RPC1 Antibody - #DF4004

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Product: RPC1 Antibody
Catalog: DF4004
Description: Rabbit polyclonal antibody to RPC1
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 156 KD; 156kD(Calculated).
Uniprot: O14802
RRID: AB_2836364

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RPC1 Antibody detects endogenous levels of total RPC1.
RRID:
AB_2836364
Cite Format: Affinity Biosciences Cat# DF4004, RRID:AB_2836364.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BC053071; DNA directed RNA polymerase III largest subunit; DNA directed RNA polymerase III subunit A; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; DNA-directed RNA polymerase III subunit RPC1; hRPC155; MGC62420; POLR 3A; POLR3A; Polymerase (RNA) III (DNA directed) polypeptide A 155kDa; Polymerase (RNA) III (DNA directed) polypeptide A; RGD1305574; RNA polymerase III 155 kDa subunit; RNA polymerase III subunit C1; RNA polymerase III subunit C160; RNA polymerase III subunit RPC155 D; RPC1; RPC1_HUMAN; RPC155;

Immunogens

Immunogen:
Uniprot:

O14802(RPC1_HUMAN) >>Visit HPA database.

Gene(ID):
POLR3A(11128)
Expression:
O14802 RPC1_HUMAN:

Expressed in the brain, in the cortex and the white matter (at protein level).

Sequence:
MVKEQFRETDVAKKISHICFGMKSPEEMRQQAHIQVVSKNLYSQDNQHAPLLYGVLDHRMGTSEKDRPCETCGKNLADCLGHYGYIDLELPCFHVGYFRAVIGILQMICKTCCHIMLSQEEKKQFLDYLKRPGLTYLQKRGLKKKISDKCRKKNICHHCGAFNGTVKKCGLLKIIHEKYKTNKKVVDPIVSNFLQSFETAIEHNKEVEPLLGRAQENLNPLVVLNLFKRIPAEDVPLLLMNPEAGKPSDLILTRLLVPPLCIRPSVVSDLKSGTNEDDLTMKLTEIIFLNDVIKKHRISGAKTQMIMEDWDFLQLQCALYINSELSGIPLNMAPKKWTRGFVQRLKGKQGRFRGNLSGKRVDFSGRTVISPDPNLRIDEVAVPVHVAKILTFPEKVNKANINFLRKLVQNGPEVHPGANFIQQRHTQMKRFLKYGNREKMAQELKYGDIVERHLIDGDVVLFNRQPSLHKLSIMAHLARVKPHRTFRFNECVCTPYNADFDGDEMNLHLPQTEEAKAEALVLMGTKANLVTPRNGEPLIAAIQDFLTGAYLLTLKDTFFDRAKACQIIASILVGKDEKIKVRLPPPTILKPVTLWTGKQIFSVILRPSDDNPVRANLRTKGKQYCGKGEDLCANDSYVTIQNSELMSGSMDKGTLGSGSKNNIFYILLRDWGQNEAADAMSRLARLAPVYLSNRGFSIGIGDVTPGQGLLKAKYELLNAGYKKCDEYIEALNTGKLQQQPGCTAEETLEALILKELSVIRDHAGSACLRELDKSNSPLTMALCGSKGSFINISQMIACVGQQAISGSRVPDGFENRSLPHFEKHSKLPAAKGFVANSFYSGLTPTEFFFHTMAGREGLVDTAVKTAETGYMQRRLVKSLEDLCSQYDLTVRSSTGDIIQFIYGGDGLDPAAMEGKDEPLEFKRVLDNIKAVFPCPSEPALSKNELILTTESIMKKSEFLCCQDSFLQEIKKFIKGVSEKIKKTRDKYGINDNGTTEPRVLYQLDRITPTQVEKFLETCRDKYMRAQMEPGSAVGALCAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTLLGEISEYIEEVFLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDVAVHGEAVVCVTPRENSKSSMYYVLQFLKEDLPKVVVQGIPEVSRAVIHIDEQSGKEKYKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRPLIFDTNEFHIPLVT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14802 As Substrate

Site PTM Type Enzyme
K3 Methylation
K23 Acetylation
S24 Phosphorylation
Y53 Phosphorylation
K65 Acetylation
K65 Ubiquitination
K139 Ubiquitination
K178 Ubiquitination
K184 Ubiquitination
K228 Ubiquitination
K246 Ubiquitination
K271 Ubiquitination
K346 Acetylation
K348 Acetylation
R353 Methylation
K359 Acetylation
K388 Ubiquitination
K395 Ubiquitination
K398 Ubiquitination
K406 Ubiquitination
K433 Ubiquitination
Y434 Phosphorylation
K445 Acetylation
K445 Ubiquitination
T525 Phosphorylation
K526 Ubiquitination
K590 Ubiquitination
T593 Phosphorylation
T596 Phosphorylation
K652 Ubiquitination
K660 Ubiquitination
S681 Phosphorylation
K711 Ubiquitination
K713 Ubiquitination
Y714 Phosphorylation
K722 Ubiquitination
K723 Ubiquitination
K735 Ubiquitination
K754 Ubiquitination
K773 Ubiquitination
K823 Ubiquitination
K864 Ubiquitination
K877 Ubiquitination
K922 Ubiquitination
S936 Phosphorylation
S941 Phosphorylation
T948 Phosphorylation
T949 Phosphorylation
K955 Ubiquitination
K970 Ubiquitination
K974 Ubiquitination
S977 Phosphorylation
K986 Ubiquitination
R1005 Methylation
K1013 Ubiquitination
T1046 Phosphorylation
T1049 Phosphorylation
K1071 Ubiquitination
K1078 Ubiquitination
K1091 Ubiquitination
K1102 Ubiquitination
K1157 Ubiquitination
S1181 Phosphorylation
K1182 Ubiquitination
K1198 Ubiquitination
K1224 Ubiquitination
K1242 Ubiquitination
T1247 Phosphorylation
T1251 Phosphorylation
K1256 Ubiquitination
T1304 Phosphorylation
K1365 Acetylation
K1365 Ubiquitination
K1374 Ubiquitination

Research Backgrounds

Function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the brain, in the cortex and the white matter (at protein level).

Subunit Structure:

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.

Family&Domains:

Belongs to the RNA polymerase beta' chain family.

Research Fields

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

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