CEP57 Antibody - #DF3919

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Product: CEP57 Antibody
Catalog: DF3919
Description: Rabbit polyclonal antibody to CEP57
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Dog
Mol.Wt.: 50 KD; 57kD(Calculated).
Uniprot: Q86XR8
RRID: AB_2836272

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Dog(100%)
Clonality:
Polyclonal
Specificity:
CEP57 Antibody detects endogenous levels of total CEP57.
RRID:
AB_2836272
Cite Format: Affinity Biosciences Cat# DF3919, RRID:AB_2836272.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Centrosomal protein 57kDa; Centrosomal protein of 57 kDa; Cep57; Cep57 protein; CEP57_HUMAN; FGF2 interacting protein; FGF2-interacting protein; KIAA0092; MVA2; PIG8; Proliferation inducing protein 8; Testis specific protein 57; Testis-specific protein 57; Translokin; TSP57;

Immunogens

Immunogen:
Uniprot:

Q86XR8(CEP57_HUMAN) >>Visit HPA database.

Gene(ID):
CEP57(9702)
Expression:
Q86XR8 CEP57_HUMAN:

Ubiquitous.

Sequence:
MAAASVSAASGSHLSNSFAEPSRSNGSMVRHSSSPYVVYPSDKPFLNSDLRRSPSKPTLAYPESNSRAIFSALKNLQDKIRRLELERIQAEESVKTLSRETIEYKKVLDEQIQERENSKNEESKHNQELTSQLLAAENKCNLLEKQLEYMRNMIKHAEMERTSVLEKQVSLERERQHDQTHVQSQLEKLDLLEQEYNKLTTMQALAEKKMQELEAKLHEEEQERKRMQAKAAELQTGLETNRLIFEDKATPCVPNARRIKKKKSKPPEKKSSRNYFGAQPHYRLCLGDMPFVAGKSTSPSHAVVANVQLVLHLMKQHSKALCNDRVINSIPLAKQVSSRGGKSKKLSVTPPSSNGINEELSEVLQTLQDEFGQMSFDHQQLAKLIQESPTVELKDKLECELEALVGRMEAKANQITKVRKYQAQLEKQKLEKQKKELKATKKTLDEERNSSSRSGITGTTNKKDFMKLRPGEKRRKNLQLLKDMQSIQNSLQSSSLCWDY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
73
Chicken
70
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86XR8 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
S15 Phosphorylation
S17 Phosphorylation
S32 Phosphorylation
S33 Phosphorylation
S34 Phosphorylation
Y36 Phosphorylation
K43 Ubiquitination
S53 Phosphorylation
S55 Phosphorylation
K56 Ubiquitination
T58 Phosphorylation
Y61 Phosphorylation
S64 Phosphorylation
S66 Phosphorylation
K79 Ubiquitination
K95 Ubiquitination
T96 Phosphorylation
T101 Phosphorylation
Y104 Phosphorylation
K105 Ubiquitination
K106 Ubiquitination
S118 Phosphorylation
K119 Ubiquitination
K124 Ubiquitination
K139 Ubiquitination
K145 Ubiquitination
K155 Ubiquitination
K167 Ubiquitination
K188 Ubiquitination
K198 Ubiquitination
K208 Ubiquitination
K209 Ubiquitination
K225 Ubiquitination
K230 Ubiquitination
K248 Ubiquitination
T250 Phosphorylation
K265 Acetylation
K265 Sumoylation
K269 Acetylation
K319 Ubiquitination
K334 Ubiquitination
S388 Phosphorylation
T390 Phosphorylation
K394 Ubiquitination
K396 Ubiquitination
K411 Ubiquitination
K417 Ubiquitination
K420 Ubiquitination
K462 Ubiquitination
K473 Acetylation
K476 Ubiquitination

Research Backgrounds

Function:

Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring-like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2, but that of FGF1.

Subcellular Location:

Nucleus. Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer and homooligomer. Interacts with microtubules. Interacts with FGF2 and RAP80. Does not interact with FGF1 or FGF2 isoform 24 kDa.

Family&Domains:

The C-terminal region mediates the interaction with microtubules and is able to nucleate and bundles microtubules in vitro.

The centrosome localization domain (CLD) region mediates the localization to centrosomes and homooligomerization.

Belongs to the translokin family.

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