ATG4C Antibody - #DF3976
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF3976, RRID:AB_2836336.
Fold/Unfold
APG4 autophagy 4 homolog C (S. cerevisiae); APG4 autophagy 4 homolog C; APG4 C; APG4-C; APG4C; ATG 4C; ATG4 autophagy related 4 homolog C (S. cerevisiae); ATG4 autophagy related 4 homolog C; Atg4c; ATG4C_HUMAN; AUT (S. cerevisiae) like 1, cysteine endopeptidase; AUT like 1, cysteine endopeptidase (S. cerevisiae); AUT like 1, cysteine endopeptidase; AUT like 3 cysteine endopeptidase; AUT-like 3 cysteine endopeptidase; AUTL1; AUTL3; Autophagin 3; Autophagin-3; Autophagy related 4C cysteine peptidase; Autophagy related cysteine endopeptidase 3; Autophagy related protein 4 homolog C; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; Cysteine protease ATG4C; EC 3.4.22; FLJ14867; OTTHUMP00000010715;
Immunogens
- Q96DT6 ATG4C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEATGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDEDKTLPAESGCTIEDHVIAGNVEEFRKDFISRIWLTYREEFPQIEGSALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALNIENSDSESWTSHTVKKFTASFEASLSGEREFKTPTISLKETIGKYSDDHEMRNEVYHRKIISWFGDSPLALFGLHQLIEYGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGITIYVAQDCTVYNSDVIDKQSASMTSDNADDKAVIILVPVRLGGERTNTDYLEFVKGILSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQSFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIGFYCRNVQDFKRASEEITKMLKFSSKEKYPLFTFVNGHSRDYDFTSTTTNEEDLFSEDEKKQLKRFSTEEFVLL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96DT6 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| Y25 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| Y185 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| T452 | Phosphorylation | Uniprot |
Research Backgrounds
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Cytoplasm.
Highly expressed in skeletal muscle, heart, liver and testis.
Belongs to the peptidase C54 family.
Research Fields
· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
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