Product: CBR1 Antibody
Catalog: DF3893
Description: Rabbit polyclonal antibody to CBR1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Chicken
Mol.Wt.: 32 KD; 30kD(Calculated).
Uniprot: P16152
RRID: AB_2836246

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Chicken(100%)
Clonality:
Polyclonal
Specificity:
CBR1 Antibody detects endogenous levels of total CBR1.
RRID:
AB_2836246
Cite Format: Affinity Biosciences Cat# DF3893, RRID:AB_2836246.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

15 hydroxyprostaglandin dehydrogenase [NADP+]; 15-hydroxyprostaglandin dehydrogenase [NADP+]; Carbonyl reductase [NADPH] 1; Carbonyl Reductase 1; CBR 1; CBR1; CBR1_HUMAN; CRN; NADPH dependent carbonyl reductase 1; NADPH-dependent carbonyl reductase 1; Prostaglandin 9 ketoreductase; Prostaglandin 9-ketoreductase; Prostaglandin E(2) 9 reductase; Prostaglandin-E(2) 9-reductase; SDR21C1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSSGIHVALVTGGNKGIGLAIVRDLCRLFSGDVVLTARDVTRGQAAVQQLQAEGLSPRFHQLDIDDLQSIRALRDFLRKEYGGLDVLVNNAGIAFKVADPTPFHIQAEVTMKTNFFGTRDVCTELLPLIKPQGRVVNVSSIMSVRALKSCSPELQQKFRSETITEEELVGLMNKFVEDTKKGVHQKEGWPSSAYGVTKIGVTVLSRIHARKLSEQRKGDKILLNACCPGWVRTDMAGPKATKSPEEGAETPVYLALLPPDAEGPHGQFVSEKRVEQW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
100
Zebrafish
75
Bovine
75
Xenopus
63
Pig
0
Horse
0
Sheep
0
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16152 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S30 Phosphorylation
T36 Phosphorylation
R42 Methylation
S56 Phosphorylation
K96 Acetylation
T123 Phosphorylation
K130 Ubiquitination
S143 Phosphorylation
K148 Ubiquitination
S149 Phosphorylation
S151 Phosphorylation
K157 Acetylation
K157 Ubiquitination
S160 Phosphorylation
T162 Phosphorylation
K174 Ubiquitination
K181 Acetylation
K186 Acetylation
K186 Ubiquitination
S191 Phosphorylation
Y194 Phosphorylation
K198 Acetylation
S213 Phosphorylation
K220 Ubiquitination
K239 Ubiquitination
K242 Ubiquitination
Y253 Phosphorylation
K272 Ubiquitination

Research Backgrounds

Function:

NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer.

Family&Domains:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Maternal 25-Hydroxyvitamin D Deficiency Promoted Metabolic Syndrome and Downregulated Nrf2/CBR1 Pathway in Offspring. Frontiers in Pharmacology (PubMed: 32184720) [IF=5.6]

Application: WB    Species: human    Sample: maternal placenta, liver, and pancreas

FIGURE 4 | The effect of Maternal 25-hydroxyvitamin D deficiency on the expression level of Nrf2 and CBR1 in maternal placenta, liver, and pancreas tissues. (A) Nrf2 and CBR1 immunohistochemical staining in maternal placenta, liver, and pancreas tissues obtained in normal group and 25-hydroxyvitamin D deficiency group (magnification,×200). (B–D) Application of Western blot (B, C) and qT-PCR (D) to ascertain the protein and mRNA expression levels, respectively, in maternal placenta, liver, and pancreas Tissues

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