ABCC3 Antibody | Affinity Biosciences

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Product:	ABCC3 Antibody
Catalog:	DF3874
Description:	Rabbit polyclonal antibody to ABCC3
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Horse
Mol.Wt.:	170 KD; 169kD(Calculated).
Uniprot:	O15438
RRID:	AB_2836227

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Horse(82%)

Clonality:

Polyclonal

Specificity:

ABCC3 Antibody detects endogenous levels of total ABCC3.

RRID:

AB_2836227
Cite Format: Affinity Biosciences Cat# DF3874, RRID:AB_2836227.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ABC 31; ABC31; ABCC 3; ABCC3; ATP binding cassette sub family C (CFTR/MRP) member 3; ATP binding cassette sub family C member 3; ATP-binding cassette sub-family C member 3; Canalicular multispecific organic anion transporter 2; Canicular multispecific organic anion transporter; CMOAT 2; CMOAT2; EST90757; MLP 2; MLP2; MOAT D; MOAT-D; MOATD; MRP 3; MRP3_HUMAN; Multi specific organic anion transporter D; Multi-specific organic anion transporter D; Multidrug resistance associated protein 3; Multidrug resistance associated protein; Multidrug resistance-associated protein 3; Multispecific organic anion tranporter D;

Immunogens

Immunogen:

Uniprot:

O15438(MRP3_HUMAN) >>Visit HPA database.

Gene(ID):

ABCC3(8714)

Expression:

O15438 MRP3_HUMAN:

Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.

Sequence:

O15438 MRP3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDALCGSGELGSKFWDSNLSVHTENPDLTPCFQNSLLAWVPCIYLWVALPCYLLYLRHHCRGYIILSHLSKLKMVLGVLLWCVSWADLFYSFHGLVHGRAPAPVFFVTPLVVGVTMLLATLLIQYERLQGVQSSGVLIIFWFLCVVCAIVPFRSKILLAKAEGEISDPFRFTTFYIHFALVLSALILACFREKPPFFSAKNVDPNPYPETSAGFLSRLFFWWFTKMAIYGYRHPLEEKDLWSLKEEDRSQMVVQQLLEAWRKQEKQTARHKASAAPGKNASGEDEVLLGARPRPRKPSFLKALLATFGSSFLISACFKLIQDLLSFINPQLLSILIRFISNPMAPSWWGFLVAGLMFLCSMMQSLILQHYYHYIFVTGVKFRTGIMGVIYRKALVITNSVKRASTVGEIVNLMSVDAQRFMDLAPFLNLLWSAPLQIILAIYFLWQNLGPSVLAGVAFMVLLIPLNGAVAVKMRAFQVKQMKLKDSRIKLMSEILNGIKVLKLYAWEPSFLKQVEGIRQGELQLLRTAAYLHTTTTFTWMCSPFLVTLITLWVYVYVDPNNVLDAEKAFVSVSLFNILRLPLNMLPQLISNLTQASVSLKRIQQFLSQEELDPQSVERKTISPGYAITIHSGTFTWAQDLPPTLHSLDIQVPKGALVAVVGPVGCGKSSLVSALLGEMEKLEGKVHMKGSVAYVPQQAWIQNCTLQENVLFGKALNPKRYQQTLEACALLADLEMLPGGDQTEIGEKGINLSGGQRQRVSLARAVYSDADIFLLDDPLSAVDSHVAKHIFDHVIGPEGVLAGKTRVLVTHGISFLPQTDFIIVLADGQVSEMGPYPALLQRNGSFANFLCNYAPDEDQGHLEDSWTALEGAEDKEALLIEDTLSNHTDLTDNDPVTYVVQKQFMRQLSALSSDGEGQGRPVPRRHLGPSEKVQVTEAKADGALTQEEKAAIGTVELSVFWDYAKAVGLCTTLAICLLYVGQSAAAIGANVWLSAWTNDAMADSRQNNTSLRLGVYAALGILQGFLVMLAAMAMAAGGIQAARVLHQALLHNKIRSPQSFFDTTPSGRILNCFSKDIYVVDEVLAPVILMLLNSFFNAISTLVVIMASTPLFTVVILPLAVLYTLVQRFYAATSRQLKRLESVSRSPIYSHFSETVTGASVIRAYNRSRDFEIISDTKVDANQRSCYPYIISNRWLSIGVEFVGNCVVLFAALFAVIGRSSLNPGLVGLSVSYSLQVTFALNWMIRMMSDLESNIVAVERVKEYSKTETEAPWVVEGSRPPEGWPPRGEVEFRNYSVRYRPGLDLVLRDLSLHVHGGEKVGIVGRTGAGKSSMTLCLFRILEAAKGEIRIDGLNVADIGLHDLRSQLTIIPQDPILFSGTLRMNLDPFGSYSEEDIWWALELSHLHTFVSSQPAGLDFQCSEGGENLSVGQRQLVCLARALLRKSRILVLDEATAAIDLETDNLIQATIRTQFDTCTVLTIAHRLNTIMDYTRVLVLDKGVVAEFDSPANLIAARGIFYGMARDAGLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Pig

