GNAT2 Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from COLO cells, using GNAT2 antibody.

Product:	GNAT2 Antibody
Catalog:	DF4110
Description:	Rabbit polyclonal antibody to GNAT2
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	40 KD; 40kD(Calculated).
Uniprot:	P19087
RRID:	AB_2836401

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

GNAT2 Antibody detects endogenous levels of total GNAT2.

RRID:

AB_2836401
Cite Format: Affinity Biosciences Cat# DF4110, RRID:AB_2836401.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACHM4; Cone type transducin alpha subunit; GNAT 2; GNAT C; Gnat2; GNAT2_HUMAN; GNATC; Guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 2; Guanine nucleotide binding protein G t subunit alpha 2; Guanine nucleotide-binding protein G(t) subunit alpha-2; Transducin alpha 2; Transducin alpha-2 chain; Transducin alpha2; Transducin cone specific alpha polypeptide;

Immunogens

Immunogen:

Uniprot:

P19087(GNAT2_HUMAN) >>Visit HPA database.

Gene(ID):

GNAT2(2780)

Expression:

P19087 GNAT2_HUMAN:

Retinal rod outer segment.

Sequence:

P19087 GNAT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSGASAEDKELAKRSKELEKKLQEDADKEAKTVKLLLLGAGESGKSTIVKQMKIIHQDGYSPEECLEFKAIIYGNVLQSILAIIRAMTTLGIDYAEPSCADDGRQLNNLADSIEEGTMPPELVEVIRRLWKDGGVQACFERAAEYQLNDSASYYLNQLERITDPEYLPSEQDVLRSRVKTTGIIETKFSVKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFCAALSAYDMVLVEDDEVNRMHESLHLFNSICNHKFFAATSIVLFLNKKDLFEEKIKKVHLSICFPEYDGNNSYDDAGNYIKSQFLDLNMRKDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Zebrafish

Xenopus

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19087 As Substrate

P19087

Site	PTM Type	Source
S3	Phosphorylation	Uniprot
S6	Phosphorylation	Uniprot
K17	Acetylation	Uniprot
K21	Acetylation	Uniprot
K35	Acetylation	Uniprot
S44	Phosphorylation	Uniprot
K46	Ubiquitination	Uniprot
K51	Ubiquitination	Uniprot
Y95	Phosphorylation	Uniprot
K192	Ubiquitination	Uniprot
S206	Phosphorylation	Uniprot
K271	Ubiquitination	Uniprot

Research Backgrounds

P19087_GNAT2_HUMAN

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Transducin is an amplifier and one of the transducers of a visual impulse that performs the coupling between rhodopsin and cGMP-phosphodiesterase.

Subcellular Location:

Cell projection>Cilium>Photoreceptor outer segment. Photoreceptor inner segment.
Note: Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light.

Tissue Specificity:

Retinal rod outer segment.

Subunit Structure:

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Family&Domains:

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Research Fields

· Organismal Systems > Sensory system > Phototransduction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

GNAT2 Antibody - #DF4110