CCS/SOD4 Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

1/4

DF3971 at 1/100 staining Human pancreatic cancer by IHC-P.

DF3971 at 1/100 staining Rat heart tissue by IHC-P.

DF3971 staining HepG2 cells by IF/ICC.

Fig.

Product:	CCS/SOD4 Antibody
Catalog:	DF3971
Description:	Rabbit polyclonal antibody to CCS/SOD4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit
Mol.Wt.:	32 KD; 29kD(Calculated).
Uniprot:	O14618
RRID:	AB_2836324

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(80%), Horse(90%), Sheep(80%), Rabbit(90%)

Clonality:

Polyclonal

Specificity:

CCS/SOD4 Antibody detects endogenous levels of total CCS/SOD4.

RRID:

AB_2836324
Cite Format: Affinity Biosciences Cat# DF3971, RRID:AB_2836324.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCS; CCS_HUMAN; Copper chaperone for superoxide dismutase; MGC138260; SOD 4; SOD4; Superoxide dismutase copper chaperone; Superoxide Dismutase 4;

Immunogens

Immunogen:

Uniprot:

O14618(CCS_HUMAN) >>Visit HPA database.

Gene(ID):

CCS(9973)

Expression:

O14618 CCS_HUMAN:

Ubiquitous.

Sequence:

O14618 CCS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASDSGNQGTLCTLEFAVQMTCQSCVDAVRKSLQGVAGVQDVEVHLEDQMVLVHTTLPSQEVQALLEGTGRQAVLKGMGSGQLQNLGAAVAILGGPGTVQGVVRFLQLTPERCLIEGTIDGLEPGLHGLHVHQYGDLTNNCNSCGNHFNPDGASHGGPQDSDRHRGDLGNVRADADGRAIFRMEDEQLKVWDVIGRSLIIDEGEDDLGRGGHPLSKITGNSGERLACGIIARSAGLFQNPKQICSCDGLTIWEERGRPIAGKGRKESAQPPAHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Horse

Pig

Sheep

Dog

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14618 As Substrate

O14618

Site	PTM Type	Source
K76	Ubiquitination	Uniprot
R165	Methylation	Uniprot
K189	Ubiquitination	Uniprot
R209	Methylation	Uniprot
K216	Ubiquitination	Uniprot
K241	Ubiquitination	Uniprot
S267	Phosphorylation	Uniprot

Research Backgrounds

O14618_CCS_HUMAN

Function:

Delivers copper to copper zinc superoxide dismutase (SOD1).

PTMs:

Ubiquitinion by XIAP/BIRC4 leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. XIAP/BIRC4 preferentially ubiquitinates at Lys-241.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer, and heterodimer with SOD1. Interacts with COMMD1. Interacts with XIAP/BIRC4.

Family&Domains:

In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

References

1). Metformin-based carbon dots based on biguanide functional groups for simultaneous chelation of copper ions and inhibitable colorectal cancer therapy. Carbon, 2023 [IF=10.9]

2). Inhibition of ASIC1a-Mediated ERS Improves the Activation of HSCs and Copper Transport Under Copper Load. Frontiers in Pharmacology, 2021 (PubMed: 34135753) [IF=5.6]

3). Associations of polymorphisms in LOXL1 and copper chaperone genes with pseudoexfoliation-syndrome-related cataract in a Chinese Uygur population. INTERNATIONAL OPHTHALMOLOGY, 2020 (PubMed: 32253563) [IF=1.6]

Application: WB Species: human Sample: lens capsule

Fig. 1 |CCS expression levels. a Western blotting of the CCS protein in the lens capsule. CCS expression was greater in the PEXC group than in the ARC group

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CCS/SOD4 Antibody - #DF3971