DNAJC5 Antibody - #DF4011

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Product: DNAJC5 Antibody
Catalog: DF4011
Description: Rabbit polyclonal antibody to DNAJC5
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 30 KD; 22kD(Calculated).
Uniprot: Q9H3Z4
RRID: AB_2836371

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
DNAJC5 Antibody detects endogenous levels of total DNAJC5.
RRID:
AB_2836371
Cite Format: Affinity Biosciences Cat# DF4011, RRID:AB_2836371.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CLN 4; CLN4; CLN4B; CSP; cysteine string protein alpha; Cysteine string protein; DJC5; DnaJ (Hsp40) homolog subfamily C member 5; DnaJ homolog subfamily C member 5; DNAJC 5; Dnajc5; DNAJC5A; DNJC5_HUMAN; NCL;

Immunogens

Immunogen:

A synthesized peptide derived from human DNAJC5, corresponding to a region within the internal amino acids.

Uniprot:

Q9H3Z4(DNJC5_HUMAN) >>Visit HPA database.

Gene(ID):
DNAJC5(80331)
Expression:
Q9H3Z4 DNJC5_HUMAN:

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Sequence:
MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVFCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Bovine
91
Chicken
90
Rabbit
90
Xenopus
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Acts as a general chaperone in regulated exocytosis (By similarity). Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity). Involved in the calcium-mediated control of a late stage of exocytosis (By similarity). May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).

PTMs:

Fatty acylated (By similarity). Heavily palmitoylated in the cysteine string motif (By similarity).

Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58. This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions.

Subcellular Location:

Membrane>Lipid-anchor. Melanosome. Cell membrane.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

References

1). DNAJC5 promotes cisplatin resistance in epithelial ovarian cancer by autophagy induced by the BiP/IRE1α/XBP1 endoplasmic reticulum stress pathway. Scientific reports, 2025 (PubMed: 41038875) [IF=3.8]

Application: WB    Species: human    Sample: EOC cell

Fig. 2 Overexpression of DNAJC5 promotes DDP resistance in EOC cells in vitro. (a) Expression levels of DNAJC5 in different EOC cell lines. (b) Western blot analysis confirming DNAJC5 protein expression in A2780 cells infected with a lentivirus overexpressing DNAJC5. (c) A2780 and A2780/DNAJC5 cells were incubated with varying concentrations of DDP (50µM, 40µM, 30µM, 20µM, 10µM, 5 µM, 2.5µM, 1.25µM) for 72 h. Cells were then treated with CCK-8 reagent in the dark for 1.5 h, and the final IC50 values were determined. (d,e) Clonogenic assay of A2780/DNAJC5 cells. A total of 300 cells per well were seeded and treated with 3µM DDP after 24 h. After 10 days, colonies were stained with crystal violet and quantified using ImageJ software. The bar graph represents the effect of DDP on the viability of A2780 and A2780/DNAJC5 cells. (f,g) Apoptosis assay of A2780/DNAJC5 cells following treatment with 3µM DDP for 72 h. The bar graph illustrates the average apoptosis rate of A2780/DNAJC5 cells under DDP treatment compared to the Vector control.

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