CCS/SOD4 Antibody - #DF3971

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Product: CCS/SOD4 Antibody
Catalog: DF3971
Description: Rabbit polyclonal antibody to CCS/SOD4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit
Mol.Wt.: 32 KD; 29kD(Calculated).
Uniprot: O14618
RRID: AB_2836324

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Horse(90%), Sheep(80%), Rabbit(90%)
Clonality:
Polyclonal
Specificity:
CCS/SOD4 Antibody detects endogenous levels of total CCS/SOD4.
RRID:
AB_2836324
Cite Format: Affinity Biosciences Cat# DF3971, RRID:AB_2836324.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCS; CCS_HUMAN; Copper chaperone for superoxide dismutase; MGC138260; SOD 4; SOD4; Superoxide dismutase copper chaperone; Superoxide Dismutase 4;

Immunogens

Immunogen:
Uniprot:

O14618(CCS_HUMAN) >>Visit HPA database.

Gene(ID):
CCS(9973)
Expression:
O14618 CCS_HUMAN:

Ubiquitous.

Sequence:
MASDSGNQGTLCTLEFAVQMTCQSCVDAVRKSLQGVAGVQDVEVHLEDQMVLVHTTLPSQEVQALLEGTGRQAVLKGMGSGQLQNLGAAVAILGGPGTVQGVVRFLQLTPERCLIEGTIDGLEPGLHGLHVHQYGDLTNNCNSCGNHFNPDGASHGGPQDSDRHRGDLGNVRADADGRAIFRMEDEQLKVWDVIGRSLIIDEGEDDLGRGGHPLSKITGNSGERLACGIIARSAGLFQNPKQICSCDGLTIWEERGRPIAGKGRKESAQPPAHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Horse
90
Pig
80
Sheep
80
Dog
70
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14618 As Substrate

Site PTM Type Enzyme
K76 Ubiquitination
R165 Methylation
K189 Ubiquitination
R209 Methylation
K216 Ubiquitination
K241 Ubiquitination
S267 Phosphorylation

Research Backgrounds

Function:

Delivers copper to copper zinc superoxide dismutase (SOD1).

PTMs:

Ubiquitinion by XIAP/BIRC4 leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. XIAP/BIRC4 preferentially ubiquitinates at Lys-241.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer, and heterodimer with SOD1. Interacts with COMMD1. Interacts with XIAP/BIRC4.

Family&Domains:

In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

References

1). Metformin-based carbon dots based on biguanide functional groups for simultaneous chelation of copper ions and inhibitable colorectal cancer therapy. Carbon [IF=10.9]
2). Associations of polymorphisms in LOXL1 and copper chaperone genes with pseudoexfoliation-syndrome-related cataract in a Chinese Uygur population. INTERNATIONAL OPHTHALMOLOGY (PubMed: 32253563) [IF=1.6]

Application: WB    Species: human    Sample: lens capsule

Fig. 1 |CCS expression levels. a Western blotting of the CCS protein in the lens capsule. CCS expression was greater in the PEXC group than in the ARC group

Restrictive clause

 

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For Research Use Only.
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