CLIP1 Antibody - #DF3876

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Product: CLIP1 Antibody
Catalog: DF3876
Description: Rabbit polyclonal antibody to CLIP1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 161 KD; 162kD(Calculated).
Uniprot: P30622
RRID: AB_2836229

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
CLIP1 Antibody detects endogenous levels of total CLIP1.
RRID:
AB_2836229
Cite Format: Affinity Biosciences Cat# DF3876, RRID:AB_2836229.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CAP GLY domain containing linker protein 1; CAP-Gly domain-containing linker protein 1; CLIP 170; CLIP; CLIP-170; CLIP1; CLIP1_HUMAN; CYLN1; cytoplasmic linker 1; Cytoplasmic linker protein 1; Cytoplasmic linker protein 170 alpha 2; Cytoplasmic linker protein 170 alpha-2; Cytoplasmic linker protein CLIP 170; Reed Steinberg cell expressed intermediate filament associated; Reed Sternberg intermediate filament associated protein 3; Reed-Sternberg intermediate filament-associated protein; Restin; RSN;

Immunogens

Immunogen:
Uniprot:

P30622(CLIP1_HUMAN) >>Visit HPA database.

Gene(ID):
CLIP1(6249)
Expression:
P30622 CLIP1_HUMAN:

Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.

Sequence:
MSMLKPSGLKAPTKILKPGSTALKTPTAVVAPVEKTISSEKASSTPSSETQEEFVDDFRVGERVWVNGNKPGFIQFLGETQFAPGQWAGIVLDEPIGKNDGSVAGVRYFQCEPLKGIFTRPSKLTRKVQAEDEANGLQTTPASRATSPLCTSTASMVSSSPSTPSNIPQKPSQPAAKEPSATPPISNLTKTASESISNLSEAGSIKKGERELKIGDRVLVGGTKAGVVRFLGETDFAKGEWCGVELDEPLGKNDGAVAGTRYFQCQPKYGLFAPVHKVTKIGFPSTTPAKAKANAVRRVMATTSASLKRSPSASSLSSMSSVASSVSSRPSRTGLLTETSSRYARKISGTTALQEALKEKQQHIEQLLAERDLERAEVAKATSHVGEIEQELALARDGHDQHVLELEAKMDQLRTMVEAADREKVELLNQLEEEKRKVEDLQFRVEEESITKGDLEQKSQISEDPENTQTKLEHARIKELEQSLLFEKTKADKLQRELEDTRVATVSEKSRIMELEKDLALRVQEVAELRRRLESNKPAGDVDMSLSLLQEISSLQEKLEVTRTDHQREITSLKEHFGAREETHQKEIKALYTATEKLSKENESLKSKLEHANKENSDVIALWKSKLETAIASHQQAMEELKVSFSKGLGTETAEFAELKTQIEKMRLDYQHEIENLQNQQDSERAAHAKEMEALRAKLMKVIKEKENSLEAIRSKLDKAEDQHLVEMEDTLNKLQEAEIKVKELEVLQAKCNEQTKVIDNFTSQLKATEEKLLDLDALRKASSEGKSEMKKLRQQLEAAEKQIKHLEIEKNAESSKASSITRELQGRELKLTNLQENLSEVSQVKETLEKELQILKEKFAEASEEAVSVQRSMQETVNKLHQKEEQFNMLSSDLEKLRENLADMEAKFREKDEREEQLIKAKEKLENDIAEIMKMSGDNSSQLTKMNDELRLKERDVEELQLKLTKANENASFLQKSIEDMTVKAEQSQQEAAKKHEEEKKELERKLSDLEKKMETSHNQCQELKARYERATSETKTKHEEILQNLQKTLLDTEDKLKGAREENSGLLQELEELRKQADKAKAAQTAEDAMQIMEQMTKEKTETLASLEDTKQTNAKLQNELDTLKENNLKNVEELNKSKELLTVENQKMEEFRKEIETLKQAAAQKSQQLSALQEENVKLAEELGRSRDEVTSHQKLEEERSVLNNQLLEMKKRESKFIKDADEEKASLQKSISITSALLTEKDAELEKLRNEVTVLRGENASAKSLHSVVQTLESDKVKLELKVKNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKNQDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDLHDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDETF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Chicken
82
Xenopus
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30622 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
T25 Phosphorylation
S43 Phosphorylation
S44 Phosphorylation
T45 Phosphorylation
S47 Phosphorylation
S48 Phosphorylation
T50 Phosphorylation
T139 Phosphorylation
T140 Phosphorylation
S143 Phosphorylation
T146 Phosphorylation
S147 Phosphorylation
S158 Phosphorylation
S160 Phosphorylation
S162 Phosphorylation
T163 Phosphorylation
S172 Phosphorylation
S180 Phosphorylation
T182 Phosphorylation
S186 Phosphorylation
T189 Phosphorylation
T191 Phosphorylation
S193 Phosphorylation
S195 Phosphorylation P53350 (PLK1)
S197 Phosphorylation
S200 Phosphorylation
S204 Phosphorylation
T260 Phosphorylation
Y262 Phosphorylation
Y269 Phosphorylation
T279 Phosphorylation
S285 Phosphorylation
T286 Phosphorylation
T287 Phosphorylation
S306 Phosphorylation
S310 Phosphorylation
S312 Phosphorylation Q6P5Z2 (PKN3) , P17252 (PRKCA) , Q16512 (PKN1) , P53350 (PLK1)
S314 Phosphorylation
S315 Phosphorylation
S317 Phosphorylation
S320 Phosphorylation
S325 Phosphorylation
S328 Phosphorylation
T333 Phosphorylation
S348 Phosphorylation
T350 Phosphorylation
T351 Phosphorylation
K360 Ubiquitination
K409 Ubiquitination
T501 Phosphorylation
S510 Phosphorylation
K574 Ubiquitination
K589 Ubiquitination
S607 Phosphorylation
K647 Ubiquitination
K751 Ubiquitination
K767 Ubiquitination
S783 Phosphorylation
S784 Phosphorylation
S788 Phosphorylation
S815 Phosphorylation
T833 Phosphorylation
S840 Phosphorylation
S843 Phosphorylation
T848 Phosphorylation
K967 Ubiquitination
S973 Phosphorylation
K1007 Acetylation
S1009 Phosphorylation
K1013 Acetylation
S1066 Phosphorylation
T1087 Phosphorylation
S1140 Phosphorylation
S1169 Phosphorylation
S1230 Phosphorylation
S1234 Phosphorylation
S1236 Phosphorylation
S1239 Phosphorylation
K1245 Ubiquitination
S1302 Phosphorylation
S1303 Phosphorylation
S1304 Phosphorylation
S1305 Phosphorylation
T1308 Phosphorylation
T1310 Phosphorylation
S1364 Phosphorylation P53350 (PLK1) , P68400 (CSNK2A1)

