DHODH Antibody - #DF3991

(3)  
Product: DHODH Antibody
Catalog: DF3991
Description: Rabbit polyclonal antibody to DHODH
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 48 KD; 43kD(Calculated).
Uniprot: Q02127
RRID: AB_2836351

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Rabbit(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
DHODH Antibody detects endogenous levels of total DHODH.
RRID:
AB_2836351
Cite Format: Affinity Biosciences Cat# DF3991, RRID:AB_2836351.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHOdehase; Dhodh; Dihydroorotate dehydrogenase (quinone); Dihydroorotate dehydrogenase; Dihydroorotate dehydrogenase mitochondrial; Dihydroorotate oxidase; Human complement of yeast URA1; mitochondrial; POADS; PYRD_HUMAN; URA1;

Immunogens

Immunogen:
Uniprot:

Q02127(PYRD_HUMAN) >>Visit HPA database.

Gene(ID):
DHODH(1723)
Sequence:
MAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
80
Horse
80
Bovine
80
Sheep
80
Dog
80
Rabbit
80
Chicken
78
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02127 As Substrate

Site PTM Type Enzyme
T62 Phosphorylation
S63 Phosphorylation
K86 Ubiquitination
K99 Ubiquitination
T121 Phosphorylation
Y146 Phosphorylation
K169 Ubiquitination
K183 Ubiquitination
K185 Ubiquitination
Y194 Phosphorylation
S214 Phosphorylation
T217 Phosphorylation
K226 Ubiquitination
K235 Ubiquitination
K254 Ubiquitination
K264 Ubiquitination
K306 Ubiquitination
K344 Ubiquitination
Y355 Phosphorylation
T356 Phosphorylation
T359 Phosphorylation
K378 Ubiquitination

Research Backgrounds

Function:

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

PTMs:

The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer.

Family&Domains:

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Research Fields

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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