ENTPD1/CD39 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of extracts from HeLa cells using ENTPD1 antibody.

Figure 7 The expression of CD39, CD103, and CD8 were tested by immunofluorescence of MC38 tumors (n=3, Scale bar=1000 μm).

Product:	ENTPD1/CD39 Antibody
Catalog:	DF4031
Description:	Rabbit polyclonal antibody to ENTPD1/CD39
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	65 KD; 58kD(Calculated).
Uniprot:	P49961
RRID:	AB_2836391

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

ENTPD1/CD39 Antibody detects endogenous levels of total ENTPD1/CD39.

RRID:

AB_2836391
Cite Format: Affinity Biosciences Cat# DF4031, RRID:AB_2836391.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATPDase; CD 39; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto apyrase; Ecto ATP diphosphohydrolase; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; ENTP1_HUMAN; ENTPD 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase1; SPG64;

Immunogens

Immunogen:

Uniprot:

P49961(ENTP1_HUMAN) >>Visit HPA database.

Gene(ID):

ENTPD1(953)

Expression:

P49961 ENTP1_HUMAN:

Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only.

Sequence:

P49961 ENTP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEDTKESNVKTFCSKNILAILGFSSIIAVIALLAVGLTQNKALPENVKYGIVLDAGSSHTSLYIYKWPAEKENDTGVVHQVEECRVKGPGISKFVQKVNEIGIYLTDCMERAREVIPRSQHQETPVYLGATAGMRLLRMESEELADRVLDVVERSLSNYPFDFQGARIITGQEEGAYGWITINYLLGKFSQKTRWFSIVPYETNNQETFGALDLGGASTQVTFVPQNQTIESPDNALQFRLYGKDYNVYTHSFLCYGKDQALWQKLAKDIQVASNEILRDPCFHPGYKKVVNVSDLYKTPCTKRFEMTLPFQQFEIQGIGNYQQCHQSILELFNTSYCPYSQCAFNGIFLPPLQGDFGAFSAFYFVMKFLNLTSEKVSQEKVTEMMKKFCAQPWEEIKTSYAGVKEKYLSEYCFSGTYILSLLLQGYHFTADSWEHIHFIGKIQGSDAGWTLGYMLNLTNMIPAEQPLSTPLSHSTYVFLMVLFSLVLFTVAIIGLLIFHKPSYFWKDMV

PTMs - P49961 As Substrate

P49961

Site	PTM Type	Source
Y49	Phosphorylation	Uniprot
Y65	Phosphorylation	Uniprot
N73	N-Glycosylation	Uniprot
N292	N-Glycosylation	Uniprot

Research Backgrounds

P49961_ENTP1_HUMAN

Function:

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.

PTMs:

The N-terminus is blocked.

Palmitoylated in the N-terminal part.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Tissue Specificity:

Subunit Structure:

Homodimer; disulfide-linked.

Family&Domains:

Belongs to the GDA1/CD39 NTPase family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

References

1). Kaempferol alleviates calcium oxalate crystal-induced renal injury and crystal deposition via regulation of the AR/NOX2 signaling pathway. PHYTOMEDICINE, 2021 (PubMed: 33852977) [IF=7.9]

2). A preclinical study: correlation between PD-L1 PET imaging and the prediction of therapy efficacy of MC38 tumor with 68Ga-labeled PD-L1 targeted nanobody. Aging-US, 2021 (PubMed: 33910164) [IF=5.2]

Application: IF/ICC Species: Mice Sample: tumors tissues

Figure 7 The expression of CD39, CD103, and CD8 were tested by immunofluorescence of MC38 tumors (n=3, Scale bar=1000 μm). (A) MC38-hPD-L1 control (B) MC38-hPD-L1 (C) MC38-hPD-L1/KO (D) MC38-KO CD8 was indicated by red signals; CD39 or CD103 was indicated by green signals; nuclei, blue 4’,6-diamidino-2-phenylindole (DAPI) signals. Local amplifies the area indicated by the white box. Scale bar=200 μm.

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ENTPD1/CD39 Antibody - #DF4031