GNL3L Antibody | Affinity Biosciences

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Western blot analysis of extracts from COS-7, using GNL3L Antibody.

Product:	GNL3L Antibody
Catalog:	DF4113
Description:	Rabbit polyclonal antibody to GNL3L
Application:	WB IF/ICC
Reactivity:	Human, Monkey
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	66 KD; 66kD(Calculated).
Uniprot:	Q9NVN8
RRID:	AB_2836404

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Pig(91%), Bovine(100%), Horse(91%), Rabbit(82%), Dog(82%)

Clonality:

Polyclonal

Specificity:

GNL3L Antibody detects endogenous levels of total GNL3L.

RRID:

AB_2836404
Cite Format: Affinity Biosciences Cat# DF4113, RRID:AB_2836404.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ10613; Guanine nucleotide binding protein-like 3 (nucleolar)-like; novel GTPase; OTTHUMP00000023374;

Immunogens

Immunogen:

Uniprot:

Q9NVN8(GNL3L_HUMAN) >>Visit HPA database.

Gene(ID):

GNL3L(54552)

Sequence:

Q9NVN8 GNL3L_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMKLRHKNKKPGEGSKGHKKISWPYPQPAKQNGKKATSKVPSAPHFVHPNDHANREAELKKKWVEEMREKQQAAREQERQKRRTIESYCQDVLRRQEEFEHKEEVLQELNMFPQLDDEATRKAYYKEFRKVVEYSDVILEVLDARDPLGCRCFQMEEAVLRAQGNKKLVLVLNKIDLVPKEVVEKWLDYLRNELPTVAFKASTQHQVKNLNRCSVPVDQASESLLKSKACFGAENLMRVLGNYCRLGEVRTHIRVGVVGLPNVGKSSLINSLKRSRACSVGAVPGITKFMQEVYLDKFIRLLDAPGIVPGPNSEVGTILRNCVHVQKLADPVTPVETILQRCNLEEISNYYGVSGFQTTEHFLTAVAHRLGKKKKGGLYSQEQAAKAVLADWVSGKISFYIPPPATHTLPTHLSAEIVKEMTEVFDIEDTEQANEDTMECLATGESDELLGDTDPLEMEIKLLHSPMTKIADAIENKTTVYKIGDLTGYCTNPNRHQMGWAKRNVDHRPKSNSMVDVCSVDRRSVLQRIMETDPLQQGQALASALKNKKKMQKRADKIASKLSDSMMSALDLSGNADDGVGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Pig

Horse

Dog

Rabbit

Xenopus

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NVN8 As Substrate

Q9NVN8

Site	PTM Type	Source
K20	Sumoylation	Uniprot
S22	Phosphorylation	Uniprot
K39	Ubiquitination	Uniprot
S42	Phosphorylation	Uniprot
K60	Ubiquitination	Uniprot
K62	Sumoylation	Uniprot
K62	Ubiquitination	Uniprot
K70	Ubiquitination	Uniprot
S87	Phosphorylation	Uniprot
K126	Ubiquitination	Uniprot
K167	Ubiquitination	Uniprot
K174	Ubiquitination	Uniprot
K180	Ubiquitination	Uniprot
K185	Ubiquitination	Uniprot
K200	Methylation	Uniprot
K200	Ubiquitination	Uniprot
K208	Ubiquitination	Uniprot
S214	Phosphorylation	Uniprot
K226	Ubiquitination	Uniprot
K228	Ubiquitination	Uniprot
K265	Ubiquitination	Uniprot
K273	Ubiquitination	Uniprot
K288	Ubiquitination	Uniprot
Y294	Phosphorylation	Uniprot
K297	Ubiquitination	Uniprot
K327	Ubiquitination	Uniprot
T333	Phosphorylation	Uniprot
S348	Phosphorylation	Uniprot
K375	Sumoylation	Uniprot
K375	Ubiquitination	Uniprot
K386	Ubiquitination	Uniprot
S465	Phosphorylation	Uniprot
K469	Ubiquitination	Uniprot
K477	Sumoylation	Uniprot
K477	Ubiquitination	Uniprot
K482	Sumoylation	Uniprot
K482	Ubiquitination	Uniprot
Y489	Phosphorylation	Uniprot
K502	Acetylation	Uniprot
K502	Ubiquitination	Uniprot
S513	Phosphorylation	Uniprot
S519	Phosphorylation	Uniprot
S543	Phosphorylation	Uniprot
K546	Ubiquitination	Uniprot
S573	Phosphorylation	Uniprot

Research Backgrounds

Q9NVN8_GNL3L_HUMAN

Function:

Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP.

Subcellular Location:

Nucleus>Nucleolus.

Subunit Structure:

Interacts with MDM2; this interaction, which occurs in the nucleoplasm, stabilizes MDM2. Indirectly interacts with TP53, via MDM2-binding. Interacts with TERF1; this interaction probably occurs in the nucleoplasm and is increased during mitosis, when the nucleolus is disassembled. This binding may promote TERF1 homodimerization. Interacts with TERT.

Family&Domains:

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Belongs to the TRAFAC class YlqF/YawG GTPase family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

References

1). GNL3L promotes autophagy via regulating AMPK signaling in esophageal cancer cells. Medical oncology (Northwood, London, England), 2023 (PubMed: 38148364) [IF=3.4]

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GNL3L Antibody - #DF4113