Product: CA3 Antibody
Catalog: DF3889
Description: Rabbit polyclonal antibody to CA3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 38 KD; 30kD(Calculated).
Uniprot: P07451
RRID: AB_2836242

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Horse(92%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CA3 Antibody detects endogenous levels of total CA3.
RRID:
AB_2836242
Cite Format: Affinity Biosciences Cat# DF3889, RRID:AB_2836242.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CA III; CA-III; Ca3; CAH3_HUMAN; CAIII; Car3; Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase III; Carbonic anhydrase III muscle specific; EC 4.2.1.1; Epididymis secretory sperm binding protein Li 167mP; HEL S 167mP;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P07451 CAH3_HUMAN:

Muscle specific.

Sequence:
MAKEWGYASHNGPDHWHELFPNAKGENQSPVELHTKDIRHDPSLQPWSVSYDGGSAKTILNNGKTCRVVFDDTYDRSMLRGGPLPGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNPKYNTFKEALKQRDGIAVIGIFLKIGHENGEFQIFLDALDKIKTKGKEAPFTKFDPSCLFPACRDYWTYQGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAENEPPVPLVSNWRPPQPINNRVVRASFK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
92
Horse
92
Rabbit
92
Bovine
75
Sheep
75
Xenopus
67
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07451 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
S48 Phosphorylation
S50 Phosphorylation
C66 S-Nitrosylation
Y74 Phosphorylation
S99 Phosphorylation
S100 Phosphorylation
Y114 Phosphorylation
K126 Acetylation
Y127 Phosphorylation
T129 Phosphorylation
T216 Phosphorylation
S242 Phosphorylation

Research Backgrounds

Function:

Reversible hydration of carbon dioxide.

PTMs:

S-glutathionylated in hepatocytes under oxidative stress.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Muscle specific.

Family&Domains:

Belongs to the alpha-carbonic anhydrase family.

Research Fields

· Metabolism > Energy metabolism > Nitrogen metabolism.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.