CLIP3 Antibody | Affinity Biosciences

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CLIP3 Antibody - Western blot analysis of extracts from mouse brain, using CLIP3 Antibody.

CLIP3 Antibody - DF3878 staining Hela by IF/ICC.

CLIP3 Antibody - Fig.

Product:	CLIP3 Antibody
Catalog:	DF3878
Description:	Rabbit polyclonal antibody to CLIP3
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	60 KD; 60kD(Calculated).
Uniprot:	Q96DZ5
RRID:	AB_2836231

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(91%), Dog(100%)

Clonality:

Polyclonal

Specificity:

CLIP3 Antibody detects endogenous levels of total CLIP3.

RRID:

AB_2836231
Cite Format: Affinity Biosciences Cat# DF3878, RRID:AB_2836231.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1500005P14Rik; AI844915; CAP GLY domain containing linker protein 3; CAP-Gly domain-containing linker protein 3; CLIP 170 related 59 kDa protein; CLIP-170-related 59 kDa protein; clip3; CLIP3_HUMAN; CLIPR 59; CLIPR-59; CLIPR59; Cytoplasmic linker protein 170 related 59 kDa protein; Cytoplasmic linker protein 170-related 59 kDa protein; Restin like 1; RSNL1;

Immunogens

Immunogen:

Uniprot:

Q96DZ5(CLIP3_HUMAN) >>Visit HPA database.

Gene(ID):

CLIP3(25999)

Sequence:

Q96DZ5 CLIP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTKTDPAPMAPPPRGEEEEEEEEDEPVPEAPSPTQERRQKPVVHPSAPAPLPKDYAFTFFDPNDPACQEILFDPQTTIPELFAIVRQWVPQVQHKIDVIGNEILRRGCHVNDRDGLTDMTLLHYACKAGAHGVGDPAAAVRLSQQLLALGADVTLRSRWTNMNALHYAAYFDVPDLVRVLLKGARPRVVNSTCSDFNHGSALHIAASSLCLGAAKCLLEHGANPALRNRKGQVPAEVVPDPMDMSLDKAEAALVAKELRTLLEEAVPLSCALPKVTLPNYDNVPGNLMLSALGLRLGDRVLLDGQKTGTLRFCGTTEFASGQWVGVELDEPEGKNDGSVGGVRYFICPPKQGLFASVSKISKAVDAPPSSVTSTPRTPRMDFSRVTGKGRREHKGKKKTPSSPSLGSLQQRDGAKAEVGDQVLVAGQKQGIVRFYGKTDFAPGYWYGIELDQPTGKHDGSVFGVRYFTCPPRHGVFAPASRIQRIGGSTDSPGDSVGAKKVHQVTMTQPKRTFTTVRTPKDIASENSISRLLFCCWFPWMLRAEMQS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Horse

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96DZ5 As Substrate

Q96DZ5

Site	PTM Type	Source
K127	Ubiquitination	Uniprot
S245	Phosphorylation	Uniprot
K256	Ubiquitination	Uniprot
K306	Ubiquitination	Uniprot
S373	Phosphorylation	Uniprot
T386	Phosphorylation	Uniprot
T399	Phosphorylation	Uniprot
S401	Phosphorylation	Uniprot
S402	Phosphorylation	Uniprot
S404	Phosphorylation	Uniprot
S407	Phosphorylation	Uniprot
K428	Ubiquitination	Uniprot
K456	Ubiquitination	Uniprot
K500	Ubiquitination	Uniprot
K510	Ubiquitination	Uniprot
T512	Phosphorylation	Uniprot
T514	Phosphorylation	Uniprot
K520	Ubiquitination	Uniprot
S547	Phosphorylation	Uniprot

Research Backgrounds

Q96DZ5_CLIP3_HUMAN

Function:

Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics. May modulate the cellular compartmentalization of AKT kinase family and promote its cell membrane localization, thereby playing a role in glucose transport in adipocytes.

PTMs:

Palmitoylation by ZDHHC17 regulates association with the plasma membrane.

Subcellular Location:

Cell membrane>Lipid-anchor. Cytoplasm. Golgi apparatus>Golgi stack.
Note: Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.

Subunit Structure:

Homodimer. Interacts with AKT1 and AKT2; when AKT1 and AKT2 are phosphorylated and activated, affinity is higher for AKT2. Interacts with ZDHHC13 (via ANK repeats). Interacts with ZDHHC17 (via ANK repeats).

Family&Domains:

Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).

References

1). Effect of CLIP3 Upregulation on Astrocyte Proliferation and Subsequent Glial Scar Formation in the Rat Spinal Cord via STAT3 Pathway After Injury. Journal of Molecular Neuroscience, 2017 (PubMed: 29218499) [IF=2.8]

Application: WB Species: rat Sample: spinal cord

Fig. 1 |Expression of CLIP3 is increased after spinal cord injury.a The BBB scale of rat hind limb locomotor at each time point after injury. b The spinal cord tissues removed from each time point after SCI. Protein immunoblots detected CLIP3 and GAPDH.

Application: IF/ICC Species: rat Sample: astrocytes of the adult spinal cord

Fig. 3 |The upregulation of CLIP3 protein in astrocytes of the adult spinal cord. The immunostaining for CLIP3 with GFAP (a–f), Iba1 (g–l), NeuN(m–n), and O4 (o–p).

Application: IHC Species: rat Sample: spinal cord

Fig. 2| The detection of CLIP3 in the sections of the rat spinal cord with immunohistochemistry staining. Sections were stained with specific antibody for CLIP3 under low-power views in sham-operated (a) and 3-day injury groups (b). Sections were stained with specific antibody for CLIP3 under higher power views in the ventral horn of white matter(c, d) and gry matter (e, f).

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CLIP3 Antibody - #DF3878