POLR1C Antibody - #DF4008

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Product: POLR1C Antibody
Catalog: DF4008
Description: Rabbit polyclonal antibody to POLR1C
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 39 KD; 39kD(Calculated).
Uniprot: O15160
RRID: AB_2836368

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
POLR1C Antibody detects endogenous levels of total POLR1C.
RRID:
AB_2836368
Cite Format: Affinity Biosciences Cat# DF4008, RRID:AB_2836368.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40kDa; AA409007; AA959927; Ac2-127; AC40; AL024089; DNA directed RNA polymerase I subunit C; DNA directed RNA polymerases I and III 40 kDa polypeptide; DNA directed RNA polymerases I and III subunit RPAC1; DNA-directed RNA polymerase I subunit C; DNA-directed RNA polymerases I and III 40 kDa polypeptide; DNA-directed RNA polymerases I and III subunit RPAC1; MGC105583; MGC161175; POLR1C; POLR1E; Polymerase (RNA) I polypeptide C, 30kDa; RNA polymerase 1 1; RNA polymerases I and III subunit AC1; RP3-337H4.4; RPA39; RPA40; RPA5; RPAC1; RPAC1_HUMAN; RPC40; Rpo1 1; TCS3;

Immunogens

Immunogen:
Uniprot:

O15160(RPAC1_HUMAN) >>Visit HPA database.

Gene(ID):
POLR1C(9533)
Sequence:
MAASQAVEEMRSRVVLGEFGVRNVHTTDFPGNYSGYDDAWDQDRFEKNFRVDVVHMDENSLEFDMVGIDAAIANAFRRILLAEVPTMAVEKVLVYNNTSIVQDEILAHRLGLIPIHADPRLFEYRNQGDEEGTEIDTLQFRLQVRCTRNPHAAKDSSDPNELYVNHKVYTRHMTWIPLGNQADLFPEGTIRPVHDDILIAQLRPGQEIDLLMHCVKGIGKDHAKFSPVATASYRLLPDITLLEPVEGEAAEELSRCFSPGVIEVQEVQGKKVARVANPRLDTFSREIFRNEKLKKVVRLARVRDHYIFSVESTGVLPPDVLVSEAIKVLMGKCRRFLDELDAVQMD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Zebrafish
73
Xenopus
64
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15160 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation
Y33 Phosphorylation
S34 Phosphorylation
Y36 Phosphorylation
K47 Ubiquitination
K91 Ubiquitination
R120 Methylation
Y124 Phosphorylation
R125 Methylation
K154 Ubiquitination
S157 Phosphorylation
K167 Ubiquitination
K220 Ubiquitination
K224 Ubiquitination
S226 Phosphorylation
C256 S-Nitrosylation
S258 Phosphorylation
K270 Ubiquitination
T282 Phosphorylation
S284 Phosphorylation
K332 Ubiquitination

Research Backgrounds

Function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the RNA polymerase I (Pol I) and RNA polymerase III (Pol III) complexes consisting of at least 13 and 17 subunits, respectively.

Family&Domains:

Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.

Research Fields

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

Restrictive clause

 

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