Product: GNL3L Antibody
Catalog: DF4113
Description: Rabbit polyclonal antibody to GNL3L
Application: WB IF/ICC
Reactivity: Human, Monkey
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 66 KD; 66kD(Calculated).
Uniprot: Q9NVN8
RRID: AB_2836404

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(91%), Bovine(100%), Horse(91%), Rabbit(82%), Dog(82%)
Clonality:
Polyclonal
Specificity:
GNL3L Antibody detects endogenous levels of total GNL3L.
RRID:
AB_2836404
Cite Format: Affinity Biosciences Cat# DF4113, RRID:AB_2836404.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ10613; Guanine nucleotide binding protein-like 3 (nucleolar)-like; novel GTPase; OTTHUMP00000023374;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MMKLRHKNKKPGEGSKGHKKISWPYPQPAKQNGKKATSKVPSAPHFVHPNDHANREAELKKKWVEEMREKQQAAREQERQKRRTIESYCQDVLRRQEEFEHKEEVLQELNMFPQLDDEATRKAYYKEFRKVVEYSDVILEVLDARDPLGCRCFQMEEAVLRAQGNKKLVLVLNKIDLVPKEVVEKWLDYLRNELPTVAFKASTQHQVKNLNRCSVPVDQASESLLKSKACFGAENLMRVLGNYCRLGEVRTHIRVGVVGLPNVGKSSLINSLKRSRACSVGAVPGITKFMQEVYLDKFIRLLDAPGIVPGPNSEVGTILRNCVHVQKLADPVTPVETILQRCNLEEISNYYGVSGFQTTEHFLTAVAHRLGKKKKGGLYSQEQAAKAVLADWVSGKISFYIPPPATHTLPTHLSAEIVKEMTEVFDIEDTEQANEDTMECLATGESDELLGDTDPLEMEIKLLHSPMTKIADAIENKTTVYKIGDLTGYCTNPNRHQMGWAKRNVDHRPKSNSMVDVCSVDRRSVLQRIMETDPLQQGQALASALKNKKKMQKRADKIASKLSDSMMSALDLSGNADDGVGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Pig
91
Horse
91
Dog
82
Rabbit
82
Xenopus
70
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NVN8 As Substrate

Site PTM Type Enzyme
K20 Sumoylation
S22 Phosphorylation
K39 Ubiquitination
S42 Phosphorylation
K60 Ubiquitination
K62 Sumoylation
K62 Ubiquitination
K70 Ubiquitination
S87 Phosphorylation
K126 Ubiquitination
K167 Ubiquitination
K174 Ubiquitination
K180 Ubiquitination
K185 Ubiquitination
K200 Methylation
K200 Ubiquitination
K208 Ubiquitination
S214 Phosphorylation
K226 Ubiquitination
K228 Ubiquitination
K265 Ubiquitination
K273 Ubiquitination
K288 Ubiquitination
Y294 Phosphorylation
K297 Ubiquitination
K327 Ubiquitination
T333 Phosphorylation
S348 Phosphorylation
K375 Sumoylation
K375 Ubiquitination
K386 Ubiquitination
S465 Phosphorylation
K469 Ubiquitination
K477 Sumoylation
K477 Ubiquitination
K482 Sumoylation
K482 Ubiquitination
Y489 Phosphorylation
K502 Acetylation
K502 Ubiquitination
S513 Phosphorylation
S519 Phosphorylation
S543 Phosphorylation
K546 Ubiquitination
S573 Phosphorylation

Research Backgrounds

Function:

Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP.

Subcellular Location:

Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with MDM2; this interaction, which occurs in the nucleoplasm, stabilizes MDM2. Indirectly interacts with TP53, via MDM2-binding. Interacts with TERF1; this interaction probably occurs in the nucleoplasm and is increased during mitosis, when the nucleolus is disassembled. This binding may promote TERF1 homodimerization. Interacts with TERT.

Family&Domains:

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Belongs to the TRAFAC class YlqF/YawG GTPase family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

References

1). GNL3L promotes autophagy via regulating AMPK signaling in esophageal cancer cells. Medical oncology (Northwood, London, England), 2023 (PubMed: 38148364) [IF=3.4]

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