CD3EAP Antibody - #DF4002

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Product: CD3EAP Antibody
Catalog: DF4002
Description: Rabbit polyclonal antibody to CD3EAP
Application: WB IF/ICC
Reactivity: Human
Mol.Wt.: 55 KD; 55kD(Calculated).
Uniprot: O15446
RRID: AB_2836362

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
CD3EAP Antibody detects endogenous levels of total CD3EAP.
RRID:
AB_2836362
Cite Format: Affinity Biosciences Cat# DF4002, RRID:AB_2836362.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A34.5; Anti sense to ERCC 1 protein; Antisense to ERCC-1 protein; ASE 1; ASE-1; ASE1; CAST; CD3 epsilon associated protein; CD3-epsilon-associated protein; CD3E antigen, epsilon polypeptide associated protein; CD3E associated protein; CD3e molecule, epsilon associated protein; CD3E-associated protein; CD3EAP; DNA directed RNA polymerase I subunit RPA34; DNA-directed RNA polymerase I subunit RPA34; MGC118851; PAF 49; RNA polymerase I associated factor PAF49; RNA polymerase I-associated factor PAF49; RPA34_HUMAN;

Immunogens

Immunogen:
Uniprot:

O15446(RPA34_HUMAN) >>Visit HPA database.

Gene(ID):
CD3EAP(10849)
Sequence:
MEEPQAGDAARFSCPPNFTAKPPASESPRFSLEALTGPDTELWLIQAPADFAPECFNGRHVPLSGSQIVKGKLAGKRHRYRVLSSCPQAGEATLLAPSTEAGGGLTCASAPQGTLRILEGPQQSLSGSPLQPIPASPPPQIPPGLRPRFCAFGGNPPVTGPRSALAPNLLTSGKKKKEMQVTEAPVTQEAVNGHGALEVDMALGSPEMDVRKKKKKKNQQLKEPEAAGPVGTEPTVETLEPLGVLFPSTTKKRKKPKGKETFEPEDKTVKQEQINTEPLEDTVLSPTKKRKRQKGTEGMEPEEGVTVESQPQVKVEPLEEAIPLPPTKKRKKEKGQMAMMEPGTEAMEPVEPEMKPLESPGGTMAPQQPEGAKPQAQAALAAPKKKTKKEKQQDATVEPETEVVGPELPDDLEPQAAPTSTKKKKKKKERGHTVTEPIQPLEPELPGEGQPEARATPGSTKKRKKQSQESRMPETVPQEEMPGPPLNSESGEEAPTGRDKKRKQQQQQPV

PTMs - O15446 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S27 Phosphorylation
S66 Phosphorylation
K70 Ubiquitination
Y80 Phosphorylation
S124 Phosphorylation
S126 Phosphorylation
S128 Phosphorylation
S136 Phosphorylation
C150 S-Nitrosylation
T159 Phosphorylation
R162 Methylation
S163 Phosphorylation
T171 Phosphorylation
S172 Phosphorylation
K174 Acetylation
S205 Phosphorylation
S248 Phosphorylation
T249 Phosphorylation
K270 Sumoylation
T276 Phosphorylation
T282 Phosphorylation
S285 Phosphorylation P06493 (CDK1)
T287 Phosphorylation
S309 Phosphorylation
K314 Sumoylation
S359 Phosphorylation
T363 Phosphorylation
T419 Phosphorylation
T421 Phosphorylation
T456 Phosphorylation
S459 Phosphorylation
T475 Phosphorylation
S488 Phosphorylation
S490 Phosphorylation

Research Backgrounds

Function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.

Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.

PTMs:

Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other groups.

Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.

Subcellular Location:

Nucleus>Nucleolus. Chromosome.
Note: Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.

Family&Domains:

Belongs to the eukaryotic RPA34 RNA polymerase subunit family.

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