Product: ENTPD1/CD39 Antibody
Catalog: DF4031
Description: Rabbit polyclonal antibody to ENTPD1/CD39
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 65 KD; 58kD(Calculated).
Uniprot: P49961
RRID: AB_2836391

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
ENTPD1/CD39 Antibody detects endogenous levels of total ENTPD1/CD39.
RRID:
AB_2836391
Cite Format: Affinity Biosciences Cat# DF4031, RRID:AB_2836391.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATPDase; CD 39; CD39; CD39 antigen; DKFZp686D194; DKFZp686I093; Ecto apyrase; Ecto ATP diphosphohydrolase; Ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; Ectonucleoside triphosphate diphosphohydrolase 1; ENTP1_HUMAN; ENTPD 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPDase 1; NTPDase1; SPG64;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P49961 ENTP1_HUMAN:

Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only.

Sequence:
MEDTKESNVKTFCSKNILAILGFSSIIAVIALLAVGLTQNKALPENVKYGIVLDAGSSHTSLYIYKWPAEKENDTGVVHQVEECRVKGPGISKFVQKVNEIGIYLTDCMERAREVIPRSQHQETPVYLGATAGMRLLRMESEELADRVLDVVERSLSNYPFDFQGARIITGQEEGAYGWITINYLLGKFSQKTRWFSIVPYETNNQETFGALDLGGASTQVTFVPQNQTIESPDNALQFRLYGKDYNVYTHSFLCYGKDQALWQKLAKDIQVASNEILRDPCFHPGYKKVVNVSDLYKTPCTKRFEMTLPFQQFEIQGIGNYQQCHQSILELFNTSYCPYSQCAFNGIFLPPLQGDFGAFSAFYFVMKFLNLTSEKVSQEKVTEMMKKFCAQPWEEIKTSYAGVKEKYLSEYCFSGTYILSLLLQGYHFTADSWEHIHFIGKIQGSDAGWTLGYMLNLTNMIPAEQPLSTPLSHSTYVFLMVLFSLVLFTVAIIGLLIFHKPSYFWKDMV

PTMs - P49961 As Substrate

Site PTM Type Enzyme
Y49 Phosphorylation
Y65 Phosphorylation
N73 N-Glycosylation
N292 N-Glycosylation

Research Backgrounds

Function:

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.

PTMs:

The N-terminus is blocked.

Palmitoylated in the N-terminal part.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only.

Subunit Structure:

Homodimer; disulfide-linked.

Family&Domains:

Belongs to the GDA1/CD39 NTPase family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

References

1). Kaempferol alleviates calcium oxalate crystal-induced renal injury and crystal deposition via regulation of the AR/NOX2 signaling pathway. PHYTOMEDICINE, 2021 (PubMed: 33852977) [IF=7.9]

2). A preclinical study: correlation between PD-L1 PET imaging and the prediction of therapy efficacy of MC38 tumor with 68Ga-labeled PD-L1 targeted nanobody. Aging-US, 2021 (PubMed: 33910164) [IF=5.2]

Application: IF/ICC    Species: Mice    Sample: tumors tissues

Figure 7 The expression of CD39, CD103, and CD8 were tested by immunofluorescence of MC38 tumors (n=3, Scale bar=1000 μm). (A) MC38-hPD-L1 control (B) MC38-hPD-L1 (C) MC38-hPD-L1/KO (D) MC38-KO CD8 was indicated by red signals; CD39 or CD103 was indicated by green signals; nuclei, blue 4’,6-diamidino-2-phenylindole (DAPI) signals. Local amplifies the area indicated by the white box. Scale bar=200 μm.

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