HSD11B1 Antibody - #DF3972

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Product: HSD11B1 Antibody
Catalog: DF3972
Description: Rabbit polyclonal antibody to HSD11B1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 32 KD; 32kD(Calculated).
Uniprot: P28845
RRID: AB_2836325

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
HSD11B1 Antibody detects endogenous levels of total HSD11B1.
RRID:
AB_2836325
Cite Format: Affinity Biosciences Cat# DF3972, RRID:AB_2836325.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

11 beta HSD 1; 11 beta HSD1; 11 beta hydroxysteroid dehydrogenase 1; 11 DH; 11-beta hydroxysteroid dehydrogenase, type 1; 11-beta-HSD1; 11-beta-hydroxysteroid dehydrogenase 1; 11-DH; 11DH; Corticosteroid 11 beta dehydrogenase isozyme 1; Corticosteroid 11-beta-dehydrogenase isozyme 1; CORTRD2; DHI1_HUMAN; HDL; HSD 11; HSD11; HSD11B; HSD11B1; HSD11L; Hydroxysteroid (11 beta) dehydrogenase; Hydroxysteroid (11 beta) dehydrogenase 1; MGC13539; SDR26C1; short chain dehydrogenase/reductase family 26C, member 1;

Immunogens

Immunogen:
Uniprot:

P28845(DHI1_HUMAN) >>Visit HPA database.

Gene(ID):
HSD11B1(3290)
Expression:
P28845 DHI1_HUMAN:

Widely expressed. Highest expression in liver.

Sequence:
MAFMKKYLLPILGLFMAYYYYSANEEFRPEMLQGKKVIVTGASKGIGREMAYHLAKMGAHVVVTARSKETLQKVVSHCLELGAASAHYIAGTMEDMTFAEQFVAQAGKLMGGLDMLILNHITNTSLNLFHDDIHHVRKSMEVNFLSYVVLTVAALPMLKQSNGSIVVVSSLAGKVAYPMVAAYSASKFALDGFFSSIRKEYSVSRVNVSITLCVLGLIDTETAMKAVSGIVHMQAAPKEECALEIIKGGALRQEEVYYDSSLWTTLLIRNPCRKILEFLYSTSYNMDRFINK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
55
Pig
0
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P28845 As Substrate

Site PTM Type Enzyme
S22 Phosphorylation
K56 Methylation
N123 N-Glycosylation
N162 N-Glycosylation
S196 Phosphorylation

Research Backgrounds

Function:

Catalyzes reversibly the conversion of cortisol to the inactive metabolite cortisone. Catalyzes reversibly the conversion of 7-ketocholesterol to 7-beta-hydroxycholesterol. In intact cells, the reaction runs only in one direction, from 7-ketocholesterol to 7-beta-hydroxycholesterol (By similarity).

PTMs:

Glycosylated.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type II membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Highest expression in liver.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Sp1 participates in the cadmium-induced imbalance of the placental glucocorticoid barrier by suppressing 11β-HSD2 expression. ENVIRONMENTAL POLLUTION (PubMed: 32044612) [IF=8.9]

Application: WB    Species: rat    Sample: placenta

Fig. 4. Effects of Cd on 11b-HSD1/11b-HSD2/Sp1 expression. (AeD) RT-PCR and WB of 11b-HSD1/11b-HSD2/Sp1 mRNA and protein expression, n ¼ 10. (EeH) ChIP of Sp1 and p300 enrichment to the 11b-HSD2 promoter, n test (EeH). (mean ± S.E.M.,*P < 0.05,**P <¼ 0.01 compare to NP group). 3. The difference between two groups was conducted by Student’s t-test (BeD), Frequency measures were assessed by the chi-square

Application: IF/ICC    Species: rat    Sample: placenta

Fig. 3. Immunofluorescence of 11b-HSD1 and 11b-HSD2 in placenta and their optical density (magnification of 400  ), n ¼ 3. The difference between two groups was conducted by Student’s t-test. (mean ± S.E.M.,**P < 0.01 compare to NP group).

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