Factor I/CFI Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat cells, using CFI antibody.

Product:	Factor I/CFI Antibody
Catalog:	DF3966
Description:	Rabbit polyclonal antibody to Factor I/CFI
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Dog
Mol.Wt.:	66 KD; 66kD(Calculated).
Uniprot:	P05156
RRID:	AB_2836319

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(80%), Zebrafish(83%), Bovine(80%), Dog(80%)

Clonality:

Polyclonal

Specificity:

Factor I/CFI Antibody detects endogenous levels of total Factor I/CFI.

RRID:

AB_2836319
Cite Format: Affinity Biosciences Cat# DF3966, RRID:AB_2836319.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AHUS3; ARMD13; C3b INA; C3b inactivator; C3B/C4B inactivator; C3BINA; CFAI_HUMAN; Cfi; Complement component I; Complement control protein factor I; Complement factor I; Complement factor I heavy chain; Complement factor I light chain; F1; factor I; FactorI; FI; I factor; IF; KAF; Konglutinogen activating factor; Light chain of factor I; OTTHUMP00000219728; OTTHUMP00000221928;

Immunogens

Immunogen:

Uniprot:

P05156(CFAI_HUMAN) >>Visit HPA database.

Gene(ID):

CFI(3426)

Expression:

P05156 CFAI_HUMAN:

Expressed in the liver by hepatocytes (PubMed:6327681). Also present in other cells such as monocytes, fibroblasts or keratinocytes (PubMed:6444659, PubMed:17320177).

Sequence:

P05156 CFAI_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKLLHVFLLFLCFHLRFCKVTYTSQEDLVEKKCLAKKYTHLSCDKVFCQPWQRCIEGTCVCKLPYQCPKNGTAVCATNRRSFPTYCQQKSLECLHPGTKFLNNGTCTAEGKFSVSLKHGNTDSEGIVEVKLVDQDKTMFICKSSWSMREANVACLDLGFQQGADTQRRFKLSDLSINSTECLHVHCRGLETSLAECTFTKRRTMGYQDFADVVCYTQKADSPMDDFFQCVNGKYISQMKACDGINDCGDQSDELCCKACQGKGFHCKSGVCIPSQYQCNGEVDCITGEDEVGCAGFASVTQEETEILTADMDAERRRIKSLLPKLSCGVKNRMHIRRKRIVGGKRAQLGDLPWQVAIKDASGITCGGIYIGGCWILTAAHCLRASKTHRYQIWTTVVDWIHPDLKRIVIEYVDRIIFHENYNAGTYQNDIALIEMKKDGNKKDCELPRSIPACVPWSPYLFQPNDTCIVSGWGREKDNERVFSLQWGEVKLISNCSKFYGNRFYEKEMECAGTYDGSIDACKGDSGGPLVCMDANNVTYVWGVVSWGENCGKPEFPGVYTKVANYFDWISYHVGRPFISQYNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Zebrafish

Pig

Bovine

Dog

Sheep

Xenopus

Horse

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05156 As Substrate

P05156

Site	PTM Type	Source
Y22	Phosphorylation	Uniprot
N70	N-Glycosylation	Uniprot
S90	Phosphorylation	Uniprot
N103	N-Glycosylation	Uniprot
N177	N-Glycosylation	Uniprot
Y206	Phosphorylation	Uniprot
T425	Phosphorylation	Uniprot
N464	N-Glycosylation	Uniprot
S483	Phosphorylation	Uniprot
N494	N-Glycosylation	Uniprot
N536	N-Glycosylation	Uniprot

Research Backgrounds

P05156_CFAI_HUMAN

Function:

Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins. Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces. The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization.

Subcellular Location:

Secreted>Extracellular space. Secreted.

Tissue Specificity:

Expressed in the liver by hepatocytes. Also present in other cells such as monocytes, fibroblasts or keratinocytes.

Subunit Structure:

Heterodimer of a light and heavy chains; disulfide-linked. The fully processed and mature protein circulates as a zymogen, and is allosterically activated by substrate-induced remodeling of the active site. Interacts with C3b. Interacts with complement factor H.

(Microbial infection) Interacts with Staphylococcus aureus clumping factor A/ClfA; this interaction enhances cleavage of C3b into iC3b by CFI.

Family&Domains:

Belongs to the peptidase S1 family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

Restrictive clause

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For Research Use Only.
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Factor I/CFI Antibody - #DF3966