Product: CBR3 Antibody
Catalog: DF3894
Description: Rabbit polyclonal antibody to CBR3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 31 KD; 31kD(Calculated).
Uniprot: O75828
RRID: AB_2836247

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(90%), Sheep(90%), Rabbit(80%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CBR3 Antibody detects endogenous levels of total CBR3.
RRID:
AB_2836247
Cite Format: Affinity Biosciences Cat# DF3894, RRID:AB_2836247.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Carbonyl reductase (NADPH) 3; Carbonyl reductase (NADPH) 3 EC 1.1.1.184; Carbonyl reductase [NADPH] 3; Carbonyl reductase 3; CBR 3; CBR3; CBR3_HUMAN; EC 1.1.1.184; Epididymis secretory protein Li 25; hCBR3; HEL S 25; NADPH dependent carbonyl reductase 3; NADPH-dependent carbonyl reductase 3; SDR21C2; Short chain dehydrogenase/reductase family 21C member 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O75828 CBR3_HUMAN:

Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.

Sequence:
MSSCSRVALVTGANRGIGLAIARELCRQFSGDVVLTARDVARGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLNVLVNNAAVAFKSDDPMPFDIKAEMTLKTNFFATRNMCNELLPIMKPHGRVVNISSLQCLRAFENCSEDLQERFHSETLTEGDLVDLMKKFVEDTKNEVHEREGWPNSPYGVSKLGVTVLSRILARRLDEKRKADRILVNACCPGPVKTDMDGKDSIRTVEEGAETPVYLALLPPDATEPQGQLVHDKVVQNW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Bovine
90
Sheep
90
Rabbit
80
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75828 As Substrate

Site PTM Type Enzyme
T11 Phosphorylation
S30 Phosphorylation
T36 Phosphorylation
S56 Phosphorylation
Y81 Phosphorylation
S97 Phosphorylation
K112 Ubiquitination
K173 Acetylation
S192 Phosphorylation
Y194 Phosphorylation
K198 Ubiquitination
C226 S-Nitrosylation
C227 S-Nitrosylation
S240 Phosphorylation
Y253 Phosphorylation

Research Backgrounds

Function:

Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro).

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis.

Family&Domains:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Research Fields

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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