Product: DNAJC5 Antibody
Catalog: DF4011
Description: Rabbit polyclonal antibody to DNAJC5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 30 KD; 22kD(Calculated).
Uniprot: Q9H3Z4
RRID: AB_2836371

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
DNAJC5 Antibody detects endogenous levels of total DNAJC5.
RRID:
AB_2836371
Cite Format: Affinity Biosciences Cat# DF4011, RRID:AB_2836371.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CLN 4; CLN4; CLN4B; CSP; cysteine string protein alpha; Cysteine string protein; DJC5; DnaJ (Hsp40) homolog subfamily C member 5; DnaJ homolog subfamily C member 5; DNAJC 5; Dnajc5; DNAJC5A; DNJC5_HUMAN; NCL;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9H3Z4 DNJC5_HUMAN:

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Sequence:
MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEINNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVFCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Bovine
91
Chicken
90
Rabbit
90
Xenopus
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H3Z4 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
S10 Phosphorylation P31749 (AKT1) , P17612 (PRKACA)
T11 Phosphorylation
S12 Phosphorylation
S15 Phosphorylation
Y17 Phosphorylation
K24 Ubiquitination
S34 Phosphorylation
K41 Acetylation
K41 Ubiquitination
Y42 Phosphorylation
K46 Ubiquitination
K56 Acetylation
K56 Ubiquitination
K58 Ubiquitination
K72 Ubiquitination
Y149 Phosphorylation
Y192 Phosphorylation

Research Backgrounds

Function:

Acts as a general chaperone in regulated exocytosis (By similarity). Acts as a co-chaperone for the SNARE protein SNAP-25 (By similarity). Involved in the calcium-mediated control of a late stage of exocytosis (By similarity). May have an important role in presynaptic function. May be involved in calcium-dependent neurotransmitter release at nerve endings (By similarity).

PTMs:

Fatty acylated (By similarity). Heavily palmitoylated in the cysteine string motif (By similarity).

Ser-10 phosphorylation induces an order-to-disorder transition triggering the interaction with Lys-58. This conformational switch modulates DNAJC5's cellular functions by reducing binding to syntaxin and synaptogamin without altering HSC70 interactions.

Subcellular Location:

Membrane>Lipid-anchor. Melanosome. Cell membrane.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.

Subunit Structure:

Oligomers. Homodimer (By similarity). Interacts with the chaperone complex consisting of HSC70 and SGTA (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats). Interacts with SYT1, SYT5 and SYT7, and with SYT9, forming a complex with SNAP25 (By similarity).

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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