DOCK1 Antibody - #DF3984
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3984, RRID:AB_2836344.
Fold/Unfold
180 kDa protein downstream of CRK; Ced5; Dedicator of Cytokinesis 1; Dedicator of cytokinesis protein 1; DOCK 1; DOCK 180; DOCK1; DOCK1_HUMAN; DOCK180;
Immunogens
Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
- Q14185 DOCK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTRWVPTKREEKYGVAFYNYDARGADELSLQIGDTVHILETYEGWYRGYTLRKKSKKGIFPASYIHLKEAIVEGKGQHETVIPGDLPLIQEVTTTLREWSTIWRQLYVQDNREMFRSVRHMIYDLIEWRSQILSGTLPQDELKELKKKVTAKIDYGNRILDLDLVVRDEDGNILDPELTSTISLFRAHEIASKQVEERLQEEKSQKQNIDINRQAKFAATPSLALFVNLKNVVCKIGEDAEVLMSLYDPVESKFISENYLVRWSSSGLPKDIDRLHNLRAVFTDLGSKDLKREKISFVCQIVRVGRMELRDNNTRKLTSGLRRPFGVAVMDVTDIINGKVDDEDKQHFIPFQPVAGENDFLQTVINKVIAAKEVNHKGQGLWVTLKLLPGDIHQIRKEFPHLVDRTTAVARKTGFPEIIMPGDVRNDIYVTLVQGDFDKGSKTTAKNVEVTVSVYDEDGKRLEHVIFPGAGDEAISEYKSVIYYQVKQPRWFETVKVAIPIEDVNRSHLRFTFRHRSSQDSKDKSEKIFALAFVKLMRYDGTTLRDGEHDLIVYKAEAKKLEDAATYLSLPSTKAELEEKGHSATGKSMQSLGSCTISKDSFQISTLVCSTKLTQNVDLLGLLKWRSNTSLLQQNLRQLMKVDGGEVVKFLQDTLDALFNIMMENSESETFDTLVFDALVFIIGLIADRKFQHFNPVLETYIKKHFSATLAYTKLTKVLKNYVDGAEKPGVNEQLYKAMKALESIFKFIVRSRILFNQLYENKGEADFVESLLQLFRSINDMMSSMSDQTVRVKGAALKYLPTIVNDVKLVFDPKELSKMFTEFILNVPMGLLTIQKLYCLIEIVHSDLFTQHDCREILLPMMTDQLKYHLERQEDLEACCQLLSHILEVLYRKDVGPTQRHVQIIMEKLLRTVNRTVISMGRDSELIGNFVACMTAILRQMEDYHYAHLIKTFGKMRTDVVDFLMETFIMFKNLIGKNVYPFDWVIMNMVQNKVFLRAINQYADMLNKKFLDQANFELQLWNNYFHLAVAFLTQESLQLENFSSAKRAKILNKYGDMRRQIGFEIRDMWYNLGQHKIKFIPEMVGPILEMTLIPETELRKATIPIFFDMMQCEFHSTRSFQMFENEIITKLDHEVEGGRGDEQYKVLFDKILLEHCRKHKYLAKTGETFVKLVVRLMERLLDYRTIMHDENKENRMSCTVNVLNFYKEIEREEMYIRYLYKLCDLHKECDNYTEAAYTLLLHAKLLKWSEDVCVAHLTQRDGYQATTQGQLKEQLYQEIIHYFDKGKMWEEAIALGKELAEQYENEMFDYEQLSELLKKQAQFYENIVKVIRPKPDYFAVGYYGQGFPTFLRGKVFIYRGKEYERREDFEARLLTQFPNAEKMKTTSPPGDDIKNSPGQYIQCFTVKPKLDLPPKFHRPVSEQIVSFYRVNEVQRFEYSRPIRKGEKNPDNEFANMWIERTIYTTAYKLPGILRWFEVKSVFMVEISPLENAIETMQLTNDKINSMVQQHLDDPSLPINPLSMLLNGIVDPAVMGGFANYEKAFFTDRYLQEHPEAHEKIEKLKDLIAWQIPFLAEGIRIHGDKVTEALRPFHERMEACFKQLKEKVEKEYGVRIMPSSLDDRRGSRPRSMVRSFTMPSSSRPLSVASVSSLSSDSTPSRPGSDGFALEPLLPKKMHSRSQDKLDKDDLEKEKKDKKKEKRNSKHQEIFEKEFKPTDISLQQSEAVILSETISPLRPQRPKSQVMNVIGSERRFSVSPSSPSSQQTPPPVTPRAKLSFSMQSSLELNGMTGADVADVPPPLPLKGSVADYGNLMENQDLLGSPTPPPPPPHQRHLPPPLPSKTPPPPPPKTTRKQASVDSGIVQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14185 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S100 | Phosphorylation | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| Y107 | Phosphorylation | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| T314 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| R405 | Methylation | Uniprot | |
