Product: Carbonic Anhydrase 1/CA1 Antibody
Catalog: DF3885
Description: Rabbit polyclonal antibody to Carbonic Anhydrase 1/CA1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 29 KD; 29kD(Calculated).
Uniprot: P00915
RRID: AB_2836238

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Horse(82%), Sheep(91%)
Clonality:
Polyclonal
Specificity:
Carbonic Anhydrase 1/CA1 Antibody detects endogenous levels of total Carbonic Anhydrase 1/CA1.
RRID:
AB_2836238
Cite Format: Affinity Biosciences Cat# DF3885, RRID:AB_2836238.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CA 1; CA I; CA-I; CA1; CAB; CAH1_HUMAN; CAI; CAN; Car 1; Car1; Carbonate dehydratase I; Carbonic anhydrase 1; Carbonic anhydrase A; Carbonic anhydrase B; Carbonic anhydrase B, formerly; Carbonic anhydrase I; Carbonic dehydratase; ECK0125; JW0122; yadF;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTSETKHDTSLKPISVSYNPATAKEIINVGHSFHVNFEDNDNRSVLKGGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNSAKYSSLAEAASKADGLAVIGVLMKVGEANPKLQKVLDALQAIKTKGKRAPFTNFDPSTLLPSSLDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVEGDNAVPMQHNNRPTQPLKGRTVRASF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
91
Sheep
91
Horse
82
Dog
73
Rabbit
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00915 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S30 Phosphorylation
S49 Phosphorylation
S51 Phosphorylation
T56 Phosphorylation
S78 Phosphorylation
S86 Phosphorylation
S88 Phosphorylation
Y89 Phosphorylation
S130 O-Glycosylation
S131 Phosphorylation
S137 Phosphorylation
S218 O-Glycosylation

Research Backgrounds

Function:

Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the alpha-carbonic anhydrase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.