NCAPH Antibody | Affinity Biosciences

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NCAPH Antibody - Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using NCAPH Antibody at 1/1000 dilution.

NCAPH Antibody - Figure 2.

Product:	NCAPH Antibody
Catalog:	DF3967
Description:	Rabbit polyclonal antibody to NCAPH
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Horse, Rabbit
Mol.Wt.:	83 KD; 83kD(Calculated).
Uniprot:	Q15003
RRID:	AB_2836320

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(83%), Horse(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

NCAPH Antibody detects endogenous levels of total NCAPH.

RRID:

AB_2836320
Cite Format: Affinity Biosciences Cat# DF3967, RRID:AB_2836320.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Barren Drosophila Homolog of 1; Barren homolog protein 1; BRRN; BRRN1; CAP H; CAPH; Chromosome associated protein H; Chromosome-associated protein H; CND2_HUMAN; Condensin complex subunit 2; Condensin subunit 2; Condensin-2 complex subunit H2; Condesin; HCAP H; hCAP-H; KIAA0074; mCAP H; MGC4616; Ncaph; Non SMC condensin I complex subunit H; Non-SMC condensin I complex subunit H; XCAP H homolog; XCAP-H homolog;

Immunogens

Immunogen:

Uniprot:

Q15003(CND2_HUMAN) >>Visit HPA database.

Gene(ID):

NCAPH(23397)

Expression:

Q15003 CND2_HUMAN:

Widely expressed at low level. Expressed in proliferating cells.

Sequence:

Q15003 CND2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGPPGPALPATMNNSSSETRGHPHSASSPSERVFPMPLPRKAPLNIPGTPVLEDFPQNDDEKERLQRRRSRVFDLQFSTDSPRLLASPSSRSIDISATIPKFTNTQITEHYSTCIKLSTENKITTKNAFGLHLIDFMSEILKQKDTEPTNFKVAAGTLDASTKIYAVRVDAVHADVYRVLGGLGKDAPSLEEVEGHVADGSATEMGTTKKAVKPKKKHLHRTIEQNINNLNVSEADRKCEIDPMFQKTAASFDECSTAGVFLSTLHCQDYRSELLFPSDVQTLSTGEPLELPELGCVEMTDLKAPLQQCAEDRQICPSLAGFQFTQWDSETHNESVSALVDKFKKNDQVFDINAEVDESDCGDFPDGSLGDDFDANDEPDHTAVGDHEEFRSWKEPCQVQSCQEEMISLGDGDIRTMCPLLSMKPGEYSYFSPRTMSMWAGPDHWRFRPRRKQDAPSQSENKKKSTKKDFEIDFEDDIDFDVYFRKTKAATILTKSTLENQNWRATTLPTDFNYNVDTLVQLHLKPGTRLLKMAQGHRVETEHYEEIEDYDYNNPNDTSNFCPGLQAADSDDEDLDDLFVGPVGNSDLSPYPCHPPKTAQQNGDTPEAQGLDITTYGESNLVAEPQKVNKIEIHYAKTAKKMDMKKLKQSMWSLLTALSGKEADAEANHREAGKEAALAEVADEKMLSGLTKDLQRSLPPVMAQNLSIPLAFACLLHLANEKNLKLEGTEDLSDVLVRQGD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

