CPB2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using CPB2 Antibody.

Product:	CPB2 Antibody
Catalog:	DF3897
Description:	Rabbit polyclonal antibody to CPB2
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	48 KD; 48kD(Calculated).
Uniprot:	Q96IY4
RRID:	AB_2836250

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(91%), Zebrafish(82%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(100%), Dog(91%), Chicken(91%), Xenopus(90%)

Clonality:

Polyclonal

Specificity:

CPB2 Antibody detects endogenous levels of total CPB2.

RRID:

AB_2836250
Cite Format: Affinity Biosciences Cat# DF3897, RRID:AB_2836250.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1110032P04Rik; 4930405E17Rik; bA139H14.2; Carboxypeptidase B like protein; Carboxypeptidase U; CarboxypeptidaseB2; CarboxypeptidaseU; CP B2; CPB 2; CPB2; CPU; PCPB; Plasma carboxypeptidase B; Plasma carboxypeptidase B2; Procarboxypeptidase B; Procarboxypeptidase R; Procarboxypeptidase U; TAFI; Thrombin activable fibrinolysis inhibitor; Thrombin activatable fibrinolysis inhibitor;

Immunogens

Immunogen:

Uniprot:

Q96IY4(CBPB2_HUMAN) >>Visit HPA database.

Gene(ID):

CPB2(1361)

Expression:

Q96IY4 CBPB2_HUMAN:

Plasma; synthesized in the liver.

Sequence:

Q96IY4 CBPB2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKLCSLAVLVPIVLFCEQHVFAFQSGQVLAALPRTSRQVQVLQNLTTTYEIVLWQPVTADLIVKKKQVHFFVNASDVDNVKAHLNVSGIPCSVLLADVEDLIQQQISNDTVSPRASASYYEQYHSLNEIYSWIEFITERHPDMLTKIHIGSSFEKYPLYVLKVSGKEQAAKNAIWIDCGIHAREWISPAFCLWFIGHITQFYGIIGQYTNLLRLVDFYVMPVVNVDGYDYSWKKNRMWRKNRSFYANNHCIGTDLNRNFASKHWCEEGASSSSCSETYCGLYPESEPEVKAVASFLRRNINQIKAYISMHSYSQHIVFPYSYTRSKSKDHEELSLVASEAVRAIEKISKNTRYTHGHGSETLYLAPGGGDDWIYDLGIKYSFTIELRDTGTYGFLLPERYIKPTCREAFAAVSKIAWHVIRNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Dog

Chicken

Horse

Bovine

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96IY4 As Substrate

Q96IY4

Site	PTM Type	Source
N44	N-Glycosylation	Uniprot
N73	N-Glycosylation	Uniprot
N85	N-Glycosylation	Uniprot
N108	N-Glycosylation	Uniprot
N241	N-Glycosylation	Uniprot
S338	Phosphorylation	Uniprot
Y380	Phosphorylation	Uniprot
T383	Phosphorylation	Uniprot

Research Backgrounds

Q96IY4_CBPB2_HUMAN

Function:

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin.

PTMs:

N-glycosylated. N-glycan at Asn-108: Hex5HexNAc4.

Subcellular Location:

Secreted.

Tissue Specificity:

Plasma; synthesized in the liver.

Family&Domains:

Belongs to the peptidase M14 family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

· Organismal Systems > Digestive system > Pancreatic secretion.

· Organismal Systems > Digestive system > Protein digestion and absorption.

Restrictive clause

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For Research Use Only.
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CPB2 Antibody - #DF3897