DNAI2 Antibody | Affinity Biosciences

1/2

Western blot analysis of extracts from various samples, using DNAI2 Antibody.

DF4013 at 1/100 staining rat testis tissue by IHC-P.

Product:	DNAI2 Antibody
Catalog:	DF4013
Description:	Rabbit polyclonal antibody to DNAI2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	69 KD; 69kD(Calculated).
Uniprot:	Q9GZS0
RRID:	AB_2836373

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(82%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

DNAI2 Antibody detects endogenous levels of total DNAI2.

RRID:

AB_2836373
Cite Format: Affinity Biosciences Cat# DF4013, RRID:AB_2836373.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

axonemal; Axonemal dynein intermediate chain 2; CILD9; DNAI 2; Dnai2; DNAI2_HUMAN; Dynein axonemal intermediate chain 2; Dynein axonemal intermediate polypeptide 2; Dynein intermediate chain 2; Dynein intermediate chain 2 axonemal;

Immunogens

Immunogen:

Uniprot:

Q9GZS0(DNAI2_HUMAN) >>Visit HPA database.

Gene(ID):

DNAI2(64446)

Expression:

Q9GZS0 DNAI2_HUMAN:

Highly expressed in trachea and testis.

Sequence:

Q9GZS0 DNAI2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEIVYVYVKKRSEFGKQCNFSDRQAELNIDIMPNPELAEQFVERNPVDTGIQCSISMSEHEANSERFEMETRGVNHVEGGWPKDVNPLELEQTIRFRKKVEKDENYVNAIMQLGSIMEHCIKQNNAIDIYEEYFNDEEAMEVMEEDPSAKTINVFRDPQEIKRAATHLSWHPDGNRKLAVAYSCLDFQRAPVGMSSDSYIWDLENPNKPELALKPSSPLVTLEFNPKDSHVLLGGCYNGQIACWDTRKGSLVAELSTIESSHRDPVYGTIWLQSKTGTECFSASTDGQVMWWDIRKMSEPTEVVILDITKKEQLENALGAISLEFESTLPTKFMVGTEQGIVISCNRKAKTSAEKIVCTFPGHHGPIYALQRNPFYPKNFLTVGDWTARIWSEDSRESSIMWTKYHMAYLTDAAWSPVRPTVFFTTRMDGTLDIWDFMFEQCDPTLSLKVCDEALFCLRVQDNGCLIACGSQLGTTTLLEVSPGLSTLQRNEKNVASSMFERETRREKILEARHREMRLKEKGKAEGRDEEQTDEELAVDLEALVSKAEEEFFDIIFAELKKKEADAIKLTPVPQQPSPEEDQVVEEGEEAAGEEGDEEVEEDLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZS0 As Substrate

Q9GZS0

Site	PTM Type	Source
S298	Phosphorylation	Uniprot
T301	Phosphorylation	Uniprot
T331	Phosphorylation	Uniprot
T337	Phosphorylation	Uniprot
S344	Phosphorylation	Uniprot
Y405	Phosphorylation	Uniprot
Y409	Phosphorylation	Uniprot
K520	Acetylation	Uniprot
K522	Acetylation	Uniprot

Research Backgrounds

Q9GZS0_DNAI2_HUMAN

Function:

Part of the dynein complex of respiratory cilia.

Subcellular Location:

Cytoplasm>Cytoskeleton>Cilium axoneme.

Tissue Specificity:

Highly expressed in trachea and testis.

Subunit Structure:

Consists of at least two heavy chains and a number of intermediate and light chains (Probable). Interacts with DNAAF2 (By similarity). Interacts with PIH1D3 (By similarity). Interacts with HEATR2; probably involved in outer arm dynein assembly.

Family&Domains:

Belongs to the dynein intermediate chain family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

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DNAI2 Antibody - #DF4013