NEIL3 Antibody - #DF4042
| Product: | NEIL3 Antibody |
| Catalog: | DF4042 |
| Description: | Rabbit polyclonal antibody to NEIL3 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 68 KD; 68kD(Calculated). |
| Uniprot: | Q8TAT5 |
| RRID: | AB_2836395 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4042, RRID:AB_2836395.
Fold/Unfold
123; AI449477; BC034753; C85903; DNA glycosylase FPG2; DNA glycosylase hFPG2; DNA glycosylase/AP lyase Neil3; EC 3.2.2.-; EC 4.2.99.18; Endonuclease 8-like 3; Endonuclease VIII-like 3; FGP2; FLJ10858; FPG2; hFPG2; hNEI3; MGC27861; MGC36916; Nei endonuclease VIII-like 3 (E. coli); nei endonuclease VIII-like 3; Nei like 3 (E. coli); Nei-like protein 3; NEIL3; NEIL3_HUMAN; OTTHUMP00000218810; RGD1310562;
Immunogens
A synthesized peptide derived from human NEIL3, corresponding to a region within C-terminal amino acids.
Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.
- Q8TAT5 NEIL3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVEGPGCTLNGEKIRARVLPGQAVTGVRGSALRSLQGRALRLAASTVVVSPQAAALNNDSSQNVLSLFNGYVYSGVETLGKELFMYFGPKALRIHFGMKGFIMINPLEYKYKNGASPVLEVQLTKDLICFFDSSVELRNSMESQQRIRMMKELDVCSPEFSFLRAESEVKKQKGRMLGDVLMDQNVLPGVGNIIKNEALFDSGLHPAVKVCQLTDEQIHHLMKMIRDFSILFYRCRKAGLALSKHYKVYKRPNCGQCHCRITVCRFGDNNRMTYFCPHCQKENPQHVDICKLPTRNTIISWTSSRVDHVMDSVARKSEEHWTCVVCTLINKPSSKACDACLTSRPIDSVLKSEENSTVFSHLMKYPCNTFGKPHTEVKINRKTAFGTTTLVLTDFSNKSSTLERKTKQNQILDEEFQNSPPASVCLNDIQHPSKKTTNDITQPSSKVNISPTISSESKLFSPAHKKPKTAQYSSPELKSCNPGYSNSELQINMTDGPRTLNPDSPRCSKHNRLCILRVVGKDGENKGRQFYACPLPREAQCGFFEWADLSFPFCNHGKRSTMKTVLKIGPNNGKNFFVCPLGKEKQCNFFQWAENGPGIKIIPGC
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TAT5 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K13 | Ubiquitination | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K195 | Sumoylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K244 | Acetylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| Y246 | Phosphorylation | Uniprot | |
| K247 | Acetylation | Uniprot | |
| K250 | Acetylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K316 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| K458 | Sumoylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| T564 | Phosphorylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| K574 | Ubiquitination | Uniprot | |
| K583 | Ubiquitination | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot |
Research Backgrounds
DNA glycosylase which prefers single-stranded DNA (ssDNA), or partially ssDNA structures such as bubble and fork structures, to double-stranded DNA (dsDNA). In vitro, displays strong glycosylase activity towards the hydantoin lesions spiroiminodihydantoin (Sp) and guanidinohydantoin (Gh) in both ssDNA and dsDNA; also recognizes FapyA, FapyG, 5-OHU, 5-OHC, 5-OHMH, Tg and 8-oxoA lesions in ssDNA. No activity on 8-oxoG detected. Also shows weak DNA-(apurinic or apyrimidinic site) lyase activity. In vivo, appears to be the primary enzyme involved in removing Sp and Gh from ssDNA in neonatal tissues. Seems to be an important facilitator of cell proliferation in certain populations, for example neural stem/progenitor cells and tumor cells, suggesting a role in replication-associated DNA repair.
Nucleus.
Expressed in keratinocytes and embryonic fibroblasts (at protein level). Also detected in thymus, testis and fetal lung primary fibroblasts.
The N-terminal region (2-281) contains the glycosylase and lyase activities.
Belongs to the FPG family.
Research Fields
· Genetic Information Processing > Replication and repair > Base excision repair.
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