GNAT1 Antibody - #DF4109

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Product: GNAT1 Antibody
Catalog: DF4109
Description: Rabbit polyclonal antibody to GNAT1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 36 KD; 40kD(Calculated).
Uniprot: P11488
RRID: AB_2836400

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
GNAT1 Antibody detects endogenous levels of total GNAT1.
RRID:
AB_2836400
Cite Format: Affinity Biosciences Cat# DF4109, RRID:AB_2836400.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CSNBAD3; GBT1; GNAT1; GNAT1_HUMAN; GNATR; guanine nucleotide binding protein (G protein) alpha transducing activity polypeptide 1; guanine nucleotide binding protein G(T) alpha 1 subunit; Guanine nucleotide-binding protein G(t) subunit alpha-1; Rod specific transducin; rod-type transducin alpha subunit; Transducin alpha-1 chain; transducin, rod-specific;

Immunogens

Immunogen:
Uniprot:

P11488(GNAT1_HUMAN) >>Visit HPA database.

Gene(ID):
GNAT1(2779)
Expression:
P11488 GNAT1_HUMAN:

Rod photoreceptor cells (PubMed:1614872). Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium (PubMed:22190596).

Sequence:
MGAGASAEEKHSRELEKKLKEDAEKDARTVKLLLLGAGESGKSTIVKQMKIIHQDGYSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFERASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTTGIIETQFSFKDLNFRMFDVGGQRSERKKWIHCFEGVTCIIFIAALSAYDMVLVEDDEVNRMHESLHLFNSICNHRYFATTSIVLFLNKKDVFFEKIKKAHLSICFPDYDGPNTYEDAGNYIKVQFLELNMRRDVKEIYSHMTCATDTQNVKFVFDAVTDIIIKENLKDCGLF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
82
Zebrafish
82
Pig
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11488 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
K31 Acetylation
S40 Phosphorylation
K42 Ubiquitination
K47 Ubiquitination
S130 Phosphorylation
Y142 Phosphorylation
S202 Phosphorylation

Research Backgrounds

Function:

Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina. Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).

Subcellular Location:

Cell projection>Cilium>Photoreceptor outer segment. Membrane>Peripheral membrane protein. Photoreceptor inner segment.
Note: Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Rod photoreceptor cells. Predominantly expressed in the retina followed by the ciliary body, iris and retinal pigment epithelium.

Subunit Structure:

Heterotrimeric G proteins are composed of 3 subunits alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RHO. Interacts with RGS9 and PDE6G (By similarity). Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons.

Family&Domains:

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Research Fields

· Organismal Systems > Sensory system > Phototransduction.

References

1). Frequency cumulative effect of subthreshold energy laser-activated remote phosphors irradiation on visual function in guinea pigs. International Journal of Ophthalmology, 2022 (PubMed: 35186679) [IF=1.4]

Application: IF/ICC    Species: retina    Sample: guinea pigs

Figure 5| High frequency irradiation did not change the density of photoreceptor cells A: Fluorescent staining of biotinylated peanut agglutinin and transducing-alpha in the retina of guinea pigs after high frequency irradiation

Application: IF/ICC    Species: Pig    Sample: photoreceptor cells

Figure 5 High frequency irradiation did not change the density of photoreceptor cells A: Fluorescent staining of biotinylated peanut agglutinin and transducing-alpha in the retina of guinea pigs after high frequency irradiation; B: The density of retinal cone cells of guinea pigs after high frequency irradiation; C: The density of retinal rod cells of guinea pigs after high frequency irradiation. Statistical significance was determined by the paired sample t-test. Data are presented as the mean±SEM. All analyses were performed in duplicate. n=3.

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