Product: CEP55 Antibody
Catalog: DF3918
Description: Rabbit polyclonal antibody to CEP55
Application: WB IHC
Reactivity: Human
Mol.Wt.: 54 KD; 54kD(Calculated).
Uniprot: Q53EZ4
RRID: AB_2836271

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
CEP55 Antibody detects endogenous levels of total CEP55.
RRID:
AB_2836271
Cite Format: Affinity Biosciences Cat# DF3918, RRID:AB_2836271.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C10orf3; cancer/testis antigen 111; Centrosomal protein 55kDa; Centrosomal protein of 55 kDa; CEP 55; Cep55; CEP55_HUMAN; CT111; FLJ10540; Up regulated in colon cancer 6; Up-regulated in colon cancer 6; URCC 6; URCC6;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q53EZ4 CEP55_HUMAN:

Expressed in embryonic brain (PubMed:28264986). Expressed in fetal brain ganglionic eminence, kidney tubules and multinucleate neurons in the temporal cortex (PubMed:28264986). Expressed in adult brain, cerebellum, kidney tubules, intestine and muscles (at protein level) (PubMed:28295209, PubMed:28264986). Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.

Sequence:
MSSRSTKDLIKSKWGSKPSNSKSETTLEKLKGEIAHLKTSVDEITSGKGKLTDKERHRLLEKIRVLEAEKEKNAYQLTEKDKEIQRLRDQLKARYSTTTLLEQLEETTREGERREQVLKALSEEKDVLKQQLSAATSRIAELESKTNTLRLSQTVAPNCFNSSINNIHEMEIQLKDALEKNQQWLVYDQQREVYVKGLLAKIFELEKKTETAAHSLPQQTKKPESEGYLQEEKQKCYNDLLASAKKDLEVERQTITQLSFELSEFRRKYEETQKEVHNLNQLLYSQRRADVQHLEDDRHKTEKIQKLREENDIARGKLEEEKKRSEELLSQVQFLYTSLLKQQEEQTRVALLEQQMQACTLDFENEKLDRQHVQHQLHVILKELRKARNQITQLESLKQLHEFAITEPLVTFQGETENREKVAASPKSPTAALNESLVECPKCNIQYPATEHRDLLVHVEYCSK

PTMs - Q53EZ4 As Substrate

Site PTM Type Enzyme
S5 Phosphorylation
K13 Ubiquitination
K17 Ubiquitination
S21 Phosphorylation
K22 Ubiquitination
S23 Phosphorylation
K29 Ubiquitination
K31 Ubiquitination
K38 Ubiquitination
S40 Phosphorylation
K48 Ubiquitination
T52 Phosphorylation
K92 Ubiquitination
K119 Ubiquitination
K125 Ubiquitination
K129 Ubiquitination
S144 Phosphorylation
K145 Ubiquitination
T146 Phosphorylation
T148 Phosphorylation
S152 Phosphorylation
T154 Phosphorylation
K180 Ubiquitination
K196 Ubiquitination
K201 Ubiquitination
K207 Ubiquitination
K208 Ubiquitination
S215 Phosphorylation
K222 Ubiquitination
Y228 Phosphorylation
K233 Ubiquitination
K235 Ubiquitination
K245 Ubiquitination
K246 Ubiquitination
K268 Ubiquitination
K274 Ubiquitination
Y284 Phosphorylation
S285 Phosphorylation
K300 Ubiquitination
K306 Ubiquitination
K317 Ubiquitination
Y336 Phosphorylation
T337 Phosphorylation
K341 Ubiquitination
T360 Phosphorylation
K367 Ubiquitination
S396 Phosphorylation
K398 Ubiquitination
S425 Phosphorylation P28482 (MAPK1) , P06493 (CDK1)
K427 Ubiquitination
S428 Phosphorylation P06493 (CDK1) , P28482 (MAPK1)
T430 Phosphorylation
S436 Phosphorylation P53350 (PLK1)
K442 Ubiquitination
Y461 Phosphorylation
S463 Phosphorylation

Research Backgrounds

Function:

Plays a role in mitotic exit and cytokinesis. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. Required for successful completion of cytokinesis. Not required for microtubule nucleation. Plays a role in the development of the brain and kidney.

PTMs:

There is a hierachy of phosphorylation, where both Ser-425 and Ser-428 are phosphorylated at the onset of mitosis, prior to Ser-436. Phosphorylation at Ser-425 and Ser-428 is required for dissociation from the centrosome at the G2/M boundary. Phosphorylation at the 3 sites, Ser-425, Ser-428 and Ser-436, is required for protein function at the final stages of cell division to complete cytokinesis successfully.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cleavage furrow. Midbody>Midbody ring.
Note: Present at the centrosomes at interphase. A small portion is associated preferentially with the mother centriole, whereas the majority localizes to the pericentriolar material. During mitosis, loses affinity for the centrosome at the onset of prophase and diffuses throughout the cell. This dissociation from the centrosome is phosphorylation-dependent. May remain localized at the centrosome during mitosis in certain cell types. Appears at the cleavage furrow in late anaphase and in the midbody in cytokinesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in embryonic brain. Expressed in fetal brain ganglionic eminence, kidney tubules and multinucleate neurons in the temporal cortex. Expressed in adult brain, cerebellum, kidney tubules, intestine and muscles (at protein level). Widely expressed, mostly in proliferative tissues. Highly expressed in testis. Intermediate levels in adult and fetal thymus, as well as in various cancer cell lines. Low levels in different parts of the digestive tract, bone marrow, lymph nodes, placenta, fetal heart and fetal spleen. Hardly detected in brain.

Subunit Structure:

Homodimer. Interacts (phosphorylated on Ser-425 and Ser-428) with PLK1. Interacts with AKAP9/CG-NAP; the interaction occurs in interphase and is lost upon mitotic entry. Interacts with PCNT/Kendrin; the interaction occurs in interphase and is lost upon mitotic entry. Directly interacts with PDCD6IP; this interaction is required for PDCD6IP targeting to the midbody; CEP55 binds PDCD6IP in a 2:1 stoichiometry; PDCD6IP competes with TSG101 for the same binding site. Interacts with TSG101; TSG101 competes with PDCD6IP for the same binding site; interaction is required for cytokinesis but not for viral budding. Interacts with MVB12A, VPS37B, VPS37C and VPS28.

References

1). Sharp Downregulation of Hub Genes Associated With the Pathogenesis of Breast Cancer From Ductal Carcinoma In Situ to Invasive Ductal Carcinoma. Frontiers in Oncology, 2021 (PubMed: 34094915) [IF=4.7]

Application: WB    Species: Human    Sample: breast cancer tissue

Figure 6 Protein expression levels of hub genes. (A) Protein expression levels of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 were determined by western blotting. (B) Western blot analysis of CDK1, MELK, CEP55, TOP2A, NUSAP1, PBK, RRM2, and MAD2L1 in tissue from different stages of breast disease, and quantification of the intensity relative to GAPDH. One-way ANOVA was performed to acquire statistical significance (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). NME, normal mammary epithelium; SH, simple ductal hyperplasia; ADH, atypical ductal hyperplasia; DCIS, ductal carcinoma in situ; IDC, invasive ductal carcinoma.

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