLPIYTEAIVEMYRGKKRHEVPPHVYAVTEGAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAHVASSPKGRKEPGVPGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVAGYIVGANIETYLLEKSRAIRQAKDECSFHIFYQLLGGAGEQLKADLLLEPCSHYRFLTNGPSSSPGQERELFQETLESLRVLGFSHEEIISMLRMVSAVLQFGNIALKRERNTDQATMPDNTAAQKLCRLLGLGVTDFSRALLTPRIKVGRDYVQKAQTKEQADFALEALAKATYERLFRWLVLRLNRALDRSPRQGASFLGILDIAGFEIFQLNSFEQLCINYTNEKLQQLFNHTMFVLEQEEYQREGIPWTFLDFGLDLQPCIDLIERPANPPGLLALLDEECWFPKATDKSFVEKVAQEQGGHPKFQRPRHLRDQADFSVLHYAGKVDYKANEWLMKNMDPLNDNVAALLHQSTDRLTAEIWKDVEGIVGLEQVSSLGDGPPGGRPRRGMFRTVGQLYKESLSRLMATLSNTNPSFVRCIVPNHEKRAGKLEPRLVLDQLRCNGVLEGIRICRQGFPNRILFQEFRQRYEILTPNAIPKGFMDGKQACEKMIQALELDPNLYRVGQSKIFFRAGVLAQLEEERDLKVTDIIVSFQAAARGYLARRAFQKRQQQQSALRVMQRNCAAYLKLRHWQWWRLFTKVKPLLQVTRQDEVLQARAQELQKVQELQQQSAREVGELQGRVAQLEEERARLAEQLRAEAELCAEAEETRGRLAARKQELELVVSELEARVGEEEECSRQMQTEKKRLQQHIQELEAHLEAEEGARQKLQLEKVTTEAKMKKFEEDLLLLEDQNSKLSKERKLLEDRLAEFSSQAAEEEEKVKSLNKLRLKYEATIADMEDRLRKEEKGRQELEKLKRRLDGESSELQEQMVEQQQRAEELRAQLGRKEEELQAALARAEDEGGARAQLLKSLREAQAALAEAQEDLESERVARTKAEKQRRDLGEELEALRGELEDTLDSTNAQQELRSKREQEVTELKKTLEEETRIHEAAVQELRQRHGQALGELAEQLEQARRGKGAWEKTRLALEAEVSELRAELSSLQTARQEGEQRRRRLELQLQEVQGRAGDGERARAEAAEKLQRAQAELENVSGALNEAESKTIRLSKELSSTEAQLHDAQELLQEETRAKLALGSRVRAMEAEAAGLREQLEEEAAARERAGRELQTAQAQLSEWRRRQEEEAGALEAGEEARRRAAREAEALTQRLAEKTETVDRLERGRRRLQQELDDATMDLEQQRQLVSTLEKKQRKFDQLLAEEKAAVLRAVEERERAEAEGREREARALSLTRALEEEQEAREELERQNRALRAELEALLSSKDDVGKSVHELERACRVAEQAANDLRAQVTELEDELTAAEDAKLRLEVTVQALKTQHERDLQGRDEAGEERRRQLAKQLRDAEVERDEERKQRTLAVAARKKLEGELEELKAQMASAGQGKEEAVKQLRKMQAQMKELWREVEETRTSREEIFSQNRESEKRLKGLEAEVLRLQEELAASDRARRQAQQDRDEMADEVANGNLSKAAILEEKRQLEGRLGQLEEELEEEQSNSELLNDRYRKLLLQVESLTTELSAERSFSAKAESGRQQLERQIQELRGRLGEEDAGARARHKMTIAALESKLAQAEEQLEQETRERILSGKLVRRAEKRLKEVVLQVEEERRVADQLRDQLEKGNLRVKQLKRQLEEAEEEASRAQAGRRRLQRELEDVTESAESMNREVTTLRNRLRRGPLTFTTRTVRQVFRLEEGVASDEEAEEAQPGSGPSPEPEGSPPAHPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Horse
91
Pig
90
Bovine
90
Sheep
89
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z406 As Substrate

Site PTM Type Enzyme
T30 Phosphorylation
R32 Methylation
S35 Phosphorylation
S42 Phosphorylation
T44 Phosphorylation
S45 Phosphorylation
T51 Phosphorylation
S60 Phosphorylation
K103 Ubiquitination
S105 Phosphorylation
Y137 Phosphorylation
Y154 Phosphorylation
Y162 Phosphorylation
S193 Phosphorylation
K204 Ubiquitination
Y214 Phosphorylation
S220 Phosphorylation
S221 Phosphorylation
K252 Ubiquitination
T253 Phosphorylation
K255 Ubiquitination
K264 Ubiquitination
K288 Ubiquitination
S335 Phosphorylation
T395 Phosphorylation
S412 Phosphorylation
Y426 Phosphorylation
K429 Acetylation
K429 Ubiquitination
K433 Ubiquitination
K566 Ubiquitination
S567 Phosphorylation
S595 Phosphorylation
Y674 Phosphorylation
K675 Methylation
S677 Phosphorylation
S686 Phosphorylation
S691 Phosphorylation
T749 Phosphorylation
Y778 Phosphorylation
S783 Phosphorylation
T804 Phosphorylation
S809 Phosphorylation
Y843 Phosphorylation
K845 Acetylation
K845 Ubiquitination
K859 Ubiquitination
K880 Ubiquitination
S942 Phosphorylation
K985 Ubiquitination
R1024 Methylation
S1041 Phosphorylation
K1105 Acetylation
K1105 Ubiquitination
S1146 Phosphorylation
T1175 Phosphorylation
T1194 Phosphorylation
T1204 Phosphorylation
S1328 Phosphorylation
T1422 Phosphorylation
T1462 Phosphorylation
K1469 Acetylation
K1469 Methylation
K1469 Ubiquitination
S1535 Phosphorylation
T1573 Phosphorylation
T1585 Phosphorylation
T1591 Phosphorylation
T1630 Phosphorylation
S1652 Phosphorylation
K1657 Methylation
R1665 Methylation
S1767 Phosphorylation
S1769 Phosphorylation
K1799 Acetylation
T1940 Phosphorylation
R1947 Methylation
T1951 Phosphorylation
S1969 Phosphorylation
S1980 Phosphorylation
S1983 Phosphorylation
S1989 Phosphorylation

Research Backgrounds

Function:

Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.

Tissue Specificity:

High levels of expression are found in brain (highest in corpus callosum), heart, kidney, liver, lung, small intestine, colon and skeletal muscle. Expression is low in organs composed mainly of smooth muscle, such as aorta, uterus and urinary bladder. No detectable expression is found in thymus, spleen, placenta and lymphocytes.

Subunit Structure:

Myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.