CBR1 Antibody | Affinity Biosciences

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FIGURE 4 | The effect of Maternal 25-hydroxyvitamin D deficiency on the expression level of Nrf2 and CBR1 in maternal placenta, liver, and pancreas tissues.

Product:	CBR1 Antibody
Catalog:	DF3893
Description:	Rabbit polyclonal antibody to CBR1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Chicken
Mol.Wt.:	32 KD; 30kD(Calculated).
Uniprot:	P16152
RRID:	AB_2836246

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Chicken(100%)

Clonality:

Polyclonal

Specificity:

CBR1 Antibody detects endogenous levels of total CBR1.

RRID:

AB_2836246
Cite Format: Affinity Biosciences Cat# DF3893, RRID:AB_2836246.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

15 hydroxyprostaglandin dehydrogenase [NADP+]; 15-hydroxyprostaglandin dehydrogenase [NADP+]; Carbonyl reductase [NADPH] 1; Carbonyl Reductase 1; CBR 1; CBR1; CBR1_HUMAN; CRN; NADPH dependent carbonyl reductase 1; NADPH-dependent carbonyl reductase 1; Prostaglandin 9 ketoreductase; Prostaglandin 9-ketoreductase; Prostaglandin E(2) 9 reductase; Prostaglandin-E(2) 9-reductase; SDR21C1;

Immunogens

Immunogen:

Uniprot:

P16152(CBR1_HUMAN) >>Visit HPA database.

Gene(ID):

CBR1(873)

Sequence:

P16152 CBR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Chicken

100

Zebrafish

Bovine

Xenopus

Pig

Horse

Sheep

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16152 As Substrate

P16152

Site	PTM Type	Source
S2	Acetylation	Uniprot
S30	Phosphorylation	Uniprot
T36	Phosphorylation	Uniprot
R42	Methylation	Uniprot
S56	Phosphorylation	Uniprot
K96	Acetylation	Uniprot
T123	Phosphorylation	Uniprot
K130	Ubiquitination	Uniprot
S143	Phosphorylation	Uniprot
K148	Ubiquitination	Uniprot
S149	Phosphorylation	Uniprot
S151	Phosphorylation	Uniprot
K157	Acetylation	Uniprot
K157	Ubiquitination	Uniprot
S160	Phosphorylation	Uniprot
T162	Phosphorylation	Uniprot
K174	Ubiquitination	Uniprot
K181	Acetylation	Uniprot
K186	Acetylation	Uniprot
K186	Ubiquitination	Uniprot
S191	Phosphorylation	Uniprot
Y194	Phosphorylation	Uniprot
K198	Acetylation	Uniprot
S213	Phosphorylation	Uniprot
K220	Ubiquitination	Uniprot
K239	Ubiquitination	Uniprot
K242	Ubiquitination	Uniprot
Y253	Phosphorylation	Uniprot
K272	Ubiquitination	Uniprot

Research Backgrounds

P16152_CBR1_HUMAN

Function:

NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Maternal 25-Hydroxyvitamin D Deficiency Promoted Metabolic Syndrome and Downregulated Nrf2/CBR1 Pathway in Offspring. Frontiers in Pharmacology, 2020 (PubMed: 32184720) [IF=5.6]

Application: WB Species: human Sample: maternal placenta, liver, and pancreas

FIGURE 4 | The effect of Maternal 25-hydroxyvitamin D deficiency on the expression level of Nrf2 and CBR1 in maternal placenta, liver, and pancreas tissues. (A) Nrf2 and CBR1 immunohistochemical staining in maternal placenta, liver, and pancreas tissues obtained in normal group and 25-hydroxyvitamin D deficiency group (magnification,×200). (B–D) Application of Western blot (B, C) and qT-PCR (D) to ascertain the protein and mRNA expression levels, respectively, in maternal placenta, liver, and pancreas Tissues

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CBR1 Antibody - #DF3893