| Y478 | Phosphorylation | Uniprot | |
| Y483 | Phosphorylation | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| T566 | Phosphorylation | Uniprot | |
| Y567 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| K574 | Ubiquitination | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| K587 | Ubiquitination | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S591 | Phosphorylation | Uniprot | |
| Y722 | Phosphorylation | Uniprot | |
| K728 | Ubiquitination | Uniprot | |
| Y760 | Phosphorylation | Uniprot | |
| S784 | Phosphorylation | Uniprot | |
| K799 | Ubiquitination | Uniprot | |
| Y800 | Phosphorylation | Uniprot | |
| T803 | Phosphorylation | Uniprot | |
| K815 | Ubiquitination | Uniprot | |
| S818 | Phosphorylation | Uniprot | |
| K1054 | Acetylation | Uniprot | |
| K1054 | Ubiquitination | Uniprot | |
| K1146 | Ubiquitination | Uniprot | |
| K1165 | Ubiquitination | Uniprot | |
| S1250 | Phosphorylation | Uniprot | |
| K1286 | Ubiquitination | Uniprot | |
| Y1304 | Phosphorylation | Uniprot | |
| K1320 | Ubiquitination | Uniprot | |
| K1330 | Ubiquitination | Uniprot | |
| K1560 | Ubiquitination | Uniprot | |
| K1602 | Acetylation | Uniprot | |
| K1605 | Acetylation | Uniprot | |
| K1610 | Ubiquitination | Uniprot | |
| Y1612 | Phosphorylation | Uniprot | |
| T1637 | Phosphorylation | Uniprot | |
| S1642 | Phosphorylation | Uniprot | |
| S1664 | Phosphorylation | Uniprot | |
| S1681 | Phosphorylation | Uniprot | |
| S1704 | Phosphorylation | Uniprot | |
| S1743 | Phosphorylation | Uniprot | |
| S1751 | Phosphorylation | Uniprot | |
| S1756 | Phosphorylation | Uniprot | |
| S1758 | Phosphorylation | Uniprot | |
| S1763 | Phosphorylation | Uniprot | |
| T1767 | Phosphorylation | Uniprot | |
| T1772 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S1807 | Phosphorylation | Uniprot | |
| Y1811 | Phosphorylation | Uniprot | |
| S1823 | Phosphorylation | Uniprot | |
| T1825 | Phosphorylation | Uniprot | |
| S1842 | Phosphorylation | Uniprot | |
| T1844 | Phosphorylation | Uniprot | |
| S1858 | Phosphorylation | Uniprot |
Research Backgrounds
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.
Cytoplasm. Membrane.
Note: Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate.
Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.
Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with ADGRB1. Identified in a complex with AUTS2 and ELMO2 (By similarity).
The DHR-2 domain is necessary and sufficient for the GEF activity.
Belongs to the DOCK family.
Research Fields
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
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