Claudin 8 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from A2780 cells, using CLDN8 Antibody.

Product:	Claudin 8 Antibody
Catalog:	DF3947
Description:	Rabbit polyclonal antibody to Claudin 8
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	28 KD; 25kD(Calculated).
Uniprot:	P56748
RRID:	AB_2836300

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(82%), Horse(82%), Sheep(91%), Rabbit(82%)

Clonality:

Polyclonal

Specificity:

Claudin 8 Antibody detects endogenous levels of total Claudin 8.

RRID:

AB_2836300
Cite Format: Affinity Biosciences Cat# DF3947, RRID:AB_2836300.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Claudin 8; Claudin-8; CLD8_HUMAN; Cldn8; Epididymis secretory protein Li 79; HEL S 79; OTTHUMP00000101915;

Immunogens

Immunogen:

Uniprot:

P56748(CLD8_HUMAN) >>Visit HPA database.

Gene(ID):

CLDN8(9073)

Expression:

P56748 CLD8_HUMAN:

Expressed in the epididymis, mainly in the caput segment.

Sequence:

P56748 CLD8_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATHALEIAGLFLGGVGMVGTVAVTVMPQWRVSAFIENNIVVFENFWEGLWMNCVRQANIRMQCKIYDSLLALSPDLQAARGLMCAASVMSFLAFMMAILGMKCTRCTGDNEKVKAHILLTAGIIFIITGMVVLIPVSWVANAIIRDFYNSIVNVAQKRELGEALYLGWTTALVLIVGGALFCCVFCCNEKSSSYRYSIPSHRTTQKSYHTGKKSPSVYSRSQYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Sheep

Horse

Bovine

Rabbit

Pig

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56748 As Substrate

P56748

Site	PTM Type	Source
Y67	Phosphorylation	Uniprot
S69	Phosphorylation	Uniprot
S74	Phosphorylation	Uniprot
Y197	Phosphorylation	Uniprot
S198	Phosphorylation	Uniprot
T211	Phosphorylation	Uniprot
S215	Phosphorylation	Uniprot
S217	Phosphorylation	Uniprot

Research Backgrounds

P56748_CLD8_HUMAN

Function:

Tight-junction protein required for paracellular chloride transport in the kidney. Mediates recruitment of CLDN4 to tight junction in the kidney. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

PTMs:

Ubiquitinated by the BCR(KLHL3) E3 ubiquitin ligase complex in the kidney, leading to its degradation.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.
Note: Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.

Tissue Specificity:

Expressed in the epididymis, mainly in the caput segment.

Subunit Structure:

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3. Interacts with CLDN4. Interacts with KLHL3.

Family&Domains:

Belongs to the claudin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

Restrictive clause

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For Research Use Only.
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Claudin 8 Antibody - #DF3947