AGBL4 Antibody | Affinity Biosciences

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AGBL4 Antibody - Figure 3 The flowchart of the whole analysis process and AGBL4 gene validation.

Product:	AGBL4 Antibody
Catalog:	DF3981
Description:	Rabbit polyclonal antibody to AGBL4
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	62 KD; 58kD(Calculated).
Uniprot:	Q5VU57
RRID:	AB_2836341

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

AGBL4 Antibody detects endogenous levels of total AGBL4.

RRID:

AB_2836341
Cite Format: Affinity Biosciences Cat# DF3981, RRID:AB_2836341.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AGBL4; ATP/GTP binding protein like 4; ATP/GTP-binding protein-like 4; CBPC6_HUMAN; CCP6; Cytosolic carboxypeptidase 6; FLJ14442;

Immunogens

Immunogen:

Uniprot:

Q5VU57(CBPC6_HUMAN) >>Visit HPA database.

Gene(ID):

AGBL4(84871)

Sequence:

Q5VU57 CBPC6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEGSQSAPEAGNDMGNDDAIGGNVSKYIVLPTGYCGQPKKGHLIFDACFESGNLGRVDQVSEFEYDLFIRPDTCNPRFRVWFNFTVENVKESQRVIFNIVNFSKTKSLYRDGMAPMVKSTSRPKWQRLPPKNVYYYRCPDHRKNYVMSFAFCFDREEDIYQFAYCYPYTYTRFQHYLDSLQKRNMDYFFREQLGQSVQQRKLDLLTITSPDNLREGAEQKVVFITGRVHPGETPSSFVCQGIIDFLVSQHPIACVLREYLVFKIAPMLNPDGVYLGNYRCSLMGFDLNRHWLDPSPWVHPTLHGVKQLIVQMYNDPKTSLEFYIDIHAHSTMMNGFMYGNIFEDEERFQRQAIFPKLLCQNAEDFSYSSTSFNRDAVKAGTGRRFLGGLLDHTSYCYTLEVSFYSYIISGTTAAVPYTEEAYMKLGRNVARTFLDYYRLNPVVEKVAIPMPRLRNKEIEVQRRKEKSPPYKHPLLRGPASNYPNSKGDKKSSVNHKDPSTPF

PTMs - Q5VU57 As Substrate

Q5VU57

Site	PTM Type	Enzyme	Source
Y275	Phosphorylation		Uniprot
T382	Phosphorylation		Uniprot

Research Backgrounds

Q5VU57_CBPC6_HUMAN

Function:

Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes polyglutamates from the carboxy-terminus of target proteins such as MYLK. Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5.

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Golgi apparatus. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Colocalizes with gamma-tubulin in the centrioles at interphase and dividing cells and with glutamylated tubulin in basal bodies of ciliated cells.

Subunit Structure:

Interacts with MYLK.

Family&Domains:

Belongs to the peptidase M14 family.

References

1). One potential biomarker for teratozoospermia identified by in-depth integrative analysis of multiple microarray data. Aging (Albany NY), 2021 (PubMed: 33819193) [IF=3.9]

Application: WB Species: Human Sample:

Figure 3 The flowchart of the whole analysis process and AGBL4 gene validation. (A) The flowchart of the whole analysis process. After collecting the datasets GSE6872 and GSE6967, we combined differentially expressed gene screening, GSEA analysis, and WGCNA analysis to narrow down the most relevant modules (separately colored with turquoise and yellow) between the two datasets. Subsequently, after two intersections, three DEGs were screened. Then, we substituted these three differentially expressed genes into the other dataset (GSE6968) for validation and found that only AGBL4 gene had an identical expression trend to the first two datasets (GSE6872 and GSE6967). (B) AGBL4 gene validation in another dataset GSE6968. TZ, teratozoospermia samples; control, healthy samples. (C) AGBL4 gene expression validation using western blotting. TZ, teratozoospermia samples; control, healthy samples. The fold change calculation was finished based on gray intensities of protein bands. (D) AGBL4 gene expression validation using qRT-PCR. TZ, teratozoospermia samples; control, healthy samples.

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AGBL4 Antibody - #DF3981