ST6GAL1 Antibody - #DF3950

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Product: ST6GAL1 Antibody
Catalog: DF3950
Description: Rabbit polyclonal antibody to ST6GAL1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 42 KD，55KD(Glycosylation); 47kD(Calculated).
Uniprot: P15907
RRID: AB_2836303

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(82%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
ST6GAL1 Antibody detects endogenous levels of total ST6GAL1.
RRID:
AB_2836303
Cite Format: Affinity Biosciences Cat# DF3950, RRID:AB_2836303.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

6 sialyltransferase; 6-sialyltransferase 1; 6-ST 1; Alpha 2; Alpha 2,6 ST 1; Alpha 2,6 ST; B cell antigen CD75; B-cell antigen CD75; Beta galactoside alpha 2,6 sialyltransferase 1; Beta-galactoside alpha-2; CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2; MGC48859; Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase); Sialyltransferase 1; SIAT1; SIAT1_HUMAN; ST6 beta galactosamide alpha 2,6 sialyltranferase 1; ST6Gal I; ST6GAL1; ST6GalI; ST6N;

Immunogens

Immunogen:
Uniprot:

P15907(SIAT1_HUMAN) >>Visit HPA database.

Gene(ID):
ST6GAL1(6480)
Sequence:
MIHTNLKKKFSCCVLVFLLFAVICVWKEKKKGSYYDSFKLQTKEFQVLKSLGKLAMGSDSQSVSSSSTQDPHRGRQTLGSLRGLAKAKPEASFQVWNKDSSSKNLIPRLQKIWKNYLSMNKYKVSYKGPGPGIKFSAEALRCHLRDHVNVSMVEVTDFPFNTSEWEGYLPKESIRTKAGPWGRCAVVSSAGSLKSSQLGREIDDHDAVLRFNGAPTANFQQDVGTKTTIRLMNSQLVTTEKRFLKDSLYNEGILIVWDPSVYHSDIPKWYQNPDYNFFNNYKTYRKLHPNQPFYILKPQMPWELWDILQEISPEEIQPNPPSSGMLGIIIMMTLCDQVDIYEFLPSKRKTDVCYYYQKFFDSACTMGAYHPLLYEKNLVKHLNQGTDEDIYLLGKATLPGFRTIHC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Pig
91
Horse
91
Rabbit
91
Zebrafish
82
Chicken
82
Xenopus
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15907 As Substrate

Site PTM Type Enzyme
T68 O-Glycosylation
T77 Phosphorylation
S80 Phosphorylation
S92 Phosphorylation
N149 N-Glycosylation
N161 N-Glycosylation
K177 Ubiquitination
K194 Ubiquitination
K226 Ubiquitination
K241 Ubiquitination
Y369 Phosphorylation
K380 Ubiquitination

Research Backgrounds

Function:

Transfers sialic acid from CMP-sialic acid to galactose-containing acceptor substrates.

PTMs:

The soluble form derives from the membrane form by proteolytic processing.

The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.

N-glycosylated.

Subcellular Location:

Golgi apparatus>Golgi stack membrane>Single-pass type II membrane protein. Secreted.
Note: Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer and homodimer.

Family&Domains:

Belongs to the glycosyltransferase 29 family.

Research Fields

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Glycan biosynthesis and metabolism > Other types of O-glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Intranasal mask for protecting the respiratory tract against viral aerosols. Nature communications (PubMed: 38110357) [IF=16.6]

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