GNAL Antibody - #DF4108

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Product: GNAL Antibody
Catalog: DF4108
Description: Rabbit polyclonal antibody to GNAL
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 40 KD; 44kD(Calculated).
Uniprot: P38405
RRID: AB_2836399

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
GNAL Antibody detects endogenous levels of total GNAL.
RRID:
AB_2836399
Cite Format: Affinity Biosciences Cat# DF4108, RRID:AB_2836399.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

;Adenylate cyclase stimulating G alpha protein, olfactory type; Adenylate cyclase-stimulating G alpha protein; DYT25; G alpha olf; Gnal; GNAL_HUMAN; Guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type; Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide, olfactory type; Guanine nucleotide binding protein G(olf) subunit alpha; Guanine nucleotide-binding protein G(olf) subunit alpha; olfactory type;

Immunogens

Immunogen:
Uniprot:

P38405(GNAL_HUMAN) >>Visit HPA database.

Gene(ID):
GNAL(2774)
Expression:
P38405 GNAL_HUMAN:

Detected in olfactory neuroepithelium, brain, testis, and to a lower extent in retina, lung alveoli, spleen. Trace amounts where seen in kidney, adrenal gland and liver. Found to be expressed in all the insulinomas examined.

Sequence:
MGCLGGNSKTTEDQGVDEKERREANKKIEKQLQKERLAYKATHRLLLLGAGESGKSTIVKQMRILHVNGFNPEEKKQKILDIRKNVKDAIVTIVSAMSTIIPPVPLANPENQFRSDYIKSIAPITDFEYSQEFFDHVKKLWDDEGVKACFERSNEYQLIDCAQYFLERIDSVSLVDYTPTDQDLLRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIYVAACSSYNMVIREDNNTNRLRESLDLFESIWNNRWLRTISIILFLNKQDMLAEKVLAGKSKIEDYFPEYANYTVPEDATPDAGEDPKVTRAKFFIRDLFLRISTATGDGKHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLKQYELL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P38405 As Substrate

Site PTM Type Enzyme
K30 Ubiquitination
S53 Phosphorylation
K55 Ubiquitination
K60 Ubiquitination
K138 Ubiquitination
K139 Ubiquitination
K203 Ubiquitination
S256 Phosphorylation
K292 Ubiquitination
K294 Ubiquitination
K320 Ubiquitination
Y345 Phosphorylation
Y347 Phosphorylation

Research Backgrounds

Function:

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(olf) alpha mediates signal transduction within the olfactory neuroepithelium and the basal ganglia. May be involved in some aspect of visual transduction, and in mediating the effect of one or more hormones/neurotransmitters.

Tissue Specificity:

Detected in olfactory neuroepithelium, brain, testis, and to a lower extent in retina, lung alveoli, spleen. Trace amounts where seen in kidney, adrenal gland and liver. Found to be expressed in all the insulinomas examined.

Subunit Structure:

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GAS2L2.

Family&Domains:

Belongs to the G-alpha family. G(s) subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

References

1). Long Mu Qing Xin mixture improves behavioral performance in spontaneously hypertensive rats (SHR/NCrl) by upregulating catecholamine neurotransmitters in prefrontal cortex and striatum via DRD1/cAMP/PKA-CREB signaling pathway. Frontiers in pharmacology, 2024 (PubMed: 39027341) [IF=5.6]

Application: WB    Species: Rat    Sample:

FIGURE 6 Effects of LMQXM on the relative expression of DRD1, Gαs, Gαolf, PKA, p-CREB, and BDNF proteins in rat PFC and striatum. (A) Relative expression of DRD1 proteins; (B) Relative expression of Gαs proteins; (C) Relative expression of Gαolf proteins; (D) Relative expression of PKA proteins; (E) Relative expression of p-CREB proteins; (F) Relative expression of BDNF proteins. (H) Western blot analysis images of each group of proteins in PFC; (G) Western blot analysis images of each group of proteins in striatum. Data are expressed as mean ± standard deviation, n = 3 for each group. Compared with the WKY group, # p < 0.05, ## p < 0.01, ### p < 0.001, compared with the SHR group, * p < 0.05, ** p < 0.01, *** p < 0.001 and compared with the LMQXM-MD group, ^ p < 0.05.

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