ELOVL5 Antibody - #DF4038

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Product: ELOVL5 Antibody
Catalog: DF4038
Description: Rabbit polyclonal antibody to ELOVL5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 28 KD; 35kD(Calculated).
Uniprot: Q9NYP7
RRID: AB_2836331

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(89%), Bovine(90%), Sheep(90%), Rabbit(90%), Dog(90%)
Clonality:
Polyclonal
Specificity:
ELOVL5 Antibody detects endogenous levels of total ELOVL5.
RRID:
AB_2836331
Cite Format: Affinity Biosciences Cat# DF4038, RRID:AB_2836331.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3 keto acyl CoA synthase ELOVL5; 3-keto acyl-CoA synthase Elovl5; dJ483K16.1; Elongation of very long chain fatty acids like 5; Elongation of very long chain fatty acids protein 5; ELOV5_HUMAN; ELOVL 5; ELOVL family member 5; ELOVL family member 5 elongation of long chain fatty acids; ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast); ELOVL fatty acid elongase 5; ELOVL2; elovl5; Fatty acid elongase 1; hELO1; Homolog of yeast long chain polyunsaturated fatty acid elongation enzyme 2; OTTHUMP00000017867; RP3 483K16.1; RP3-483K16.1;

Immunogens

Immunogen:
Uniprot:

Q9NYP7(ELOV5_HUMAN) >>Visit HPA database.

Gene(ID):
ELOVL5(60481)
Expression:
Q9NYP7 ELOV5_HUMAN:

Ubiquitous. Highly expressed in the adrenal gland and testis. Weakly expressed in prostate, lung and brain. Expressed in the cerebellum.

Sequence:
MEHFDASLSTYFKALLGPRDTRVKGWFLLDNYIPTFICSVIYLLIVWLGPKYMRNKQPFSCRGILVVYNLGLTLLSLYMFCELVTGVWEGKYNFFCQGTRTAGESDMKIIRVLWWYYFSKLIEFMDTFFFILRKNNHQITVLHVYHHASMLNIWWFVMNWVPCGHSYFGATLNSFIHVLMYSYYGLSSVPSMRPYLWWKKYITQGQLLQFVLTIIQTSCGVIWPCTFPLGWLYFQIGYMISLIALFTNFYIQTYNKKGASRRKDHLKDHQNGSMAAVNGHTNSFSPLENNVKPRKLRKD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
90
Sheep
90
Dog
90
Rabbit
90
Pig
89
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NYP7 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K13 Ubiquitination
K56 Ubiquitination
K267 Ubiquitination
S273 Phosphorylation
T281 Phosphorylation
S283 Phosphorylation
S285 Phosphorylation

Research Backgrounds

Function:

Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. May participate in the production of monounsaturated and of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators (By similarity). In conditions where the essential linoleic and alpha linoleic fatty acids are lacking it is also involved in the synthesis of Mead acid from oleic acid (By similarity).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Cell projection>Dendrite.
Note: In Purkinje cells, the protein localizes to the soma and proximal portion of the dendritic tree.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Highly expressed in the adrenal gland and testis. Weakly expressed in prostate, lung and brain. Expressed in the cerebellum.

Family&Domains:

Belongs to the ELO family. ELOVL5 subfamily.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid elongation.

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Fatty acid metabolism.

Restrictive clause

 

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