RNF138 Antibody | Affinity Biosciences

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Product:	RNF138 Antibody
Catalog:	DF4025
Description:	Rabbit polyclonal antibody to RNF138
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	28 KD; 28kD(Calculated).
Uniprot:	Q8WVD3
RRID:	AB_2836385

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

RNF138 Antibody detects endogenous levels of total RNF138.

RRID:

AB_2836385
Cite Format: Affinity Biosciences Cat# DF4025, RRID:AB_2836385.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

E3 ubiquitin protein ligase RNF138; E3 ubiquitin-protein ligase RNF138; hNARF; HSD 4; HSD4; MGC8758; NARF; Nemo like kinase associated RING finger protein; Nemo-like kinase-associated RING finger protein; NLK associated RING finger protein; NLK-associated RING finger protein; RING finger protein 138; ring finger protein 138, E3 ubiquitin protein ligase; RN138_HUMAN; Rnf138; STRIN;

Immunogens

Immunogen:

Uniprot:

Q8WVD3(RN138_HUMAN) >>Visit HPA database.

Gene(ID):

RNF138(51444)

Sequence:

Q8WVD3 RN138_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEDLSAATSYTEDDFYCPVCQEVLKTPVRTTACQHVFCRKCFLTAMRESGAHCPLCRGNVTRRERACPERALDLENIMRKFSGSCRCCAKQIKFYRMRHHYKSCKKYQDEYGVSSIIPNFQISQDSVGNSNRSETSTSDNTETYQENTSSSGHPTFKCPLCQESNFTRQRLLDHCNSNHLFQIVPVTCPICVSLPWGDPSQITRNFVSHLNQRHQFDYGEFVNLQLDEETQYQTAVEESFQVNI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WVD3 As Substrate

Q8WVD3

Site	PTM Type	Source
A2	Acetylation	Uniprot
K26	Ubiquitination	Uniprot
T27	Phosphorylation	Uniprot
K41	Acetylation	Uniprot
K41	Ubiquitination	Uniprot
R80	Methylation	Uniprot
K94	Ubiquitination	Uniprot
Y108	Phosphorylation	Uniprot
Y112	Phosphorylation	Uniprot
S124	Phosphorylation	Uniprot
S127	Phosphorylation	Uniprot
S137	Phosphorylation	Uniprot
S139	Phosphorylation	Uniprot
T142	Phosphorylation	Uniprot
T144	Phosphorylation	Uniprot
Y145	Phosphorylation	Uniprot
T149	Phosphorylation	Uniprot
K158	Ubiquitination	Uniprot

Research Backgrounds

Q8WVD3_RN138_HUMAN

Function:

E3 ubiquitin-protein ligase involved in DNA damage response by promoting DNA resection and homologous recombination. Recruited to sites of double-strand breaks following DNA damage and specifically promotes double-strand break repair via homologous recombination. Two different, non-exclusive, mechanisms have been proposed. According to a report, regulates the choice of double-strand break repair by favoring homologous recombination over non-homologous end joining (NHEJ): acts by mediating ubiquitination of XRCC5/Ku80, leading to remove the Ku complex from DNA breaks, thereby promoting homologous recombination. According to another report, cooperates with UBE2Ds E2 ubiquitin ligases (UBE2D1, UBE2D2, UBE2D3 or UBE2D4) to promote homologous recombination by mediating ubiquitination of RBBP8/CtIP. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.

PTMs:

Auto-ubiquitinated.

Subcellular Location:

Chromosome.
Note: Recruited at DNA damage sites (PubMed:26502055). Localizes to sites of double-strand break: localization to double-strand break sites is mediated by the zinc fingers (PubMed:26502055, PubMed:26502057).

Subunit Structure:

Interacts with NLK. Interacts with XRCC5/Ku80. Interacts with RBBP8/CtIP.

Family&Domains:

The zinc finger domains (C2H2-type and C2HC-type zinc fingers) bind DNA and mediate recruitment to double-strand break sites. They show strong preference for DNA with 5'- or 3'-single-stranded overhangs, while they do not bind blunt-ended double-stranded DNA or poly(ADP-ribose) (PAR) polymers.

References

1). Downregulation of RNF138 inhibits cellular proliferation, migration, invasion and EMT in glioma cells via suppression of the Erk signaling pathway. Oncology Reports, 2018 (PubMed: 30272353) [IF=4.2]

Application: WB Species: human Sample: human glioma cell lines U87 and U251

Figure 2.| Stable downregulation RNF138 expression in the human glioma cell lines U87 and U251. (A) Western blot assay demonstrated RNF138 protein expression levels in cells transduced with RNF138‑siRNA lentivirus and the negative control lentivirus. GAPDH served as the loading control.

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RNF138 Antibody - #DF4025