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15438 As Substrate

O15438

Site	PTM Type	Source
K160	Ubiquitination	Uniprot
Y229	Phosphorylation	Uniprot
Y231	Phosphorylation	Uniprot
K238	Ubiquitination	Uniprot
K244	Ubiquitination	Uniprot
K265	Methylation	Uniprot
K271	Ubiquitination	Uniprot
K278	Ubiquitination	Uniprot
S281	Phosphorylation	Uniprot
K392	Ubiquitination	Uniprot
K401	Ubiquitination	Uniprot
K479	Ubiquitination	Uniprot
K489	Ubiquitination	Uniprot
K499	Ubiquitination	Uniprot
K512	Ubiquitination	Uniprot
S668	Phosphorylation	Uniprot
K684	Ubiquitination	Uniprot
Y720	Phosphorylation	Uniprot
S767	Phosphorylation	Uniprot
K803	Ubiquitination	Uniprot
T882	Phosphorylation	Uniprot
S884	Phosphorylation	Uniprot
T887	Phosphorylation	Uniprot
T896	Phosphorylation	Uniprot
S908	Phosphorylation	Uniprot
S911	Phosphorylation	Uniprot
S912	Phosphorylation	Uniprot
K931	Ubiquitination	Uniprot
K938	Ubiquitination	Uniprot
K1052	Ubiquitination	Uniprot
S1141	Phosphorylation	Uniprot
K1177	Ubiquitination	Uniprot
S1248	Phosphorylation	Uniprot
K1344	Ubiquitination	Uniprot
K1498	Ubiquitination	Uniprot

Research Backgrounds

O15438_MRP3_HUMAN

Function:

May act as an inducible transporter in the biliary and intestinal excretion of organic anions. Acts as an alternative route for the export of bile acids and glucuronides from cholestatic hepatocytes (By similarity).

Subcellular Location:

Membrane>Multi-pass membrane protein.

Tissue Specificity:

Mainly expressed in the liver. Also expressed in small intestine, colon, prostate, testis, brain and at a lower level in the kidney.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

References

1). Curcumol enhances the sensitivity of doxorubicin in triple-negative breast cancer via regulating the miR-181b-2-3p-ABCC3 axis. BIOCHEMICAL PHARMACOLOGY, 2020 (PubMed: 31926937) [IF=5.8]

Application: WB Species: human Sample: MDA-MB-231 and MDA-MB-231/ADR cells

Fig. 3.| ABCC3 is a direct target of miR-181b-2-3p.(F) Western blotting analysis of ABCC3 expression in MDA-MB-231 and MDA-MB-231/ADR cells; GAPDH was used as a loading control.

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For Research Use Only.
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ABCC3 Antibody - #DF3874