100

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15003 As Substrate

Q15003

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
S15	Phosphorylation		Uniprot
S16	Phosphorylation		Uniprot
S17	Phosphorylation		Uniprot
R20	Methylation		Uniprot
S25	Phosphorylation		Uniprot
S27	Phosphorylation		Uniprot
S28	Phosphorylation		Uniprot
S30	Phosphorylation		Uniprot
R32	Methylation		Uniprot
K41	Ubiquitination		Uniprot
T49	Phosphorylation	P24941 (CDK2)	Uniprot
K62	Ubiquitination		Uniprot
S70	Phosphorylation		Uniprot
S78	Phosphorylation		Uniprot
T79	Phosphorylation		Uniprot
S81	Phosphorylation		Uniprot
S87	Phosphorylation		Uniprot
S89	Phosphorylation		Uniprot
S90	Phosphorylation		Uniprot
S92	Phosphorylation		Uniprot
S96	Phosphorylation		Uniprot
T98	Phosphorylation		Uniprot
Y111	Phosphorylation		Uniprot
K122	Ubiquitination		Uniprot
K126	Ubiquitination		Uniprot
K142	Ubiquitination		Uniprot
K144	Ubiquitination		Uniprot
T146	Phosphorylation		Uniprot
K152	Methylation		Uniprot
K152	Ubiquitination		Uniprot
T157	Phosphorylation		Uniprot
K163	Ubiquitination		Uniprot
Y165	Phosphorylation		Uniprot
Y177	Phosphorylation		Uniprot
K185	Acetylation		Uniprot
K185	Ubiquitination		Uniprot
S189	Phosphorylation		Uniprot
S201	Phosphorylation		Uniprot
T203	Phosphorylation		Uniprot
T207	Phosphorylation		Uniprot
S233	Phosphorylation		Uniprot
K238	Ubiquitination		Uniprot
S335	Phosphorylation		Uniprot
K394	Ubiquitination		Uniprot
S401	Phosphorylation		Uniprot
S408	Phosphorylation		Uniprot
K424	Ubiquitination		Uniprot
Y428	Phosphorylation		Uniprot
S429	Phosphorylation		Uniprot
Y430	Phosphorylation		Uniprot
S432	Phosphorylation		Uniprot
K488	Ubiquitination		Uniprot
K495	Ubiquitination		Uniprot
S496	Phosphorylation		Uniprot
Y514	Phosphorylation		Uniprot
K532	Ubiquitination		Uniprot
S570	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S589	Phosphorylation		Uniprot
T598	Phosphorylation		Uniprot
T605	Phosphorylation		Uniprot
T614	Phosphorylation		Uniprot
Y616	Phosphorylation		Uniprot
K630	Ubiquitination		Uniprot
Y635	Phosphorylation		Uniprot
K637	Acetylation		Uniprot
K637	Ubiquitination		Uniprot
S653	Phosphorylation		Uniprot
K661	Ubiquitination		Uniprot
K674	Acetylation		Uniprot
K674	Ubiquitination		Uniprot
K685	Ubiquitination		Uniprot
K692	Acetylation		Uniprot
K692	Ubiquitination		Uniprot
K725	Sumoylation		Uniprot
K725	Ubiquitination		Uniprot
S733	Phosphorylation		Uniprot

Research Backgrounds

Q15003_CND2_HUMAN

Function:

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.

PTMs:

Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPG subunits, activates the condensin complex and is required for chromosome condensation (By similarity).

Subcellular Location:

Nucleus. Cytoplasm. Chromosome.
Note: In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.

Tissue Specificity:

Widely expressed at low level. Expressed in proliferating cells.

Subunit Structure:

Component of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex: NCAPH/BRRN1, NCAPD2/CAPD2 and NCAPG.

Family&Domains:

Belongs to the CND2 (condensin subunit 2) family.

References

1). NCAPH promotes cell proliferation and inhibits cell apoptosis of bladder cancer cells through MEK/ERK signaling pathway. CELL CYCLE, 2022 (PubMed: 34974790) [IF=3.4]

Application: WB Species: Mouse Sample: SW780 cells

Figure 2. NCAPH promotes the cell proliferation of BC cells. (a,b) The mRNA and protein levels of NCAPH in SW780 cells transfected with NCAPH expression vector and control vector were determined by qRT‐PCR and Western blot. (c,d) The mRNA and protein levels of NCAPH in UMUC3 cells transfected with NCAPH siRNA1-3 and negative control (siNC) were determined by qRT‐PCR and Western blot. (e-h) The effect of NCAPH on cell proliferation of BC cells was determined by BrdU assay (Scale bar, 100 μm) and MTT assay. The data were presented as the mean ± SD.

Application: IF/ICC Species: Mouse Sample: SW780 cells

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NCAPH Antibody - #DF3967