RPC3 Antibody - #DF4005
| Product: | RPC3 Antibody |
| Catalog: | DF4005 |
| Description: | Rabbit polyclonal antibody to RPC3 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 60 KD; 61kD(Calculated). |
| Uniprot: | Q9BUI4 |
| RRID: | AB_2836365 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4005, RRID:AB_2836365.
Fold/Unfold
DNA directed III 62 kDa polypeptide; DNA-directed RNA polymerase III subunit C; DNA-directed RNA polymerase III subunit rpc3; POLR3C; Polymerase (RNA) III (DNA directed) polypeptide C (62kD); RNA polymerase C subunit 3; RNA polymerase III 62 kDa subunit; RNA polymerase III C62 subunit; RNA polymerase III subunit C3; RPC3; RPC3_HUMAN; RPC62;
Immunogens
- Q9BUI4 RPC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTQAEIKLCSLLLQEHFGEIVEKIGVHLIRTGSQPLRVIAHDTGTSLDQVKKALCVLVQHNLVSYQVHKRGVVEYEAQCSRVLRMLRYPRYIYTTKTLYSDTGELIVEELLLNGKLTMSAVVKKVADRLTETMEDGKTMDYAEVSNTFVRLADTHFVQRCPSVPTTENSDPGPPPPAPTLVINEKDMYLVPKLSLIGKGKRRRSSDEDAAGEPKAKRPKYTTDNKEPIPDDGIYWQANLDRFHQHFRDQAIVSAVANRMDQTSSEIVRTMLRMSEITTSSSAPFTQPLSSNEIFRSLPVGYNISKQVLDQYLTLLADDPLEFVGKSGDSGGGMYVINLHKALASLATATLESVVQERFGSRCARIFRLVLQKKHIEQKQVEDFAMIPAKEAKDMLYKMLSENFMSLQEIPKTPDHAPSRTFYLYTVNILSAARMLLHRCYKSIANLIERRQFETKENKRLLEKSQRVEAIIASMQATGAEEAQLQEIEEMITAPERQQLETLKRNVNKLDASEIQVDETIFLLESYIECTMKRQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BUI4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S33 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| C79 | S-Nitrosylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| Y334 | Phosphorylation | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K389 | Acetylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| T501 | Phosphorylation | Uniprot | |
| K503 | Ubiquitination | Uniprot |
Research Backgrounds
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner.
Nucleus.
Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex. Directly interacts with POLR3G and POLR3GL. Directly interacts with POLR3F/RPC39. Interacts with GTF3C4.
Belongs to the eukaryotic RPC3/POLR3C RNA polymerase subunit family.
Research Fields
· Genetic Information Processing > Transcription > RNA polymerase.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)
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