Product: ABCC3 Antibody
Catalog: DF3874
Description: Rabbit polyclonal antibody to ABCC3
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Horse
Mol.Wt.: 170 KD; 169kD(Calculated).
Uniprot: O15438
RRID: AB_2836227

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Horse(82%)
Clonality:
Polyclonal
Specificity:
ABCC3 Antibody detects endogenous levels of total ABCC3.
RRID:
AB_2836227
Cite Format: Affinity Biosciences Cat# DF3874, RRID:AB_2836227.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABC 31; ABC31; ABCC 3; ABCC3; ATP binding cassette sub family C (CFTR/MRP) member 3; ATP binding cassette sub family C member 3; ATP-binding cassette sub-family C member 3; Canalicular multispecific organic anion transporter 2; Canicular multispecific organic anion transporter; CMOAT 2; CMOAT2; EST90757; MLP 2; MLP2; MOAT D; MOAT-D; MOATD; MRP 3; MRP3_HUMAN; Multi specific organic anion transporter D; Multi-specific organic anion transporter D; Multidrug resistance associated protein 3; Multidrug resistance associated protein; Multidrug resistance-associated protein 3; Multispecific organic anion tranporter D;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15438 MRP3_HUMAN:

Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.

Sequence:
MDALCGSGELGSKFWDSNLSVHTENPDLTPCFQNSLLAWVPCIYLWVALPCYLLYLRHHCRGYIILSHLSKLKMVLGVLLWCVSWADLFYSFHGLVHGRAPAPVFFVTPLVVGVTMLLATLLIQYERLQGVQSSGVLIIFWFLCVVCAIVPFRSKILLAKAEGEISDPFRFTTFYIHFALVLSALILACFREKPPFFSAKNVDPNPYPETSAGFLSRLFFWWFTKMAIYGYRHPLEEKDLWSLKEEDRSQMVVQQLLEAWRKQEKQTARHKASAAPGKNASGEDEVLLGARPRPRKPSFLKALLATFGSSFLISACFKLIQDLLSFINPQLLSILIRFISNPMAPSWWGFLVAGLMFLCSMMQSLILQHYYHYIFVTGVKFRTGIMGVIYRKALVITNSVKRASTVGEIVNLMSVDAQRFMDLAPFLNLLWSAPLQIILAIYFLWQNLGPSVLAGVAFMVLLIPLNGAVAVKMRAFQVKQMKLKDSRIKLMSEILNGIKVLKLYAWEPSFLKQVEGIRQGELQLLRTAAYLHTTTTFTWMCSPFLVTLITLWVYVYVDPNNVLDAEKAFVSVSLFNILRLPLNMLPQLISNLTQASVSLKRIQQFLSQEELDPQSVERKTISPGYAITIHSGTFTWAQDLPPTLHSLDIQVPKGALVAVVGPVGCGKSSLVSALLGEMEKLEGKVHMKGSVAYVPQQAWIQNCTLQENVLFGKALNPKRYQQTLEACALLADLEMLPGGDQTEIGEKGINLSGGQRQRVSLARAVYSDADIFLLDDPLSAVDSHVAKHIFDHVIGPEGVLAGKTRVLVTHGISFLPQTDFIIVLADGQVSEMGPYPALLQRNGSFANFLCNYAPDEDQGHLEDSWTALEGAEDKEALLIEDTLSNHTDLTDNDPVTYVVQKQFMRQLSALSSDGEGQGRPVPRRHLGPSEKVQVTEAKADGALTQEEKAAIGTVELSVFWDYAKAVGLCTTLAICLLYVGQSAAAIGANVWLSAWTNDAMADSRQNNTSLRLGVYAALGILQGFLVMLAAMAMAAGGIQAARVLHQALLHNKIRSPQSFFDTTPSGRILNCFSKDIYVVDEVLAPVILMLLNSFFNAISTLVVIMASTPLFTVVILPLAVLYTLVQRFYAATSRQLKRLESVSRSPIYSHFSETVTGASVIRAYNRSRDFEIISDTKVDANQRSCYPYIISNRWLSIGVEFVGNCVVLFAALFAVIGRSSLNPGLVGLSVSYSLQVTFALNWMIRMMSDLESNIVAVERVKEYSKTETEAPWVVEGSRPPEGWPPRGEVEFRNYSVRYRPGLDLVLRDLSLHVHGGEKVGIVGRTGAGKSSMTLCLFRILEAAKGEIRIDGLNVADIGLHDLRSQLTIIPQDPILFSGTLRMNLDPFGSYSEEDIWWALELSHLHTFVSSQPAGLDFQCSEGGENLSVGQRQLVCLARALLRKSRILVLDEATAAIDLETDNLIQATIRTQFDTCTVLTIAHRLNTIMDYTRVLVLDKGVVAEFDSPANLIAARGIFYGMARDAGLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
82
Bovine
55
Sheep
55
Pig
44
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15438 As Substrate

Site PTM Type Enzyme
K160 Ubiquitination
Y229 Phosphorylation
Y231 Phosphorylation
K238 Ubiquitination
K244 Ubiquitination
K265 Methylation
K271 Ubiquitination
K278 Ubiquitination
S281 Phosphorylation
K392 Ubiquitination
K401 Ubiquitination
K479 Ubiquitination
K489 Ubiquitination
K499 Ubiquitination
K512 Ubiquitination
S668 Phosphorylation
K684 Ubiquitination
Y720 Phosphorylation
S767 Phosphorylation
K803 Ubiquitination
T882 Phosphorylation
S884 Phosphorylation
T887 Phosphorylation
T896 Phosphorylation
S908 Phosphorylation
S911 Phosphorylation
S912 Phosphorylation
K931 Ubiquitination
K938 Ubiquitination
K1052 Ubiquitination
S1141 Phosphorylation
K1177 Ubiquitination
S1248 Phosphorylation
K1344 Ubiquitination
K1498 Ubiquitination

Research Backgrounds

Function:

May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity).

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

References

1). Curcumol enhances the sensitivity of doxorubicin in triple-negative breast cancer via regulating the miR-181b-2-3p-ABCC3 axis. BIOCHEMICAL PHARMACOLOGY, 2020 (PubMed: 31926937) [IF=5.8]

Application: WB    Species: human    Sample: MDA-MB-231 and MDA-MB-231/ADR cells

Fig. 3.| ABCC3 is a direct target of miR-181b-2-3p.(F) Western blotting analysis of ABCC3 expression in MDA-MB-231 and MDA-MB-231/ADR cells; GAPDH was used as a loading control.

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