TESK1 Antibody | Affinity Biosciences

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TESK1 Antibody - Western blot analysis of extracts from rat heart cells using TESK1 antibody.

Product:	TESK1 Antibody
Catalog:	DF4012
Description:	Rabbit polyclonal antibody to TESK1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	68 KD; 68kD(Calculated).
Uniprot:	Q15569
RRID:	AB_2836372

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TESK1 Antibody detects endogenous levels of total TESK1.

RRID:

AB_2836372
Cite Format: Affinity Biosciences Cat# DF4012, RRID:AB_2836372.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Dual specificity testis specific protein kinase 1; Dual specificity testis-specific protein kinase 1; TESK 1; TESK1; TESK1_HUMAN; Testicular protein kinase 1; Testis specific kinase 1; Testis specific protein kinase 1;

Immunogens

Immunogen:

Uniprot:

Q15569(TESK1_HUMAN) >>Visit HPA database.

Gene(ID):

TESK1(7016)

Sequence:

Q15569 TESK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGERPPLRGPGPGPGEVPGEGPPGPGGTGGGPGRGRPSSYRALRSAVSSLARVDDFHCAEKIGAGFFSEVYKVRHRQSGQVMVLKMNKLPSNRGNTLREVQLMNRLRHPNILRFMGVCVHQGQLHALTEYMNGGTLEQLLSSPEPLSWPVRLHLALDIARGLRYLHSKGVFHRDLTSKNCLVRREDRGFTAVVGDFGLAEKIPVYREGARKEPLAVVGSPYWMAPEVLRGELYDEKADVFAFGIVLCELIARVPADPDYLPRTEDFGLDVPAFRTLVGDDCPLPFLLLAIHCCNLEPSTRAPFTEITQHLEWILEQLPEPAPLTRTALTHNQGSVARGGPSATLPRPDPRLSRSRSDLFLPPSPESPPNWGDNLTRVNPFSLREDLRGGKIKLLDTPSKPVLPLVPPSPFPSTQLPLVTTPETLVQPGTPARRCRSLPSSPELPRRMETALPGPGPPAVGPSAEEKMECEGSSPEPEPPGPAPQLPLAVATDNFISTCSSASQPWSPRSGPVLNNNPPAVVVNSPQGWAGEPWNRAQHSLPRAAALERTEPSPPPSAPREPDEGLPCPGCCLGPFSFGFLSMCPRPTPAVARYRNLNCEAGSLLCHRGHHAKPPTPSLQLPGARS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15569 As Substrate

Q15569

Site	PTM Type	Enzyme	Source
R37	Methylation		Uniprot
S69	Phosphorylation		Uniprot
K179	Ubiquitination		Uniprot
K212	Ubiquitination		Uniprot
S220	Phosphorylation	Q15569 (TESK1)	Uniprot
R338	Methylation		Uniprot
S342	Phosphorylation		Uniprot
T344	Phosphorylation		Uniprot
S357	Phosphorylation		Uniprot
K391	Acetylation		Uniprot
K393	Acetylation		Uniprot
K400	Acetylation		Uniprot
S437	Phosphorylation		Uniprot
S441	Phosphorylation		Uniprot
S540	Phosphorylation		Uniprot
S553	Phosphorylation		Uniprot
S603	Phosphorylation		Uniprot
T616	Phosphorylation		Uniprot

PTMs - Q15569 As Enzyme

Q15569

Substrate	Site	Source
P23528 (CFL1)	S3	Uniprot
Q15569 (TESK1)	S220	Uniprot
Q9Y281 (CFL2)	S3	Uniprot

Research Backgrounds

Q15569_TESK1_HUMAN

Function:

Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Probably plays a central role at and after the meiotic phase of spermatogenesis (By similarity).

PTMs:

Autophosphorylated on serine and tyrosine residues.

Subunit Structure:

Interacts with TAOK2; leading to inhibit TAOK2 activity. Interacts with SPRED1; leading to inhibit activity (By similarity). Interacts with SPRY4.

Family&Domains:

The extracatalytic C-terminal part is highly rich in proline residues.

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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TESK1 Antibody - #DF4012