Research Backgrounds

Function:

Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking.

PTMs:

Phosphorylated. Phosphorylation induces conformational changes by increasing the affinity of the N-terminus for C-terminus, resulting in inhibition of its function thus decreasing its binding to microtubules and DCTN1. Exhibits a folded, autoinhibited conformation when phosphorylated and an open conformation when dephosphorylated with increased binding affinity to microtubules and DCTN1. Phosphorylation regulates its recruitment to tyrosinated microtubules and the recruitment of vesicular cargo to microtubules in neurons (By similarity). Phosphorylation by MTOR may positively regulate CLIP1 association with microtubules.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Ruffle.
Note: Localizes to microtubule plus ends (PubMed:21646404, PubMed:17889670). Localizes preferentially to the ends of tyrosinated microtubules (By similarity). Accumulates in plasma membrane regions with ruffling and protrusions. Associates with the membranes of intermediate macropinocytic vesicles (PubMed:12433698).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.

Subunit Structure:

Interacts with MTOR; phosphorylates and regulates CLIP1. Interacts (via CAP-Gly domains) with tubulin. Interacts with SLAIN2. Interacts (via zinc finger) with DCTN1. Interacts with TUBA1B, MAPRE1 and MAPRE3. Binds preferentially to tyrosinated microtubules, and only marginally to detyrosinated microtubules (By similarity).

Family&Domains:

Intramolecular interaction between the zinc finger domain and the CAP-Gly domains may inhibit interaction with tubulin.

Research Fields

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

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