DHODH Antibody | Affinity Biosciences

WB
IF/ICC

1/3

Western blot analysis of extracts from Jurkat cells, using DHODH antibody.

Western blot analysis of extracts from Rat brain, using DHODH Antibody.

Product:	DHODH Antibody
Catalog:	DF3991
Description:	Rabbit polyclonal antibody to DHODH
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	48 KD; 43kD(Calculated).
Uniprot:	Q02127
RRID:	AB_2836351

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(80%), Bovine(80%), Horse(80%), Sheep(80%), Rabbit(80%), Dog(80%)

Clonality:

Polyclonal

Specificity:

DHODH Antibody detects endogenous levels of total DHODH.

RRID:

AB_2836351
Cite Format: Affinity Biosciences Cat# DF3991, RRID:AB_2836351.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DHOdehase; Dhodh; Dihydroorotate dehydrogenase (quinone); Dihydroorotate dehydrogenase; Dihydroorotate dehydrogenase mitochondrial; Dihydroorotate oxidase; Human complement of yeast URA1; mitochondrial; POADS; PYRD_HUMAN; URA1;

Immunogens

Immunogen:

Uniprot:

Q02127(PYRD_HUMAN) >>Visit HPA database.

Gene(ID):

DHODH(1723)

Sequence:

Q02127 PYRD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAWRHLKKRAQDAVIILGGGGLLFASYLMATGDERFYAEHLMPTLQGLLDPESAHRLAVRFTSLGLLPRARFQDSDMLEVRVLGHKFRNPVGIAAGFDKHGEAVDGLYKMGFGFVEIGSVTPKPQEGNPRPRVFRLPEDQAVINRYGFNSHGLSVVEHRLRARQQKQAKLTEDGLPLGVNLGKNKTSVDAAEDYAEGVRVLGPLADYLVVNVSSPNTAGLRSLQGKAELRRLLTKVLQERDGLRRVHRPAVLVKIAPDLTSQDKEDIASVVKELGIDGLIVTNTTVSRPAGLQGALRSETGGLSGKPLRDLSTQTIREMYALTQGRVPIIGVGGVSSGQDALEKIRAGASLVQLYTALTFWGPPVVGKVKRELEALLKEQGFGGVTDAIGADHRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02127 As Substrate

Q02127

Site	PTM Type	Source
T62	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
K86	Ubiquitination	Uniprot
K99	Ubiquitination	Uniprot
T121	Phosphorylation	Uniprot
Y146	Phosphorylation	Uniprot
K169	Ubiquitination	Uniprot
K183	Ubiquitination	Uniprot
K185	Ubiquitination	Uniprot
Y194	Phosphorylation	Uniprot
S214	Phosphorylation	Uniprot
T217	Phosphorylation	Uniprot
K226	Ubiquitination	Uniprot
K235	Ubiquitination	Uniprot
K254	Ubiquitination	Uniprot
K264	Ubiquitination	Uniprot
K306	Ubiquitination	Uniprot
K344	Ubiquitination	Uniprot
Y355	Phosphorylation	Uniprot
T356	Phosphorylation	Uniprot
T359	Phosphorylation	Uniprot
K378	Ubiquitination	Uniprot

Research Backgrounds

Q02127_PYRD_HUMAN

Function:

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.

PTMs:

The uncleaved transit peptide is required for mitochondrial targeting and proper membrane integration.

Subcellular Location:

Mitochondrion inner membrane>Single-pass membrane protein.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Research Fields

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). GSK-3β inhibition alleviates arthritis pain via reducing spinal mitochondrial reactive oxygen species level and inflammation. PloS one, 2023 (PubMed: 37058473) [IF=3.7]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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DHODH Antibody - #DF3991