NCAPG Antibody - #DF3968

(1)  
Product: NCAPG Antibody
Catalog: DF3968
Description: Rabbit polyclonal antibody to NCAPG
Application: WB IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 114 KD; 114kD(Calculated).
Uniprot: Q9BPX3
RRID: AB_2836321

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
NCAPG Antibody detects endogenous levels of total NCAPG.
RRID:
AB_2836321
Cite Format: Affinity Biosciences Cat# DF3968, RRID:AB_2836321.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAPG; CHCG; Chromosome associated protein G; Chromosome condensation protein G; Condensin complex subunit 3; Condensin subunit CAP G; HCAP G; Melanoma antigen NY MEL 3; NCAPG; Non SMC condensin I complex subunit G; NY MEL 3; XCAP G homolog;

Immunogens

Immunogen:
Uniprot:

Q9BPX3(CND3_HUMAN) >>Visit HPA database.

Gene(ID):
NCAPG(64151)
Expression:
Q9BPX3 CND3_HUMAN:

Highly expressed in testis.

Sequence:
MGAERRLLSIKEAFRLAQQPHQNQAKLVVALSRTYRTMDDKTVFHEEFIHYLKYVMVVYKREPAVERVIEFAAKFVTSFHQSDMEDDEEEEDGGLLNYLFTFLLKSHEANSNAVRFRVCLLINKLLGSMPENAQIDDDVFDKINKAMLIRLKDKIPNVRIQAVLALSRLQDPKDDECPVVNAYATLIENDSNPEVRRAVLSCIAPSAKTLPKIVGRTKDVKEAVRKLAYQVLAEKVHMRAMSIAQRVMLLQQGLNDRSDAVKQAMQKHLLQGWLRFSEGNILELLHRLDVENSSEVAVSVLNALFSITPLSELVGLCKNNDGRKLIPVETLTPEIALYWCALCEYLKSKGDEGEEFLEQILPEPVVYADYLLSYIQSIPVVNEEHRGDFSYIGNLMTKEFIGQQLILIIKSLDTSEEGGRKKLLAVLQEILILPTIPISLVSFLVERLLHIIIDDNKRTQIVTEIISEIRAPIVTVGVNNDPADVRKKELKMAEIKVKLIEAKEALENCITLQDFNRASELKEEIKALEDARINLLKETEQLEIKEVHIEKNDAETLQKCLILCYELLKQMSISTGLSATMNGIIESLILPGIISIHPVVRNLAVLCLGCCGLQNQDFARKHFVLLLQVLQIDDVTIKISALKAIFDQLMTFGIEPFKTKKIKTLHCEGTEINSDDEQESKEVEETATAKNVLKLLSDFLDSEVSELRTGAAEGLAKLMFSGLLVSSRILSRLILLWYNPVTEEDVQLRHCLGVFFPVFAYASRTNQECFEEAFLPTLQTLANAPASSPLAEIDITNVAELLVDLTRPSGLNPQAKTSQDYQALTVHDNLAMKICNEILTSPCSPEIRVYTKALSSLELSSHLAKDLLVLLNEILEQVKDRTCLRALEKIKIQLEKGNKEFGDQAEAAQDATLTTTTFQNEDEKNKEVYMTPLRGVKATQASKSTQLKTNRGQRKVTVSARTNRRCQTAEADSESDHEVPEPESEMKMRLPRRAKTAALEKSKLNLAQFLNEDLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BPX3 As Substrate

Site PTM Type Enzyme
Phosphorylation
K11 Ubiquitination
K26 Ubiquitination
S32 Phosphorylation
K142 Ubiquitination
K145 Ubiquitination
K152 Ubiquitination
K154 Ubiquitination
S167 Phosphorylation
S206 Phosphorylation
K208 Ubiquitination
K212 Ubiquitination
K226 Ubiquitination
K235 Ubiquitination
R257 Methylation
K262 Ubiquitination
K267 Ubiquitination
T308 Phosphorylation P06493 (CDK1)
T332 Phosphorylation P06493 (CDK1)
S390 Phosphorylation
T397 Phosphorylation
K410 Ubiquitination
T463 Phosphorylation
S467 Phosphorylation
K491 Acetylation
K496 Ubiquitination
K498 Ubiquitination
K503 Ubiquitination
K522 Ubiquitination
K526 Ubiquitination
K537 Ubiquitination
K545 Ubiquitination
K551 Ubiquitination
T664 Phosphorylation
T670 Phosphorylation
S674 Phosphorylation
S680 Phosphorylation
T686 Phosphorylation
K690 Ubiquitination
S697 Phosphorylation
S705 Phosphorylation
K717 Ubiquitination
T817 Phosphorylation
S818 Phosphorylation
Y821 Phosphorylation
K833 Ubiquitination
S841 Phosphorylation
S844 Phosphorylation
T851 Phosphorylation
K852 Ubiquitination
K891 Ubiquitination
K896 Ubiquitination
K899 Ubiquitination
Y929 Phosphorylation
T931 Phosphorylation P06493 (CDK1)
R934 Methylation
K937 Ubiquitination
K943 Ubiquitination
S944 Phosphorylation
T945 Phosphorylation
S959 Phosphorylation
T968 Phosphorylation
S973 Phosphorylation P68400 (CSNK2A1)
S975 Phosphorylation P68400 (CSNK2A1)
S984 Phosphorylation
T996 Phosphorylation
S1002 Phosphorylation
K1003 Ubiquitination
S1015 Phosphorylation

Research Backgrounds

Function:

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

PTMs:

Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPH subunits, activates the condensin complex and is required for chromosome condensation (By similarity).

Subcellular Location:

Nucleus. Cytoplasm. Chromosome.
Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis.

Subunit Structure:

Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: NCAPH/BRRN1, NCAPD2/CAPD2 and NCAPG.

Family&Domains:

Belongs to the CND3 (condensin subunit 3) family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.