CNGA1 Antibody - #DF3929

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Product: CNGA1 Antibody
Catalog: DF3929
Description: Rabbit polyclonal antibody to CNGA1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 79 KD; 80kD(Calculated).
Uniprot: P29973
RRID: AB_2836282

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
CNGA1 Antibody detects endogenous levels of total CNGA1.
RRID:
AB_2836282
Cite Format: Affinity Biosciences Cat# DF3929, RRID:AB_2836282.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cGMP gated cation channel alpha 1; cGMP-gated cation channel alpha-1; CNCG; CNCG1; CNG 1; CNG channel alpha 1; CNG channel alpha-1; CNG-1; CNG1; Cnga1; CNGA1_HUMAN; Cyclic nucleotide gated cation channel 1; Cyclic nucleotide gated channel alpha 1; Cyclic nucleotide gated channel photoreceptor; Cyclic nucleotide-gated cation channel 1; Cyclic nucleotide-gated channel alpha-1; Cyclic nucleotide-gated channel; Interleukin 1 homologue; OTTHUMP00000218675; OTTHUMP00000223313; OTTHUMP00000223314; photoreceptor; RCNC1; RCNCa; RCNCalpha; Rod photoreceptor cGMP gated channel subunit alpha; Rod photoreceptor cGMP-gated channel subunit alpha; RP49;

Immunogens

Immunogen:
Uniprot:

P29973(CNGA1_HUMAN) >>Visit HPA database.

Gene(ID):
CNGA1(1259)
Expression:
P29973 CNGA1_HUMAN:

Rod cells in the retina.

Sequence:
MKLSMKNNIINTQQSFVTMPNVIVPDIEKEIRRMENGACSSFSEDDDSASTSEESENENPHARGSFSYKSLRKGGPSQREQYLPGAIALFNVNNSSNKDQEPEEKKKKKKEKKSKSDDKNENKNDPEKKKKKKDKEKKKKEEKSKDKKEEEKKEVVVIDPSGNTYYNWLFCITLPVMYNWTMVIARACFDELQSDYLEYWLILDYVSDIVYLIDMFVRTRTGYLEQGLLVKEELKLINKYKSNLQFKLDVLSLIPTDLLYFKLGWNYPEIRLNRLLRFSRMFEFFQRTETRTNYPNIFRISNLVMYIVIIIHWNACVFYSISKAIGFGNDTWVYPDINDPEFGRLARKYVYSLYWSTLTLTTIGETPPPVRDSEYVFVVVDFLIGVLIFATIVGNIGSMISNMNAARAEFQARIDAIKQYMHFRNVSKDMEKRVIKWFDYLWTNKKTVDEKEVLKYLPDKLRAEIAINVHLDTLKKVRIFADCEAGLLVELVLKLQPQVYSPGDYICKKGDIGREMYIIKEGKLAVVADDGVTQFVVLSDGSYFGEISILNIKGSKAGNRRTANIKSIGYSDLFCLSKDDLMEALTEYPDAKTMLEEKGKQILMKDGLLDLNIANAGSDPKDLEEKVTRMEGSVDLLQTRFARILAEYESMQQKLKQRLTKVEKFLKPLIDTEFSSIEGPGAESGPIDST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29973 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
T18 Phosphorylation
K143 Acetylation
K145 Acetylation
K147 Acetylation
K152 Acetylation
Y223 Phosphorylation
Y349 Phosphorylation
Y351 Phosphorylation
Y354 Phosphorylation
T473 Phosphorylation
K556 Methylation
K566 Methylation

Research Backgrounds

Function:

Subunit of the rod cyclic GMP-gated cation channel, which is involved in the final stage of the phototransduction pathway. When light hits rod photoreceptors, cGMP concentrations decrease causing rapid closure of CNGA1/CNGB1 channels and, therefore, hyperpolarization of the membrane potential.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Rod cells in the retina.

Subunit Structure:

Forms a heterotetramer with CNGB1 in a 3:1 ratio. May also form cyclic nucleotide-activated homotetrameric channels, that are efficiently activated by saturating cGMP, but poorly activated by saturating cAMP compared to the heterotetramer with CNGB1.

Family&Domains:

The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits.

Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA1 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Organismal Systems > Sensory system > Phototransduction.

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